TITLE

Characterization and application of quantum dot nanocrystal—monoclonal antibody conjugates for the determination of sulfamethazine in milk by fluoroimmunoassay

AUTHOR(S)
Jianzhong Shen; Fei Xu; Haiyang Jiang; Zhanhui Wang; Jing Tong; Pengju Guo; Shuangyang Ding
PUB. DATE
December 2007
SOURCE
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2243
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Quantum dot (Qdot) nanocrystals have been increasingly used as fluorescence labels in fluoroimmunoassays recently because of their excellent optical characteristics. In this paper, a new monoclonal antibody (MAb) against sulfamethazine (SMZ) was successfully produced and linked to Qdot nanocrystals by covalent coupling. The Qdot–MAb conjugates were characterized by SDS-PAGE and high-performance capillary electrophoresis (HPCE). An enzyme-linked immunosorbent assay (ELISA) method was utilized to evaluate the antigen–antibody binding affinity and then a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) for the detection of SMZ in milk by using Qdots as fluorescent labels was evaluated. The results showed that the 50% inhibition values (IC50) of the cFLISA were 4.3 ng/mL in milk and 5.2 ng/mL in PBS, and the limits of detection (LODs) were 0.6 ng/mL in milk and 0.4 ng/mL in PBS, respectively. The recoveries of SMZ from spiked milk samples at levels of 10–100 ng/mL ranged from 94 to 106%, with coefficients of variation (CVs) of 2.1–9.2%. [Figure not available: see fulltext.]
ACCESSION #
27657812

 

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