Protein voltammetry and spectroscopy: integrating approaches

Male, Louise; Marritt, Sophie J.; Berks, Ben C.; Cheesman, Myles R.; van Wonderen, Jessica H.; George, Simon J.; Butt, Julea N.
January 2008
Theoretical Chemistry Accounts: Theory, Computation, & Modeling;Jan2008, Vol. 119 Issue 1-3, p107
Academic Journal
Cyclic voltammetry readily visualizes the redox properties of many proteins. Net electron exchange between the protein and an electrode produces an electrical current that simultaneously quantitates and characterizes the underlying redox event(s). However, no direct information regarding the molecular origin, or consequences, of electron transfer is available. Integrating voltammetric and spectroscopic methods is one route to a more ‘holistic’ description of protein electron transfer. Here, we illustrate this approach with spectroelectrochemical studies of Rhodovulum sulfidophilum cytochrome c 2 and Escherichia coli cytochrome bd that employ electronic absorbance, infra-red and magnetic circular dichroism spectroscopies.


Related Articles

  • A Measurement System For Circular Dichroism In Soft X-ray Absorption Using Helicity Switching By Twin Helical Undulators. Muro, T.; Saitoh, Y.; Kimura, H.; Matsushita, T.; Nakatani, T.; Takeuchi, M.; Hirono, T.; Kudo, T.; Nakamura, T.; Wakita, T.; Kkobayashi, K.; Hara, T.; Shirasawa, K.; Kitamura, H.; Warwick, T. // AIP Conference Proceedings;2004, Vol. 705 Issue 1, p1051 

    A measurement system for circular dichroism (CD) of soft X-ray absorption has been developed using helicity switching of circularly polarized radiation by twin helical undulators at BL25SU of SPring-8. Difference of horizontal spot positions between the negative- and positive-helicity light at...

  • Protein GB1 Folding and Assembly from Structural Elements. Bauer, Mikael C.; Wei-Feng Xue; Linse, Sara // International Journal of Molecular Sciences;Apr2009, Vol. 10 Issue 4, p1552 

    Folding of the Protein G B1 domain (PGB1) shifts with increasing salt concentration from a cooperative assembly of inherently unstructured subdomains to an assembly of partly pre-folded structures. The salt-dependence of pre-folding contributes to the stability minimum observed at physiological...

  • Characterizing vibrational motion beyond internal coordinates. Hug, Werner; Fedorovsky, Maxim // Theoretical Chemistry Accounts: Theory, Computation, & Modeling;Jan2008, Vol. 119 Issue 1-3, p113 

    We present a procedure for the decomposition of the normal modes of a composite system, including its rotations and translations, into those of fragments. The method permits—by the cross-contraction of dyads of mass-weighted displacement vectors, without recourse to valence...

  • Synthesis, Chiral Resolution, and Absolute Configuration of Functionalized Tr�ger's Base Derivatives: Part II. Benkh�user-Schunk, Christian; Wezisla, Boris; Urbahn, Kirstin; Kiehne, Ulf; Daniels, J�rg; Schnakenburg, Gregor; Neese, Frank; L�tzen, Arne // ChemPlusChem;May2012, Vol. 77 Issue 5, p396 

    Seven racemic derivatives of Tr�ger's base-the 1,7-dibromo-substituted derivative 3, the 2,8-dibromo-substituted derivative 4, the 2,8-diiodo-substituted derivative 5, the 3,9-diiodo-substituted derivative 6, the 4,10-dibromo-substituted derivative 7, its singly debrominated analogue 8, and...

  • Determination of Absolute Configuration.  // Spectroscopy;Mar2006 Supplement, Vol. 21, p19 

    The article shows an example of determination of absolute configuration (AC) for the polyphenolic binaphpthyl dialdehyde, gossypol. It discusses the ability of gossypol to inhibit maturation in human sperm and its potential as an antineoplastic agent. The article details the use of vibrational...

  • Probing chiral solute-water hydrogen bonding networks by chirality transfer effects: A vibrational circular dichroism study of glycidol in water. Yang, Guochun; Xu, Yunjie // Journal of Chemical Physics;4/28/2009, Vol. 130 Issue 16, p164506 

    Vibrational absorption (VA) and vibrational circular dichroism (VCD) spectra of (S)-(-)-glycidol were measured in water with a concentration of 6.0M in the 1000–1750 cm-1 region. Prominent and complex VCD spectral features were detected at the water bending vibrational region. Our...

  • Reduction of linear birefringence in vibrational circular dichroism measurement: use of a rotating half-wave plate. Xiaolin Cao; Dukor, Rina K.; Nafie, Laurence A. // Theoretical Chemistry Accounts: Theory, Computation, & Modeling;Jan2008, Vol. 119 Issue 1-3, p69 

    A new method has been developed to suppress the effects of linear birefringence (LB) associated with sample cells in the measurement of vibrational circular dichroism (VCD) spectra by adding a rotating half-wave plate (RHWP) to the optical train. The method functions in combination with the...

  • Effects of High Hydrostatic Pressure on Escherichia coli Ultrastructure, Membrane Integrity and Molecular Composition as Assessed by FTIR Spectroscopy and Microscopic Imaging Techniques. Prieto-Calvo, María; Prieto, Miguel; López, Mercedes; Alvarez-Ordóñez, Avelino // Molecules;Dec2014, Vol. 19 Issue 12, p21310 

    High hydrostatic pressure (HHP) is a novel food processing technology that is considered as an attractive alternative to conventional heat treatments for the preservation of foods, due to its lethal effects on pathogenic and spoilage microorganisms, while causing minor effects on food quality...

  • Structural and spectroscopic studies of a model for catechol oxidase. Smith, Sarah J.; Noble, Christopher J.; Palmer, Randahl C.; Hanson, Graeme R.; Schenk, Gerhard; Gahan, Lawrence R.; Riley, Mark J. // Journal of Biological Inorganic Chemistry;Apr2008, Vol. 13 Issue 4, p499 

    A binuclear copper complex, [Cu2(BPMP)(OAc)2][ClO4]·H2O, has been prepared using the binucleating ligand 2,6-bis[bis(pyridin-2-ylmethylamino)methyl]-4-methylphenol (H-BPMP). The X-ray crystal structure reveals the copper centers to have a five-coordinate square pyramidal geometry, with the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics