Evaluation of the thermal history of bovine milk from the lactosylation of whey proteins: an investigation by liquid chromatography—electrospray ionization mass spectrometry

Losito, Ilario; Carbonara, Teresa; Monaci, Linda; Palmisano, Francesco
December 2007
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2065
Academic Journal
Reversed-phase high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (RP-HPLC–ESI-MS) has been used for analysis of the native and lactosylated forms of the main whey proteins, α-lactalbumin and β-lactoglobulins A and B, in commercial bovine milk samples after different thermal treatment (pasteurisation and ultra high-temperature, UHT, treatment), of different lipid content, and of different brands, to find markers of the thermal history of the milk. A new quantification strategy was developed, based on peak-area integration after multiple ion current extraction and considering all the ions detectable in the multi-charge ESI mass spectrum for each type of protein. Validation of the procedure for native forms was first accomplished by calibration with model solutions. Linearity was always good. Sensitivity was different for α-lactalbumin and β-lactoglobulins; the signal was stronger for the latter with only a slight difference between variants A and B of β-lactoglobulins. Application of the quantification approach to pasteurised and UHT milk samples showed that the distributions of the three proteins and of their three main forms (native, and mono and bi-lactosylated) in whey extracts can be used as statistically robust discriminatory properties for recognition of commercial thermal treatment of milk.


Related Articles

  • Argon mediated electron impact ionization of Arn(CH3OH)m heteroclusters. Vaidyanathan, Gopal; Coolbaugh, M. Todd; Peifer, William R.; Garvey, James F. // Journal of Chemical Physics;2/1/1991, Vol. 94 Issue 3, p1850 

    In this paper we have contrasted electron impact cluster mass spectra of a methanol/argon expansion with a methanol/helium expansion and have measured appearance potentials for several of the prominent ions in each case. We have observed a number of ionic species in the argon expansion (i.e.,...

  • The ionization potentials of CH4 and CD4. Berkowitz, J.; Greene, J. P.; Cho, H.; Ruscic, B. // Journal of Chemical Physics;1/15/1987, Vol. 86 Issue 2, p674 

    The adiabatic ionization potential of CD4 is measured by photoionization mass spectrometry to be 12.658±0.015 eV, which is 0.05±0.02 eV higher than that of CH4. The difference is attributed to zero point energy differences, rather than different Jahn–Teller stabilization energies.

  • Application of an Enzyme-Linked Immunosorbent Assay (ELISA) to Determine Deltamethrin Residues in Milk. Lee, H.-J.; Watanabe, T.; Gee, S. J.; Hammock, B. D. // Bulletin of Environmental Contamination & Toxicology;Jul2003, Vol. 71 Issue 1, p0014 

    The article cites a study where enzyme-linked immunoassay for deltamethrin was applied to the detection of deltamethrin in milk. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) was used to identify deltamethrin in milk samples. The LC-MS-MS system consisted of a Micromass Quattro...

  • Spectroscopy of AlAr and AlKr from 31 000 cm-1 to the ionization limit. Heidecke, Scott A.; Fu, Zhenwen; Colt, John R.; Morse, Michael D. // Journal of Chemical Physics;8/1/1992, Vol. 97 Issue 3, p1692 

    Spectra of jet-cooled AlAr and AlKr have been collected over the range from 31 000 cm-1 to the ionization limit, using the technique of resonant two-photon ionization spectroscopy with mass spectrometric detection. Adiabatic ionization potentials have been accurately measured for both molecules,...

  • On the determination of cluster properties by ionization techniques. Booze, Jon A.; Baer, Tomas // Journal of Chemical Physics;4/1/1992, Vol. 96 Issue 7, p5541 

    Experimental differentiation between similar cluster ions of Acetylene produced by the chemical reactions is reported. (AIP)

  • The heats of formation of some C2H5O+ isomers, relevant bond energies in ethanol and PA(CH3CHO). Ruscic, B.; Berkowitz, J. // Journal of Chemical Physics;12/15/1994, Vol. 101 Issue 12, p10936 

    The ionization potentials of the transient species CH3CH2O, CH3CHOH, and CH2CH2OH (generated by the F+ethanol reactions) are measured by photoionization mass spectrometry: I.P.(CD3CD2O)=10.29±0.08 eV (tentative), I.P.(CH3CHOH)<6.85 eV, and I.P.(CD2CH2OH) ≤8.35±0.06 eV. The latter...

  • Stabilities and structures of gas phase MgO clusters. Ziemann, Paul J.; Castleman, A. W. // Journal of Chemical Physics;1/1/1991, Vol. 94 Issue 1, p718 

    Gas phase (MgO)+n and (MgO)nMg+ clusters (n≤90) were produced in a gas aggregation source and studied by using laser-ionization time-of-flight mass spectrometry. The abundance maxima observed in the mass spectra indicate that the clusters form compact cubic structures similar to pieces of...

  • Photoionization spectra and ionization thresholds of CH3SO, CH3SOH, and CH3SS(O)CH3. Hung, Wen-Ching; Shen, Min-yi; Lee, Yuan-Pern; Wang, Niann-Shiah; Cheng, Bing-Ming // Journal of Chemical Physics;11/1/1996, Vol. 105 Issue 17, p7402 

    Photoionization efficiency (PIE) spectra of CH3SO, CH3SOH, and CH3SS(O)CH3 were measured in the spectral range 110–150 nm by means of a discharge flow system coupled to a photoionization mass spectrometer that employs a synchrotron radiation source. These species were generated by...

  • Direct determination of the adiabatic ionization energy of NO2 as measured by guided ion-beam mass spectrometry. Clemmer, D. E.; Armentrout, P. B. // Journal of Chemical Physics;8/15/1992, Vol. 97 Issue 4, p2451 

    The adiabatic ionization energy (IE) of NO2 is measured to be 9.60±0.03 eV by studying the charge-transfer reactions of Zn+, NO+, and CH3I+ with NO2 and those of NO+2 with α,α,α-trifluorotoluene and CH3I using guided ion-beam mass spectrometry. This value confirms the accuracy of a...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics