TITLE

The structural modification of DNA nucleosides by nonenzymatic glycation: an in vitro study based on the reactions of glyoxal and methylglyoxal with 2′-deoxyguanosine

AUTHOR(S)
Li, Yuyuan; Cohenford, Menashi A.; Dutta, Udayan; Dain, Joel A.
PUB. DATE
January 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 2, p679
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Methylglyoxal and glyoxal are generated from the oxidation of carbohydrates and lipids, and like d-glucose have been shown to nonenzymatically react with proteins to form advanced glycation end products (AGEs). AGEs can occur both in vitro and in vivo, and these compounds have been shown to exacerbate many of the long-term complications of diabetes. Earlier studies in our laboratory reported d-glucose, d-galactose, and d/l-glyceraldehyde formed AGEs with nucleosides. The objective of this study was to focus on purines and pyrimidines and to analyze these DNA nucleoside derived AGE adducts with glyoxal or methylglyoxal using a combination of analytical techniques. Studies using UV and fluorescence spectroscopy along with mass spectrometry provided for a thorough analysis of the nucleoside AGEs and demonstrated that methylglyoxal and glyoxal reacted with 2′-deoxyguanosine via the classic Amadori pathway, and did not react appreciably with 2′-deoxyadenosine, 2′-deoxythymidine, and 2′-deoxycytidine. Additional findings revealed that methylglyoxal was more reactive than glyoxal.
ACCESSION #
28110529

 

Related Articles

  • A New Role of Glucose-6-Phosphate Isomerase: Protection of Cell Structures from Malonic Dialdehyde. Agadzhanyan, Z.; Dmitriev, L. // Doklady Biochemistry & Biophysics;Jul2005, Vol. 403 Issue 1-6, p281 

    Assesses the possibility of malonic dialdehyde (MDA) conversion into methylglyoxal and then into D-lactate. Sequence of reactions in which methylglyoxal functions as an intermediate compound; Ways in which the level of MDA in the course of iron-ascorbate-induced lipid peroxidation was...

  • Molybdate sulfuric acid-catalyzed one-pot synthesis of substituted coumarins under solvent-free conditions. Khodabakhshi, Saeed; Karami, Bahador; Eskandari, Khalil // Research on Chemical Intermediates;Oct2015, Vol. 41 Issue 10, p7263 

    An efficient method for the synthesis of new aryloylamido coumarins via a one-pot, three-component reaction of an aryl glyoxal, benzamide, and a 4-hydroxycoumarin in the presence of molybdate sulfuric acid under solvent-free conditions is reported. This high yielding reaction is in accordance...

  • Analytical study proving alprazolam degradation to its main impurity triazolaminoquinoleine through Maillard reaction. Huidobro, A. L.; Barbas, C. // Analytical & Bioanalytical Chemistry;Jul2009, Vol. 394 Issue 5, p1349 

    Triazolaminoquinoleine is rapidly formed in formulations of alprazolam tablets in presence of excipients, and its generation is speeded up with increasing temperature and humidity. The present paper deals with detailed quantitative and qualitative studies into the nonactive constituents of the...

  • Crosslinking reaction of poly(vinyl alcohol) with glyoxal. Yun Zhang; Zhu, Peter C.; Edgren, David // Journal of Polymer Research;Sep2010, Vol. 17 Issue 5, p725 

    Crosslinking reactions are one of the most commonly used techniques to improve physical properties of polyvinyl alcohol (PVA). Commonly used crosslinkers for these reactions are di-functional compounds, such as glutaraldehyde and glyoxal. The hydroxyl groups of PVA react with aldehydes via...

  • Biochemical Indices of Severity in Human Malaria. Asagba, S. O.; Eriyamremu, G. E.; George, B. O.; Okoro, I. // Journal of Medical Sciences;2010, Vol. 10 Issue 4, p87 

    The present study highlights the potential of using lactate dehydrogenase (LDH, EC. 1.1.1.27) and aldehyde oxidase (AO, E.C.1.2.3.1.) activities and level of lipid peroxidation (LPO) in RBC as indices in monitoring the severity of P. falciparum malaria infections. Two hundred and forty one male...

  • α-Oxoaldehydes in haemodialysis solution. Nakayama, Keisuke; Nakayama, Masaaki; Terawaki, Hiroyuki; Satoh, Toshinobu; Kohno, Masahiro; Ito, Sadayoshi // Nephrology Dialysis Transplantation;Jul2007, Vol. 22 Issue 7, p2097 

    The article presents information on a study which looks at the presence of glucose-derived α-oxoaldehydes, such as methylglyoxal (MG) and glyoxal (GO), in haemodialysis (HD) solutions. In this study, the authors measured MG and GO levels in the commercially available brands of liquid and...

  • Organisation of microbicide data: 2 Aldehydes.  // Directory of Microbicides for the Protection of Materials: A Han;2005, p459 

    The article deals with aldehyde microbicidals including formaldehyde, acetaldehyde, glyoxal, succinaldehyde and 2-propenal among others. It offers information on their chemical and structural formulas, molecular mass, chemical and physical properties, toxicity and antimicrobial effectiveness and...

  • Capacity of Glyoxal Is in Serious Surplus.  // China Chemical Reporter;4/16/2009, Vol. 20 Issue 11, p25 

    The article focuses on the capacity and consumption of glyoxal in China. The actual output of glyoxal continues to increase with combined capacity of around 110,000 t/a. and is expected to increase further to 140,000 t/a in 2012. The consumption of glyoxal has increased constantly because of its...

  • The continental source of glyoxal estimated by the synergistic use of spaceborne measurements and inverse modelling. Stavrakou, T.; Müller, J.-F.; De Smedt, I.; Van Roozendael, M.; Kanakidou, M.; Vrekoussis, M.; Wittrock, F.; Richter, A.; Burrows, J. P. // Atmospheric Chemistry & Physics Discussions;2009, Vol. 9 Issue 3, p13593 

    Tropospheric glyoxal and formaldehyde columns retrieved from the SCIAMACHY satellite instrument in 2005 are used with the IMAGESv2 global chemistry-transport model and its adjoint in a two-compound inversion scheme designed to estimate the continental source of glyoxal. The formaldehyde...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics