Novel chromatography techniques for high-throughput analysis of proteomes

Yuan, Huiming; Zhang, Lihua; Liang, Zhen; Zhang, Yukui
January 2008
Analytical & Bioanalytical Chemistry;Jan2008, Vol. 390 Issue 1, p273
Academic Journal
The article deals with the advances in the development of novel chromatography techniques for the high-throughput analysis of proteomes. It is beneficial to perform HPLC at elevated temperatures in a high-throughput two-dimensional high performance liquid chromatography (2D-HPLC) platform, because the resulting decrease in the viscosity of the mobile phase permits the employment of packing materials with small particle sizes, even at high flow rates. It also tackles the important role of capillary electrophoresis in the fast analysis of biomolecules.


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