TITLE

Rat epididymal epithelial cells and 17β-estradiol synthesis under hCG stimulation in vitro

AUTHOR(S)
Świder-Al-Amawi, Małgorzata; Marchlewicz, Mariola; Kolasa, Agnieszka; Wenda-Różewicka, Lidia; Wiszniewska, Barbara
PUB. DATE
December 2007
SOURCE
Folia Histochemica et Cytobiologica;2007, Vol. 45 Issue 3, p255
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Epithelial cells of human and animal epididymis display features of steroidogenic cells. Rat epididymal epithelial cells in vitro produce androgens which are converted to 17β-estradiol, and released into the medium. The regulation of the epididymal steroidogenesis is not fully understood but it could be expected that it remains under LH influence. In previous study we observed that the morphology of rat epididymal epithelial cells in vitro was affected by hCG and the increase of amount of lipid droplets, glycogen and PAS-positive substances was observed. The present studies show the organelles which take part in synthesis of steroids in rat epididymal epithelial cells in vitro and the effect of hCG on E2 synthesis. The cells were cultured in the medium with/without DHT and without DHT in supplementation with hCG. After hCG stimulation the amount of an active mitochondria were increased when compared to the amount of mitochondria in the epididymal epithelial cells cultured without DHT. Ultrastructure of the cells was similar to the cells cultured with DHT, while the cytoplasm of the cells cultured without DHT was disorganized. The synthesis of 17β-estradiol was stimulated by hCG, that exerted its effect through LH/hCG receptors, localized in the epididymal epithelial cells.
ACCESSION #
27680728

 

Related Articles

  • IMMUNOHISTOMORPHOMETRIC FEATURES OF ACTH CELLS IN JUVENILE RATS AFTER TREATMENT WITH ESTRADIOL OR HUMAN CHORIONIC GONADOTROPIN.  // Journal of Medical Biochemistry;Mar2012, Vol. 31 Issue 1, p34 

    The article presents a study which examined the morphometric parameters of immunohistochemically labeled ACTH cells in juvenile female rat pituitaries after treatment of estradiol or human chorionic gonadotropin. The researchers assessed the secretory activity of hormone producing cells in the...

  • Human Endometrial CD98 Is Essential for Blastocyst Adhesion. Domínguez, Francisco; Simón, Carlos; Quiñonero, Alicia; Ramírez, Miguel Ángel; González-Muñoz, Elena; Burghardt, Hans; Cervero, Ana; Martínez, Sebastián; Pellicer, Antonio; Palacín, Manuel; Sánchez-Madrid, Francisco; Yáñez-Mó, María // PLoS ONE;2010, Vol. 5 Issue 10, p1 

    Background: Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans. Methods and Principal Findings: Using two...

  • Human Chorionic Gonadotropin Combined with Progesterone for Luteal Support Improves Pregnancy Rate in Patients with Low Late-Midluteal Estradiol Levels in IVF Cycles. Fujimoto, Akihisa; Osuga, Yutaka; Fujiwara, Toshihiro; Yano, Tetsu; Tsutsumi, Osamu; Momoeda, Mikio; Kugu, Koji; Koga, Kaori; Morita, Yutaka; Wada, Osamu; Taketani, Yuji // Journal of Assisted Reproduction & Genetics;Dec2002, Vol. 19 Issue 12, p550 

    Examines the effects of combining progesterone and human chorionic gonadotropin (hCG) on pregnancy rate of patients with low late-midluteal estradiol level. Impact of hCG on late-midluteal estradiol level; Comparison on pregnancy outcome in patients with different late-midluteal estradiol level;...

  • hCG regulates human endometrial epithelial cell adhesion through L-selectin ligand, MECA- 79. Kabir-salmani, M.; Nejatbakhsh, R.; Hosseini, A.; Dimitriadis, E. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p32 

    Introduction: Molecular interactions at the human embryo-endometrium interface play critical roles during embryo implantation. L-selectin and its carbohydrate ligand are important in the human embryo-endometrial apposition stage and blastocyst-derived hCG plays a significant roles during...

  • The Direct Effect of Hepatic Peroxisome Proliferators on Rat Leydig Cell Function in Vitro1. LIU, RAYMOND C. M.; HAHN, CINDY; HURTT, MARK E. // Fundamental & Applied Toxicology;1996, Vol. 30 Issue 1, p102 

    A review of the literature indicates that some compounds which produce hepatic peroxisome proliferation in rats also appear to produce Leydig cell adenomas, and some also affect the serum concentrations of testosterone and estradiol. Previous studies with the peroxisome proliferator ammonium...

  • Immunohistomorphometric Characteristics Of Pituitary GH Cells In Infant And Peripubertal Female Rats After Treatment With Estradiol Or Human Chorionic Gonadotropin. Ristic, Natasa; Ajdzanovic, Vladimir; Trifunovic, Svetlana; Tanic, Nasta; Bujisic, Nada; Milosevic, Verica // Serbian Journal of Experimental & Clinical Research;Dec2015, Vol. 16 Issue 4, p281 

    The effects of estradiol-dipropionate (EDP) or human chorionic gonadotropin (hCG) on immunohistomorphometric characteristics of pituitary GH cells in infant and peripubertal female rats were investigated. The first group of females received five injections of EDP (0.25 mg/kg b.w.) during the...

  • Reproductive Hormones in the Regulation of Apoptosis of Neutrophils. Shirshev, S. V.; Kuklina, E. M.; Yarilin, A. A. // Biochemistry (00062979);Jun2003, Vol. 68 Issue 6, p688 

    The ability of main reproductive hormones such as chorionic gonadotropin (CG), estradiol, and progesterone to regulate apoptosis of human neutrophils was studied. The hormones were studied separately and in physiological combinations specific for different trimesters of pregnancy. A low dose of...

  • The Role of Estradiol in Gonadotropin-Dependent Regulation of Neutrophil Function. Shirshev, S. V.; Kuklina, E. M. // Doklady Biochemistry & Biophysics;Nov/Dec2000, Vol. 375 Issue 1-6, p218 

    Presents a study on the role of estradiol in the chorionic gonadotropin-dependent regulation of neutrophil activity. Factors which regulate neutrophil function during pregnancy; Indices determined in evaluating the phagocytic activity of neutrophils; Conclusion of the research.

  • Modulation of human cytotrophoblastic leptin secretion by interleukin-1α and 17β-oestradiol and its effect on HCG secretion. Chardonnens, D.; Cameo, P.; Aubert, M.L.; Pralong, F.P.; Islami, D.; Campana, A.; Gaillard, R.C.; Bischof, P. // MHR: Basic Science of Reproductive Medicine;Nov99, Vol. 5 Issue 11, p1077 

    To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17β-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics