TITLE

Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques

AUTHOR(S)
Xunli Zhang; Huabing Yin; Cooper, Jon M.; Haswell, Stephen J.
PUB. DATE
February 2008
SOURCE
Analytical & Bioanalytical Chemistry;Feb2008, Vol. 390 Issue 3, p833
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes. [Figure not available: see fulltext.]
ACCESSION #
28404798

 

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