TITLE

A 2D NMR method to study peptide phosphorylation

AUTHOR(S)
Raeck, Christian; Berger, Stefan
PUB. DATE
December 2007
SOURCE
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2161
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We demonstrate a 2D NMR method which distinguishes between phosphorylated and non-phosphorylated amino acids. The method is capable of monitoring the amino acid and site-specific enzymatic phosphorylation and dephosphorylation of peptides. The method was developed using O-phosphorylated amino acids and its potential is shown with a peptide fragment of the myelin basic protein (MBP).
ACCESSION #
27657805

 

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