TITLE

Surface electric field manipulation of the adsorption kinetics and biocatalytic properties of cytochrome c on a 3D macroporous Au electrode

AUTHOR(S)
Yan-Yan Song; Ying Li; Chen Yang; Xing-Hua Xia
PUB. DATE
January 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 1, p333
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Upon adsorbing on a solid-state substrate, water-soluble proteins are prone to denaturation and deterioration of their functions due to the conformation change. The surface electric field of a conductive substrate is one of the important factors that influence the character of adsorbed proteins. In this work, a 3D macroporous gold electrode has been prepared and served as the working electrode to study the influence of surface electric field on the adsorption kinetics and conformation of the adsorbed cytochrome c (cyt-c) with the help of electrochemical, in situ electrochemical IR spectroscopic, atomic force microscopic, and contact angle measurements. The external electric field creates excess surface charge which can manipulate the adsorption rate of proteins on the substrate by the enhanced electrostatic interactions between the electrode and protein patches by coupling with complementary charges. The amount of immobilized cyt-c with electrochemical activity on the 3D macroporous gold electrode showed a minimum at potential of zero charge (PZC) and it increased with increasing net excess surface charge. Higher electric field could influence the conformation and the corresponding properties such as direct electrochemistry, bioactivity, and surface character of the adsorbed cyt-c molecules. However, high external electric field leads to damage of the protein secondary structure. This study provides fundamentals for the fabrication of biomolecular devices, biosensors, and biofuel cells through electrostatic interactions. [Figure not available: see fulltext.]
ACCESSION #
28000667

 

Related Articles

  • Comparison of spatial resolution in internal and external electro-optic probing. H . B. Zhang; R. Wang; H. Yang; D.M. Zhang; M.B. Y; G . Q . Wang; Z . C . Ma // Optical & Quantum Electronics;Jun2003, Vol. 35 Issue 8, p757 

    A new method, based on zero point of longitudinal electric field, was used to determine the spatial resolution of internal and external electro-optic (EO) probing systems. An interdigital structure electrode was fabricated on GaAs substrate to examine the spatial resolution of internal and...

  • The Velocity of Streamer Propagation toward Anode and Cathode in He, Xe, N2 , and SF6. Yakovlenko, S. I. // Technical Physics Letters;May2004, Vol. 30 Issue 5, p354 

    A simple mechanism of ionization spreading in a dense gas, based on the background electron multiplication wave in an inhomogeneous electric field, is considered. This process is independent of the sign of the field projection onto the direction of propagation. An analytical expression for the...

  • Equilibrium Configurations of the Charged Surface of a Conducting Liquid at a Finite Interelectrode Distance. Zubarev, N. M.; Zubareva, O.V. // Technical Physics Letters;Nov2004, Vol. 30 Issue 11, p905 

    The problem of determining equilibrium configurations of the free surface of a conducting liquid is considered with allowance for a finite interelectrode distance. An analogy is established between this electrostatic problem and that of finding the profile of a progressive capillary wave on the...

  • The elimination of interface charge density singularity in single layer organic semiconductor structures. Cvikl, B. // Journal of Applied Physics;Aug2011, Vol. 110 Issue 3, p033723 

    The arguments are presented that the assumption of a zero electric field at the charge carrier injection electrode/organic interface, as assumed by the current-voltage model of shallow traps occupying a single energy level within the organic semiconductor charge transport band as well as by the...

  • Spatially periodical poling of silica glass. Sokolov, K.; Melehin, V.; Petrov, M.; Zhurikhina, V.; Lipovskii, A. // Journal of Applied Physics;May2012, Vol. 111 Issue 10, p104307 

    The process of poling of silica glass using periodical anodic electrode, or, the same, electric field imprinting of periodical structure in silica glass is modeled. We studied the influence of the electrode periodicity and the magnitude of applied voltage on the thickness of poled (charge...

  • Spatial Macroscopic Structure of a Pulsed Microsecond Discharge in Barrier�Metal Gaps with a Nonuniform Electric Field Distribution. Buranov, S. N.; Gorokhov, V. V.; Karelin, V. I.; Tren'kin, A. A. // Technical Physics;Sep2004, Vol. 49 Issue 9, p1227 

    Electric and spatial characteristics of a pulse-periodic microsecond barrier discharge are investigated in different geometries�triangular prism, plate, and corrugated electrode�that are in contact with a dielectric plate and form a dihedral angle with it. It is established that, in...

  • Colloidal electroconvection in a thin horizontal cell. I. Microscopic cooperative patterns at low voltage. Han, Yilong; Grier, David G. // Journal of Chemical Physics;4/22/2005, Vol. 122 Issue 16, p164701 

    Applying an electric field to an aqueous colloidal dispersion establishes a complex interplay of forces among the highly mobile simple ions, the more highly charged but less mobile colloidal spheres, and the surrounding water. This interplay can induce a wide variety of visually striking...

  • Reversible electrochromic effect accompanying domain-inversion in LiNbO3:Ru:Fe crystals. Qingxin Xi; De'an Liu; Ya'nan Zhi; Zhu Luan; Liren Liu // Applied Physics Letters;9/19/2005, Vol. 87 Issue 12, p121103 

    A reversible electrochromic effect during the electrical poling process in LiNbO3:Ru:Fe crystals at room temperature is observed. In electrode area, both electrochromism and domain-inversion occur mutually and electrochromism is reversible during back-switch poling, which are experimentally...

  • Gas Adsorption in Electric Field. Golovanchikov, A. B.; Efremov, M. Yu.; Safonov, E. V. // Theoretical Foundations of Chemical Engineering;Sep/Oct2003, Vol. 37 Issue 5, p514 

    Describes the process of gas adsorption in electric field. Equation that describes the dependence of the contact angle; Effect of an electric field on the adsorption ability of a solid adsorbent; Determination of the weight of adsorbed water.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics