TITLE

Validación de un ELISA para la cuantificación de IgG anti- antipolisacárido capsular de polisacárido Neisseria meningitidis serogrupo C en sueros de ratones

AUTHOR(S)
Cuello, Maribel; Acosta, Yanely; Martínez, Juan C.; Cabrera, Osmir; Ochoa, Rolando; Balboa, Julio A.; Soto, Carmen R.; Martínez, Miguel E.; Sierra, Gustavo
PUB. DATE
October 2001
SOURCE
Revista VacciMonitor (Vacunología y Temas Afines);Oct2001, Vol. 10 Issue 4, p7
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An indirect enzyme-linked immunoabsorbent assay (ELISA) was developed to determine antibody anti-polysaccharide C mouse IgG. Polystyrene plates precoating with poly-L-lysine, then Neisseria meningitidis group C polysaccharide (Finlay Institute, Havana, Cuba) was used to evaluated the immune response against this component in a candidate vaccine in pre-clinical studies. Alkaline phosphatase conjugate was used for recognizing mouse antibodies against polysaccharide C. The enzymatic reaction is evidenced by using the specific substrate p-nitrophenylphosphate. The detection limit of the assay was 123,74 U/mL, the specificity of assay was high. Intra-and interassay lack of precision, parallelism, linearity, and recovery were ± 10% of the expected values.
ACCESSION #
27104809

 

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