TITLE

High temperature and temperature programmed HPLC: possibilities and limitations

AUTHOR(S)
Vanhoenacker, Gerd; Sandra, Pat
PUB. DATE
January 2008
SOURCE
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 1, p245
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The article examines the use of elevated temperature and temperature programming in high-performance liquid chromatography (HPLC). It tackles in detail the many advantages of increasing the column temperature. In nearly all reversed-phase separations, a rise in temperature will also cause a decrease in retention. High linear velocities need to be used in order to fully exploit the potential of sub-two-micron columns. Also by increasing the temperature, the amount of organic solvent in the mobile phase can be reduced to maintain retention.
ACCESSION #
28000655

 

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