TITLE

A method for identification of diastereomers of 2-decarboxy-betacyanins and 2,17-bidecarboxy-betacyanins in reversed-phase HPLC

AUTHOR(S)
Wybraniec, Slawomir
PUB. DATE
November 2007
SOURCE
Analytical & Bioanalytical Chemistry;Nov2007, Vol. 389 Issue 5, p1611
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A method was developed for identification of diastereomers of 2-decarboxy-betacyanins and 2,17-bidecarboxy-betacyanins chromatographed in reversed-phase high-performance liquid chromatography (HPLC) as pairs of unknown elution order. The method was based on alkaline hydrolysis of selected betacyanin and decarboxylated betacyanin mixtures and subsequent cross-recondensation of the hydrolysates. The arising intermediate derivatives of decarboxylated betalamic acid and cyclo-dopa were stable enough for subsequent recondensation. Generated diagnostic pigments as the recondensation products were monitored by HPLC–diode-array detection–electrospray ionisation mass spectrometry. The isoforms (15 R) of 2-decarboxy-betacyanins and 2,17-bidecarboxy-betacyanins were eluted earlier than the 15 S forms, in contrast to betacyanins and 17-decarboxy-betacyanins, but in accordance with betaxanthins. 2,17-Bidecarboxy-betanin/2,17-bidecarboxy-isobetanin, being not resolved in reversed-phase HPLC, were partially separated by ion-pair chromatography and under these conditions their order of elution was the same as that of acylated 2,17-bidecarboxy-betacyanins. The method allows complete identification of all the decarboxylated groups of betanin, phyllocactin and hylocerenin as well as other betacyanins in biological or pharmaceutical material.
ACCESSION #
27251459

 

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