A 2D NMR method to study peptide phosphorylation

Raeck, Christian; Berger, Stefan
December 2007
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2161
Academic Journal
We demonstrate a 2D NMR method which distinguishes between phosphorylated and non-phosphorylated amino acids. The method is capable of monitoring the amino acid and site-specific enzymatic phosphorylation and dephosphorylation of peptides. The method was developed using O-phosphorylated amino acids and its potential is shown with a peptide fragment of the myelin basic protein (MBP).


Related Articles

  • A 'three-pronged' binding mechanism for the SAP/SH2D1A SH2 domain: structural basis and relevance to the XLP syndrome. Hwang, Peter M.; Chengjun Li; Morra, Massimo; Lillywhite, Jennifer; Muhandiram, D. Ranjith; Gertler, Frank; Terhorst, Cox; Kay, Lewis E.; Pawson, Tony; Forman-Kay, Julie D.; Shun-Cheng Li // EMBO Journal;2/1/2002, Vol. 21 Issue 3, p314 

    The SH2 domain protein SAP/SH2D1A, encoded by the X-linked lymphoproliferative (XLP) syndrome gene, associates with the hematopoietic cell surface receptor SLAM in a phosphorylation-independent manner. By screening a repertoire of synthetic peptides, the specificity of SAP/SH2D1A has been mapped...

  • Peptide binding proclivities of calcium loaded calbindin-D28k Kordys, David R.; Bobay, Benjamin G.; Thompson, Richele J.; Venters, Ronald A.; Cavanagh, John // FEBS Letters;Oct2007, Vol. 581 Issue 24, p4778 

    Abstract: Calbindin-D28k is known to function as a calcium-buffering protein in the cell. Moreover, recent evidence shows that it also plays a role as a sensor. Using circular dichroism and NMR, we show that calbindin-D28k undergoes significant conformational changes upon binding calcium,...

  • Solution state structures of human pancreatic amylin and pramlintide. Cort, John R.; Zhihong Liu; Lee, Gregory M.; Huggins, K.N.L.; Janes, Susan; Prickett, Kathryn; Andersen, Niels H. // PEDS: Protein Engineering, Design & Selection;Aug2009, Vol. 22 Issue 8, p497 

    We have employed pramlintide (prAM) as a surrogate for hAM in CD and NMR studies of the conformational preferences of the N-terminal portion of the structure in media which do not provide long-lived monomeric solutions of hAM due to its rapid conversion to preamyloid β aggregate states....

  • Determination of methyl 13C–15N dipolar couplings in peptides and proteins by three-dimensional and four-dimensional magic-angle spinning solid-state NMR spectroscopy. Helmus, Jonathan J.; Nadaud, Philippe S.; Höfer, Nicole; Jaroniec, Christopher P. // Journal of Chemical Physics;2/7/2008, Vol. 128 Issue 5, p052314 

    We describe three- and four-dimensional semiconstant-time transferred echo double resonance (SCT-TEDOR) magic-angle spinning solid-state nuclear magnetic resonance (NMR) experiments for the simultaneous measurement of multiple long-range 15N–13Cmethyl dipolar couplings in uniformly 13C,...

  • Investigating the inclusion properties of aromatic amino acids complexing beta-cyclodextrins in model peptides. Caso, Jolanda; Russo, Luigi; Palmieri, Maddalena; Malgieri, Gaetano; Galdiero, Stefania; Falanga, Annarita; Isernia, Carla; Iacovino, Rosa // Amino Acids;Oct2015, Vol. 47 Issue 10, p2215 

    Cyclodextrins are commonly used as complexing agents in biological, pharmaceutical, and industrial applications since they have an effect on protein thermal and proteolytic stability, refolding yields, solubility, and taste masking. β-cyclodextrins (β-CD), because of their cavity size are...

  • Structure of the negative regulatory domain of p53 bound to S100B(ββ). Rust, Richard R.; Baldisseri, Donna M.; Weber, David J. // Nature Structural Biology;Jul2000, Vol. 7 Issue 7, p570 

    A Ca2+ dependent conformational change in dimeric S100B(ββ) is required for it to bind p53 and inhibit phosphorylation of this tumor suppressor in its C-terminal negative regulatory domain. A peptide derived from this region of p53 (residues 367?388) was found to have no regular structure...

  • Synthesis and Structure of Bis-phosphorylated 1,4:3,6-Dianhydro-D-mannitol Derivatives. Grachev, M. K.; Soboleva, N. O.; Kurochkina, G. I.; Vasyanina, L. K.; Bel'skii, V. K.; Nifant'ev, E. E. // Russian Journal of General Chemistry;Jun2003, Vol. 73 Issue 6, p903 

    A number of bis-phosphorylated 1,4:3,6-dianhydro-D-mannitol derivatives were synthesized. The increased steric loading of the phosphorus-containing residues in the compounds prepared gives rise to complicated 1H and 31P NMR spectra and magnetic nonequivalence of structurally similar molecular...

  • 31P NMR study on the autophosphorylation of insulin receptors in the plasma membrane. Yili Ge; Hong Peng; Kaixun Huang // Analytical & Bioanalytical Chemistry;Aug2006, Vol. 385 Issue 5, p834 

    A nonradioactive 31P nuclear magnetic resonance (NMR) spectroscopy protocol has been developed and used to investigate in vitro autophosphorylation of insulin receptors. Optimum experimental conditions have been explored, and the effects of Mn2+ and phosphocreatine (PCr) on the determination of...

  • Tris-phosphorylated esters of symmetrical and unsymmetrical triols. Pugashova, N. M.; Koroteev, A. M.; Bushmarinov, I. S.; Lysenko, K. A.; Andriutse, E. N.; Koroteev, M. P.; Nifant’ev, E. E. // Russian Journal of General Chemistry;May2008, Vol. 78 Issue 5, p883 

    By reaction of a series of triols and monosaccharides with 5,5-dimethyl-2-chloro-1,3,2-dioxaphosphorinane their tris-phosphorylated derivatives were synthesized, and the simplest chemical transformations of the latter were studied. Structures of the obtained P(V) derivatives were confirmed by...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics