TITLE

Smad7 gene transfer inhibits peritoneal fibrosis

AUTHOR(S)
Nie, J.; Dou, X.; Hao, W.; Wang, X.; Peng, W.; Jia, Z.; Chen, W.; Li, X.; Luo, N.; Lan, H. Y.; Yu, X. Q.
PUB. DATE
December 2007
SOURCE
Kidney International;Dec2007, Vol. 72 Issue 11, p1336
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Fibrosis mediated by transforming growth factor-β (TGF-β) is a common cause of peritoneal dialysis (PD) failure. In a model of peritoneal fibrosis, we tested the effect of Smad7, an inhibitor of TGF-β signaling, using an ultrasound-microbubble-mediated delivery system. Rats were given daily PD for 4 weeks and received Smad7 or control plasmid transfer. The ultrasound technique enhanced Smad7 expression in a dose-dependent manner in more than 80% of the peritoneal cells after 3 days. The expression decreased by 14 days, but this was corrected by a second gene transfer. The overexpression of Smad7 substantially inhibited Smad2/3 activation, TGF-β, plasminogen activator inhibitor-1, extracellular matrix, and myofibroblast mRNA, and protein expression in the peritoneal cells. The decreased peritoneal injury included the rise of mass transfer of glucose, a reduction of the ultrafiltration rate, and fibrotic thickening. Our studies suggest that ultrasound-mediated Smad7 gene delivery may be useful in the prevention or treatment of dialysis-induced peritoneal fibrosis.Kidney International (2007) 72, 1336–1344; doi:10.1038/sj.ki.5002533; published online 12 September 2007
ACCESSION #
27488709

 

Related Articles

  • Connective tissue growth factor and its regulation in the peritoneal cavity of peritoneal dialysis patients. Zarrinkalam, Krystyna H.; Stanley, Jodie M.; Gray, Julia; Oliver, Noelynn; Faull, Randall J. // Kidney International;Jul2003, Vol. 64 Issue 1, p331 

    Connective tissue growth factor and its regulation in the peritoneal cavity of peritoneal dialysis patients . Background. Connective tissue growth factor (CTGF) is a fibrogenic cytokine that is highly expressed in wound healing and fibrotic lesions. The role of transforming growth factor-β...

  • HB-EGF is produced in the peritoneal cavity and enhances mesothelial cell adhesion and migration. Faull, Randall J.; Stanley, Jodie M.; Fraser, Scott; Power, David A.; Leavesley, David I. // Kidney International;Feb2001, Vol. 59 Issue 2, p614 

    Focuses on the mechanism of Heparin-binding epidermal growth factor-like growth factor (HB-EGF) in continuous ambulatory peritoneal dialysis (CAPD) patients. Role of HB-EGF in wound repair and fibrogenic in the peritoneal cavity; Immunochemistry of the flow cytometry of HB-EGF effects;...

  • Human peritoneal fibroblast proliferation in 3-dimensional culture: Modulation by cytokines, growth factors and peritoneal dialysis effluent. Beavis, M. Janine; Williams, John D.; Hoppe, Jürgen; Topley, Nicholas // Kidney International;Jan1997, Vol. 51 Issue 1, p205 

    The article studies the human peritoneal fibroblast (HPFB) and examines its proliferate responses to cytokines and growth factors as well as dialysis effluent obtained from patients during peritoneal infection. The study was done by establishing a 3-dimensional cell culture system. Experiments...

  • Management of Scleroderma-related End-stage Renal Disease With Automated Peritoneal Dialysis. Pavan, Malleshappa; Aiyangar, Ashwinikumar; Chaudhari, Anup; Rajputh, Prashanth; Ranganath, Ravi; Babulal, Shah Arunkumar // Iranian Journal of Kidney Diseases;Apr2010, Vol. 4 Issue 2, p162 

    Kidney failure is the principal cause of death in scleroderma and accounts for at least 50% of deaths in this disease. Management of scleroderma-related end-stage renal disease requires some form of renal replacement therapy. Survival up to 18 months has been reported in one patient on...

  • A microrna screen to identify regulators of peritoneal fibrosis in a rat model of peritoneal dialysis. Fan Lin; Xu Wu; Huidi Zhang; Xiaohan You; Zhoucang Zhang; Rongrong Shao; Chaoxing Huang // BMC Nephrology;Aug2015, Vol. 16 Issue 1, p1 

    Background: Peritoneal fibrosis is a common complication in patients treated with long-term peritoneal dialysis. The aim of this study was to identify the microRNAs (miRNAs) involved in regulation of peritoneal fibrosis in a rat model of peritoneal dialysis. Methods: Twenty-four Sprague-Dawley...

  • REFRESHER QUIZ.  // Clinical Pediatrics;Dec1966, Vol. 5 Issue 12, p759 

    Presents a quiz on peritoneal dialysis, cystic fibrosis, and heart failure.

  • CAPD: A dialysis breakthrough with its own burdens. Malti, Jossette; Wellons, Debra // RN;Jan88, Vol. 51 Issue 1, p46 

    Reports on continuous ambulatory peritoneal dialysis (CAPD). Drawbacks of hemodialysis; Advantages of CAPD; Guidelines; Steps. INSETS: A step-by-step guide to CAPD;A troubleshooting guide for CAPD.

  • Higher Transport Status Associated with Increased Risk of Death.  // Kidney;Jan/Feb99, Vol. 8 Issue 1, p25 

    Presents the findings of a study which evaluated the association of peritoneal membrane transport with technique and patient survival. Assessment of the adequacy of continuous ambulatory peritoneal dialysis Association of higher peritoneal transport with increased risk of either technique...

  • Peritoneal Dialysis and Hemodialysis of Tritiated Digoxin. Ackerman, George L.; Doherty, James E.; Flanigan, William J. // Annals of Internal Medicine;Oct67, Vol. 67 Issue 4, p718 

    Presents information on a study which analyzed the metabolism of digoxin in patients undergoing hemodialysis and peritoneal dialysis. Results of in vitro hemodialysis performed; Peritoneal clearance rate of tritiated digoxin; Results of the experiment comparing the dialysis of digoxin from...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics