TITLE

Novel gel-based rapid test for non-instrumental detection of ochratoxin A in beer

AUTHOR(S)
Goryacheva, I. Y.; Basova, E. Y.; Van Peteghem, C.; Eremin, S. A.; Pussemier, L.; Motte, J.-C.; De Saeger, S.
PUB. DATE
January 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 2, p723
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A rapid easy-to-use immunoassay was optimised for the non-instrumental detection of ochratoxin A (OTA) in beer. The analytical method involves preconcentration on the immunoaffinity layer inside a column followed by direct competitive ELISA detection in the same layer. The visual cut-off value, i.e. the lowest OTA concentration resulting in no colour development, was 0.2 μg L-1. Assay validation was performed using samples spiked with OTA. Thirty-seven naturally contaminated samples were screened with the gel-based method developed and no false-negative results were obtained. The method described offers a simple, rapid and cost-effective screening tool, thus contributing to better health protection of consumers. [Figure not available: see fulltext.]
ACCESSION #
28110525

 

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