TITLE

A PCR-RFLP method for the simultaneous detection of Babesia bigemina and Theileria annulata infections in cattle

AUTHOR(S)
Ravindran, R.; Saravanan, B. C.; Rao, J. R.; Mishra, A. K.; Bansal, G. C.; Ray, D.
PUB. DATE
December 2007
SOURCE
Current Science (00113891);12/25/2007, Vol. 93 Issue 12, p1840
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Mixed infections due to Theileria annulata and Babesia bigemina are responsible for widespread morbidity and mortality in cattle and buffalo of the tropics and subtropics. In the present communication, a method for the differentiation of these two common tick-borne infections is described. A polymerase chain reaction (PCR) for the amplification of partial 18S small subunit ribosomal DNA fragment from the genomes of B. bigemina and T. annulata was standardized. The PCR amplified a monomorphic DNA fragment of 564 bp size, which was sequenced and analysed for the restriction enzyme differentiating these two haemoprotozoans in the presence of the host DNA. The enzyme Cfr131 was found to differentiate the two species.
ACCESSION #
28544456

 

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