TITLE

Serum IgA1 from IgA nephropathy patients induces apoptosis in podocytes through direct and indirect pathways

AUTHOR(S)
Cheng Wang; Hui Peng; Hua Tang; Xun Liu; Zhujiang Chen; Tanqi Lou; Xueqing Yu
PUB. DATE
December 2007
SOURCE
Clinical & Investigative Medicine;Dec2007, Vol. 30 Issue 6, pE240
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To investigate apoptosis of podocytes induced by IgA1 isolated from IgA nephropathy (IgAN) patients through direct and indirect pathways. Methods: Jacalin affinity chromatography and Sephacryl S-200 molecular sieve chromatography were used to isolate IgA1 from blood of IgAN patients made as aggregated IgA1 (aIgA1). Podocytes were incubated with aIgA1 or special treated medium from mesangial cells after coincubation with aIgA1 from IgAN patients. Apoptosis of podocytes was assessed by TUNEL staining and flow cytometry. Real-time PCR was used to detect the mRNA expression of Bcl-2, Bax, Fas and Fas-L. Results: AIgA1 from IgAN patients induced more apoptosis of podocytes by both time and concentration-dependent patterns than control (30.5±5.4% vs 20.5±4.5, respectively, P<0.05). The percentage of apoptotic podocytes exposed to treated medium was higher than control (28.5±5.9 % vs 20.5±4.5%, respectively, P<0.05). The level of normalized Fas mRNA expression in podocytes exposed to aIgA1 was 2.4-fold higher than control (P<0.05), while the level in podocytes exposed with treated medium was 1.89-fold higher than control (P<0.05), and the level of normalized Bcl-2 mRNA expression in this group was 72% lower than control (P<0.05) Conclusion: IgA1 from IgAN patients may induce apoptosis of podocytes through direct and indirect pathways. IgA1 may accelerate progression of IgAN by inducing apoptosis of podocytes.
ACCESSION #
28105678

 

Related Articles

  • Evidence-based classification of glomerular pathology. Cook, Terry; Roberts, Ian S D; Feehally, John; Barratt, Jonathan // Kidney International;Nov2014, Vol. 86 Issue 5, p1059 

    A letter to the editor is presented in response to an article about the Oxford Classification of IgA Nephropathy as evidence-based classification of glomerular disease.

  • Significance of Immunostaining Data in IgA Nephropathy Patients; Current Knowledge and New Concepts. Baradaran, Azar; Nasri, Hamid // Iranian Journal of Allergy, Asthma & Immunology;Jun2014, Vol. 13 Issue 3, p218 

    A letter to the editor is presented which discusses the importance of immunostaining findings in patients with Immunoglobulin A (IgA) Nephropathy.

  • In this Issue.  // Kidney International;Aug2015, Vol. 88 Issue 2, p209 

    An introduction is presented in which the editor discusses various reports within the issue on topics including the use of ZS-9 potassium oral cation exchanger in chronic kidney disease patients, characteristics of IgA nephropathy in mice and association between kidney hypoxia and inflammation.

  • IgA nephropathy.  // Kidney International;Feb1995, Vol. 47 Issue 2, p377 

    The article profides information on various topics related immunoglobulin A nephropathy (IgAN). The main function of the IgA system is to prevent the invasion of the internal milieu. IgAN presents with painless macroscopic hematuria frequently at the time of infectious illnesses. Moreover, the...

  • Steroids in the Treatment of IgA Nephropathy to the Improvement of Renal Survival: A Systematic Review and Meta-Analysis. Yu-Hao Zhou; Li-Gong Tang; Shi-Lei Guo; Zhi-Chao Jin; Mei-Jing Wu; Jia-Jie Zang; Jin-Fang Xu; Chun-Fang Wu; Ying-Yi Qin; Qing Cai; Qing-Bin Gao; Shan-Shan Zhang; Dand-Hui Yu; Jia He // PLoS Clinical Trials;Apr2011, Vol. 8 Issue 4, Special section p1 

    Background: Studies have shown that steroids can improve kidney survival and decrease the risk of proteinuria in patients with Immunoglobulin A nephropathy, but the overall benefit of steroids in the treatment of Immunoglobulin A nephropathy remains controversial. The aim of this study was to...

  • Nutcracker phenomenon in IgA nephropathy. Imai, Naohiko; Shirai, Sayuri; Shibagaki, Yugo; Kimura, Kenjiro // Clinical Kidney Journal;Jun2014, Vol. 7 Issue 3, p325 

    The article presents a case study of a 34-year-old Japanese woman with recurrent gross hematuria. Her medical history was unremarkable and was not taking any medications. Topics include hematuria and proteinuria, nutcracker phenomenon in IgA nephropathy and percutaneous kidney biopsy. It also...

  • Persistent microhaematuria with negative or low proteinuria. Gutiérrez, Eduardo; Moreno, Juan A.; Praga, Manuel // Nefrologia;2014, Vol. 34 Issue 1, p110 

    The level of proteinuria continues to be the clinical parameter that is best related to the development of long-term renal failure in glomerular pathologies. This quantity is particularly important when we analyse the progression of patients with IgA nephropathy. As such, the natural progression...

  • Strong association between IgA nephropathy and HLA-DR4 antigen. Kashiwabara, Hidehiko; Shishido, Hideo; Tomura, Shigeo; Tuchida, Hiroki; Miyajima, Tetsuya // Kidney International;Oct1982, Vol. 22 Issue 4, p377 

    Although the etiology and pathogenesis of IgA nephropathy is not fully understood, it has been classified as an immune-complex induced glomerulonephritis caused by an immunological mechanism. Forty-two patients with primary IgA nephropathy underwent tissue typing for HLA-A, B, and DR antigens by...

  • IgM and C1q Labeling Do Not Indicate Severe Disease.  // Kidney;May/Jun99, Vol. 8 Issue 3, p105 

    Discusses the abstract of a study entitled `Immunoglobulin M and C1q mesangial labeling in IgA nephropathy,' by T.R. Welch, J. McAdams, published in the 1998 'American Journal of Kidney Diseases.'

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics