TITLE

Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol

AUTHOR(S)
Kolosova, Anna Yu; De Saeger, Sarah; Sibanda, Liberty; Verheijen, Ron; Van Peteghem, Carlos
PUB. DATE
December 2007
SOURCE
Analytical & Bioanalytical Chemistry;Dec2007, Vol. 389 Issue 7/8, p2103
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually. A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 μg kg−1 for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective on-site screening technique for the simultaneous determination of mycotoxins in grain samples.
ACCESSION #
27657793

 

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