TITLE

Characteristics of a liposome immunoassay on a poly(methyl methacrylate) surface

AUTHOR(S)
Sang Youn Hwang; Yoichi Kumada; Gi Hoon Seong; Jaebum Choo; Shigeo Katoh; Eun Kyu Lee
PUB. DATE
December 2007
SOURCE
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2251
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Liposome immunoassay (LIA) is based on enzyme immunoassay (EIA) but the detection sensitivity could be significantly enhanced by using antibody-coupled immunoliposomes encapsulating HRP (horse radish peroxidase). Here, we applied LIA to non-porous poly(methyl methacrylate) (PMMA) and polystyrene (PS) surfaces to compare its detection sensitivity with that of EIA, using rabbit IgG (a ligand molecule) and anti-rabbit IgG antibody (a capture molecule) as the model system. LIA developed much stronger color signals than EIA, especially at a lower concentration range (< ca. 1 μg mL−1). PMMA showed higher affinity toward rabbit IgG than the PS surface, and the anti-rabbit IgG antibody adsorbed on PMMA was more stable than that on PS. Furthermore, the effects of spot volume and antibody concentration on the signal density were analyzed. The signal density increased as the antibody concentration increased, but it was not significantly affected by the spot volume (2.5–20 μL). In conclusion, LIA on PMMA as a solid support is a very useful, highly sensitive microarray detection system.
ACCESSION #
27657808

 

Related Articles

  • Computational analysis of non-covalent polymer-protein interactions governing antibody orientation. Farris, Leslie; McDonald, Melisenda // Analytical & Bioanalytical Chemistry;Mar2012, Vol. 402 Issue 4, p1731 

    The ALYGNSA is an affinity-based antibody orientation system produced through the interaction of the polymer poly(methyl methacrylate) (PMMA) and recombinant protein G (rProG), a streptococcal protein. This improved orientation suggests a specific non-covalent attachment of the rProG to PMMA...

  • Some Potential Pitfalls of Immunoassays. Hansen, Keith A. // South Dakota Medicine;May2012, p174 

    The author discusses the potential pitfalls of immunoassays. According to the author, the development of immunoassays has been dependent on the highly specific interaction between antibody and antigen. The author notes that inappropriate results can cause significant clinical decisions that can...

  • Electrochemoluminescent Immunoassay (ECLIA). Vohr, Hans-Werner // Encyclopedic Reference of Immunotoxicology;2005, p222 

    The article presents an encyclopedia entry for electrochemoluminescent immunoassay (ECLIA). It is an immunoassay in which a light-generating chemical reaction occurs during binding between a ruthenium tri-bispyridine-labeled analyte, captured in an antigen-antibody-bead complex and tripopylamine.

  • Evaluation of the Dade Behring Syva® EMIT 2000 tacrolimus assay on the Bayer Advia 1650. Stephen, DWS; Rooke, P; Clark, S; Coutts, M; Crowe, L; Docherty, D // Annals of Clinical Biochemistry;Nov2003, Vol. 40 Issue 6, p697 

    Background: The Dade Behring Syva® EMIT (enzyme multiplied immunoassay technique) method for the measurement of tacrolimus in whole blood was evaluated against the Abbott IMx® microparticle enzyme immunoassay (MEIA) method. EMIT measures tacrolimus colorimetrically, whereas MEIA measures...

  • Determination of Alachlor in food products by a fluorescence polarization immunoassay. Nartova, Yu. V.; Ermolaeva, T. N.; Fleisher, M. R.; Abuknesha, R.; Eremin, S. A. // Journal of Analytical Chemistry;May2008, Vol. 63 Issue 5, p499 

    A procedure for the determination of Alachlor in liquid media using a fluorescence polarization immunoassay (FPIA) was developed. The effects of the structure and purification degree of a tracer (analyte with a fluorescent label) on the analytical signals, sensitivity, and selectivity in the...

  • Direct electrochemical immunoassay based on a silica nanoparticles/sol—gel composite architecture for encapsulation of immunoconjugate. Fu-Chang Wang; Ruo Yuan; Ya-Qin Chai // Applied Microbiology & Biotechnology;Sep2006, Vol. 72 Issue 4, p671 

    A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol–gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this...

  • Immunoassays. Herzyk, Danuta J.; Vohr, Hans-Werner // Encyclopedic Reference of Immunotoxicology;2005, p325 

    A definition of the term "immunoassays" is presented. The term refers to methods applied in analyses of proteins also known as macromolecules. lmmunoassays as bioanalytical methods are based on formation of the specific antigen-antibody complex for detecting the presence and/or quantitation of...

  • Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads. Lates, Vasilica; Yang, Cheng; Popescu, Ionel; Marty, Jean-Louis // Analytical & Bioanalytical Chemistry;Jun2012, Vol. 402 Issue 9, p2861 

    We report here the development of a new assay for the detection of ochratoxin A (OTA) based on the use of its dechlorinated analogue, ochratoxin B (OTB), in a displacement immunoassay. OTB was immobilised on controlled-pore glass beads followed by the binding of anti-OTA antibody, with anti-IgG...

  • Direct electrochemical immunoassay based on a silica nanoparticles/sol—gel composite architecture for encapsulation of immunoconjugate. Fu-Chang Wang; Ruo Yuan; Ya-Qin Chai // Applied Microbiology & Biotechnology;Sep2006, Vol. 72 Issue 4, p671 

    A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol–gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics