Ex-vivo NMR of unprocessed tissue in water: a simplified procedure for studying intracranial neoplasms

Maosheng Xu; Jieru Ye; Daiwen Yang; Xiaoli Xu; Tseng Tsai Yeo; Wai Hoe Ng; C. C. Lim
December 2007
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2153
Academic Journal
Ex-vivo and in-vitro nuclear magnetic resonance (NMR) spectroscopy techniques have been used for studying chemical metabolites in surgically resected specimens of human neoplasms, and may provide complementary information to in-vivo whole-body magnetic-resonance spectroscopy (MRS). We describe an ex-vivo NMR in water method for measurement of water-soluble metabolites in unprocessed normal rat brain tissue and human intracranial neoplasms. The NMR spectra obtained using the method described here were comparable to those obtained using high-resolution magic-angle spinning (HRMAS) NMR methods, with good correlation in metabolite concentrations relative to creatine ( r 2 = 0.7635). Improved spectral resolution and baseline were noted compared to HRMAS, but macromolecule resonances were not detected. Ex-vivo NMR of unprocessed tissue in water is rapid and technically simple to perform, and has the potential to be used for direct assessment of intracranial neoplasms.


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