TITLE

Growth cone response to ephrin gradients produced by microfluidic networks

AUTHOR(S)
Lang, Susanne; Von Philipsborn, Anne C.; Bernard, André; Bonhoeffer, Friedrich; Bastmeyer, Martin
PUB. DATE
February 2008
SOURCE
Analytical & Bioanalytical Chemistry;Feb2008, Vol. 390 Issue 3, p809
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A microfluidic network (μFN) etched into a silicon wafer was used to deliver protein solutions containing different concentrations of the axonal guidance molecule ephrinA5 onto a silicone stamp. In a subsequent microcontact printing (μCP) step, the protein was transferred onto a polystyrene culture dish. In this way, stepwise substrate-bound concentration gradients of ephrinA5 were fabricated spanning a total distance of 320 μm. We tested the response of chick retinal ganglion cell (RGC) axons, which are guided in vivo by ephrin gradients, to these in vitro gradients. Temporal, but not nasal axons stop at a distinct zone in the gradient, which is covered with a certain surface density of substrate-bound ephrinA5. Within the temporal RGC population, all axons respond uniformly to the gradients tested. The position of the stop zone depends on the slope of the gradient with axons growing further into the gradient in shallow gradients than in steep gradients. However, axons stop at lower ephrinA5 concentrations in shallow gradients than in steep gradients, indicating that the growth cone can adjust its sensitivity during the detection of a concentration gradient of ephrinA5.
ACCESSION #
28404800

 

Related Articles

  • Microfluidic four-roll mill for all flow types. Lee, Joo Sung; Dylla-Spears, Rebecca; Teclemariam, Nerayo P.; Muller, Susan J. // Applied Physics Letters;2/12/2007, Vol. 90 Issue 7, p074103 

    A microfluidic four-roll mill device that can cover the entire spectrum of flow types including purely rotational flow was designed using pseudo-three-dimensional simulations. In experiments using high aspect ratio devices etched in silicon, the authors observed the whole range of flow type by...

  • Rat chronic glaucoma model induced by intracameral injection of microbeads suspended in sodium sulfate-sodium hyaluronate. Matsumoto, Yoshiko; Kanamori, Akiyasu; Nakamura, Makoto; Negi, Akira // Japanese Journal of Ophthalmology;May2014, Vol. 58 Issue 3, p290 

    Purpose: To establish a rat glaucoma model with chronic intraocular pressure (IOP) elevation induced by microbeads suspended in sodium sulfate-sodium hyaluronate. Methods: Chronic elevation of IOP was induced unilaterally by injecting polystyrene microbeads, suspended in 4 % sodium sulfate and 3...

  • Controlled nonuniformity in macroporous silicon pore growth. Matthias, Sven; Müller, Frank; Gösele, Ulrich // Applied Physics Letters;11/28/2005, Vol. 87 Issue 22, p224106 

    Photoelectrochemical etching of uniform prestructured silicon wafers in hydrofluoric acid containing solutions yields periodic structures that can be applied to two- and three-dimensional photonic crystals or microfluidics. Here we demonstrate experimentally macroporous silicon etching initiated...

  • Understanding Nerve Fiber Layer Analysis.  // Review of Optometry;10/15/2002 Supplement, Vol. 139, p38A 

    Focuses on the use of ophthalmoscope in determining the reason of the loss of retinal ganglion cells. Measurement of the retinal nerve fiber layer; Risks of blindness; Way of treating the patients.

  • Molecular mechanisms of RGC death sought. Merbs, Shannath L. // Ophthalmology Times;08/15/97, Vol. 22 Issue 16, p38 

    Emphasizes the need for a better understanding of molecular mechanisms that lead to apoptosis of the retinal ganglion cells (RCG). Theories on the factors triggering apoptosis following optic nerve injury; Role of reactive oxygen intermediates in the proposed cascade leading to RGC death.

  • Neuroprotection may prevent retinal ganglion cell death. Charters, Lynda // Ophthalmology Times;5/15/2002, Vol. 27 Issue 10, p78 

    Discusses the role of neuroprotection in optic nerve disease in the prevention of retinal ganglion cell death. Causes of optic neuropathy; Evidence that exists for neuroprotection.

  • New glaucoma therapy turns toward neuroprotective. Charters, Lynda // Ophthalmology Times;07/15/99, Vol. 24 Issue 14, p20 

    Focuses on pharmacological therapies studied to evaluate their ability to prevent the death of retinal ganglion cells in glaucomatous optic neuropathy. How to prevent the start of the apoptotic process or programmed cell death; Factors which may trigger the commencement of cell death; Drugs...

  • Correction: Retinal Ganglion Cells Can Rapidly Change Polarity from Off to On. Geffen, Maria Neimark; de Vries, Saskia E. J.; Meister, Markus // PLoS Biology;May2007, Vol. 5 Issue 5, pe136 

    NA

  • Absence of spectrally specific lateral inputs to midget ganglion cells in primate retina. Calkins, David J.; Sterling, Peter // Nature;6/13/1996, Vol. 381 Issue 6583, p613 

    Reports on the role of the retinal ganglion cells in the conveyance of visual information to the brain. Identification of the amacrine cell processes presynaptic to the ganglion cells and midget bipolar cells; Determination whether there could be cone-selective inputs to other regions of the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics