In vitro translation with [34S]-labeled methionine, selenomethionine, and telluromethionine

Ogra, Yasumitsu; Kitaguchi, Takashi; Suzuki, Noriyuki; Suzuki, Kazuo T.
January 2008
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 1, p45
Academic Journal
Heteroisotope and heteroatom tagging with [34S]-enriched methionine (Met), selenomethionine (SeMet), and telluromethionine (TeMet) was applied to in vitro translation. Green fluorescent protein (GFP) and JNK stimulatory phosphatase-1 (JSP-1) genes were translated with wheat germ extract (WGE) in the presence of Met derivatives. GFPs containing Met derivatives were subjected to HPLC coupled with treble detection, i.e., a photodiode array detector, a fluorescence detector, and an inductively coupled plasma mass spectrometer (ICP-MS). The activities of JSP-1-containing Met derivatives were also measured. GFP and JSP-1 containing [34S]-Met and SeMet showed comparable fluorescence intensities and enzyme activities to those containing naturally occurring Met. TeMet was unstable and decomposed in WGE, whereas SeMet was stable throughout the experimental period. Thus, although Te was the most sensitive to ICP-MS detection among S, Se, and Te, TeMet was less incorporated into the proteins than Met and SeMet. Finally, the potential of heteroisotope and heteroatom tagging of desired proteins in in vitro translation followed by ICP-MS detection was discussed. [Figure not available: see fulltext.]


Related Articles

  • methionine.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1457 

    A definition of the medical term "methionine," which refers to a sulfur containing essential amino acid, is presented.

  • Analysis of the Present Status of the Methionine Market. Jianhua, Guo // China Chemical Reporter;7/16/2004, Vol. 15 Issue 20, p20 

    Analyzes the status of the methionine market. Development in the production of methionine; Impact of the development of the international feed industry on methionine; Consumption of methionine in China.

  • Methionine requirement and cell-mediated immunity in chicks. Shini, S.; Li, X.; Bryden, W. L. // Asia Pacific Journal of Clinical Nutrition;2005 Supplement, Vol. 14, pS123 

    Background -- In addition to the requirement for growth, methionine has been shown to have beneficial effects on the immune status of animals. Objective -- To determine the methionine requirements for growth and the cellular immune response in broiler chicks. Design -- One-day-old Ross broiler...

  • Biologically active organophosphorous analogues of methionine in reactions catalyzed by L-methionine γ-lyase. Alferov, K. V.; Faleev, N. G.; Demidkina, T. V.; Khurs, E. N.; Morozova, E. A.; Khomutov, R. M. // Doklady Biochemistry & Biophysics;Mar2006, Vol. 407 Issue 1, p102 

    The article examines the interaction of organophosphorus analogues of methionine with pyridoxal-5'-phosphate-dependent L-methionine γ-lyase from bacteria of the genus Citrobacter. The study determines the set of properties characterizing the uniqueness of methionine as a biologically active...

  • Importance of the trans-sulfuration pathway in cancer prevention and promotion. Joemerson Rosado; Mirian Salvador; Diego Bonatto // Molecular & Cellular Biochemistry;Jul2007, Vol. 301 Issue 1/2, p1 

    Abstract  The trans-sulfuration pathway is a biochemical mechanism that links methionine metabolism to the biosynthesis of cellular redox-controlling molecules, like cysteine, glutathione, and taurine. While there is some knowledge about the metabolic intermediates and enzymes...

  • Lack of Significant Effects of Methionine Loading on Endothelial Function in Elderly Volunteers Hart, Simon R.; Mangoni, Arduino A.; Swift, Cameron G.; Jackson, Stephen H.D. // Heart, Lung & Circulation;Dec2006, Vol. 15 Issue 6, p358 

    Objective: To test the hypothesis that an acute increase in plasma homocysteine concentration (Hcy) produced by methionine loading is associated with an acute decrease in brachial artery blood flow measured by flow-mediated dilatation (FMD) using forearm plesthysmography. Design: A double-blind,...

  • Influence of Impaired Liver Methionine Metabolism on the Development of Vascular Disease and Inflammation. Avila, M. A.; Berasain, C.; Prieto, J.; Mato, J. M.; Garc�a-Trevijano, E. R.; Corrales, F. J. // Current Medicinal Chemistry - Cardiovascular & Hematological Age;Jul2005, Vol. 3 Issue 3, p267 

    Methionine (Met) metabolism involves the sequential formation of S-adenosylmethionine (SAM, the main biological methyl donor), S-adenosylhomocysteine (SAH) and homocysteine (Hcy). Hcy can be remethylated to Met or catabolized through the trans-sulfuration pathway. In mammals, as much as 48% of...

  • Comparison of volatile sulphur compound production by cheese-ripening yeasts from methionine and methionine�cysteine mixtures. L�pez del Castillo-Lozano, M.; Delile, A.; Spinnler, H. E.; Bonnarme, P.; Landaud, S. // Applied Microbiology & Biotechnology;Jul2007, Vol. 75 Issue 6, p1447 

    Production of volatile sulphur compounds (VSC) was assessed in culture media supplemented with l-methionine or l-methionine/ l-cysteine mixtures, using five cheese-ripening yeasts: Debaryomyces hansenii DH47(8), Kluyveromyces lactis KL640, Geotrichum candidum GC77, Yarrowia lipolytica YL200 and...

  • No Correlation between Plasma Hcy Concentration and SNP in Methionine Synthase Gene.  // Fertility Weekly;8/4/2008, p10 

    The article presents a study on methionine synthase gene on plasma homocysteine (Hcy) levels conducted by M. Palep-Singh and associates in Great Britain. The presence of a single nucleotide polymorphism (SNP) in the methionine synthase gene does not affect plasma Hcy levels to women with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics