High temperature and temperature programmed HPLC: possibilities and limitations

Vanhoenacker, Gerd; Sandra, Pat
January 2008
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 1, p245
Academic Journal
The article examines the use of elevated temperature and temperature programming in high-performance liquid chromatography (HPLC). It tackles in detail the many advantages of increasing the column temperature. In nearly all reversed-phase separations, a rise in temperature will also cause a decrease in retention. High linear velocities need to be used in order to fully exploit the potential of sub-two-micron columns. Also by increasing the temperature, the amount of organic solvent in the mobile phase can be reduced to maintain retention.


Related Articles

  • Development of a Stability-Indicating RP-HPLC Method for the Determination of Rupatadine and its Degradation Products in Solid Oral Dosage Form. TRIVEDI, Harshal Kanubhai; PATEL, Mukesh C. // Scientia Pharmaceutica;2012, Vol. 80 Issue 4, p889 

    A simple, sensitive, and reproducible reversed-phase high-performance liquid chromatography (RP-HPLC) method, coupled with a photodiode array detector, was developed for the determination of rupatadine (RUPA) and its related substances in pharmaceutical dosage forms. Chromatographic separation...

  • Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules. Zhu, Cuiru; Goodall, David M.; Wren, Stephen A. C. // LC-GC North America;Jan2005, Vol. 23 Issue 1, p54 

    Evaluates the use of elevated and high temperatures in high performance liquid chromatography (HPLC). Discussion of generic issues of temperature dependence of retention and plate height; Significance of the use of elevated temperature in allowing good chromatographic efficiency; Comparison of...

  • Stationary Phases for LC Separations at Elevated Temperatures. Yu Yang // LC-GC North America;Apr2006 Supplement 4, Vol. 24, p53 

    With the advent of column materials that can be used effectively at temperatures well over 100 �C, high temperature liquid chromatography (HTLC) separations have gained more attention in the last few years. Advantages of HTLC include fast analysis time, better selectivity, improved column...

  • The Misunderstood Laboratory Solvent: Reagent Water for HPLC. Mabic, Stéphane; Regmault, Cecilia; Krol, Jim // LC-GC North America;Jan2005, Vol. 23 Issue 1, p74 

    Discusses the usefulness of reagent water in all aspects of liquid chromatography technology. Utility of the solvent from preparation of mobile phase to preparation of standards; Lack of characterization of the total organic carbon (TOC) content of the solvent; Effect of TOC on the performance...

  • The Power of Mobile Phase Strength. Dolan, John W. // LC-GC Europe;Jul2006, Vol. 19 Issue 7, p396 

    The article discusses on the usage of solvent strength in predicting changes in the peak movement for reversed-phase liquid chromatography. The regular movement of peaks and the rule of three helps in finding conditions that give reasonable retention. Finding conditions intermediate to...

  • Solvent Conservation. Dolan, John W. // LC-GC North America;Mar2007, Vol. 25 Issue 3, p268 

    The author reflects on ways for reducing solvent consumption for liquid chromatography (LC) methods. He says that the environmental considerations are the only real justification for reducing solvent usage for LC processes. He reckons that the best strategy in conserving mobile phase volume is...

  • Too Many Changes. Dolan, John W. // LC-GC North America;Aug2009, Vol. 27 Issue 8, p606 

    The article discusses the impact of certain problems that affect the liquid chromatography (LC) method. It mentions the isocratic method which is used to save acetonitrile, and used a C18 chromatography column that is operated at two milliliters per minute at 30° centigrade. It states that it...

  • But I Can't Change the Method. Dolan, John W. // LC-GC Asia Pacific;Nov2004, Vol. 7 Issue 4, p12 

    Presents several tips for transferring a validated liquid chromatography method from one laboratory to another. Impact of the stronger injection solvent than the mobile phase on the peak shape of the sample using the method; Significance of checking the retention factors of the peaks in every...

  • Isolation and Purification of Six Polymethoxyflavones from Pericarpium citri Reticulatae Viride by High-speed Counter-current Chromatography. YU Bo; PENG Ai-yi; QI Xin; QU Xue-wei; LI Hui; YANG Hong // Natural Product Research & Development;Jun2010, Vol. 22 Issue 3, p425 

    Six Polymethoxyflavones were prepared, isolated and purified from Pericarpium cirri Reticulatae Viride by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (4:6:4:6,v/v/v/v) was used. With in 460 minuets, 3.5 mg of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics