Quantitative Proteomics: Available Tools and Results of a Collaborative Study

Wehr, Tim
October 2007
LC-GC North America;Oct2007, Vol. 25 Issue 10, p1030
Trade Publication
The article discusses methods that are used for quantitative proteomics. Gel electrophoresis was the primary technique for protein analysis in complex mixtures and is a quantitative tool used by laboratories. The stable isotope method labeled the cell proteome by one or more amino acids and dealt on cells that can be grown in culture and blend exogenous amino acid into protein. Label-free methods are an option to metabolic labeling and introduction of stable isotopes by chemical derivatization.


Related Articles

  • Chemical proteomics: a powerful tool for exploring protein lipidation. Storck, Elisabeth M.; Serwa, Remigiusz A.; Tate, Edward W. // Biochemical Society Transactions;Feb2013, Vol. 41 Issue 1, p56 

    The study of post-translational modifications such as protein lipidation is a non-trivial challenge of the postgenomic era. In recent years the field of chemical proteomics has greatly advanced our ability to identify and quantify protein lipidation. In the present review, we give a brief...

  • Pinnacle: a fast, automatic and accurate method for detecting and quantifying protein spots in 2-dimensional gel electrophoresis data. Jeffrey S. Morris; Brittan N. Clark; Howard B. Gutstein // Bioinformatics;Feb2008, Vol. 24 Issue 4, p529 

    Motivation: One of the key limitations for proteomic studies using 2-dimensional gel electrophoresis (2DE) is the lack of rapid, robust and reproducible methods for detecting, matching and quantifying protein spots. The most commonly used approaches involve first detecting spots and drawing spot...

  • miRNAs: small changes, widespread effects. Linsen, Sam EV; Tops, Bastiaan BJ; Cuppen, Edwin // Cell Research;Dec2008, Vol. 18 Issue 12, p1157 

    The article discusses papers that address the identification and verification of large-scale microRNA targets. This was accomplished by combining stable isotope labeling with amino acids in ell culture (SILAC) and state-of-the-art mass spectroscopy. Data analysis revealed numerous features of...

  • Large Gel Two-Dimensional Electrophoresis: Improving Recovery of Cellular Proteome. Inagaki, Naoyuki; Katsuta, Kazuhiro // Current Proteomics;Jan2004, Vol. 1 Issue 1, p35 

    One of the goals of expression proteomics is to display and analyze all the proteins in a particular proteome. Cells are thought to comprise tens of thousands of proteins expressed in a dynamic range of 1-10 5 or 10 6. Low recovery of cellular proteome leads to a gross loss of important...

  • Web Based Theoretical Protein pI, MW and 2DE Map. Brum, Itaraju J. B.; Martins-de-Souza, Daniel; Smolka, Marcus B.; Novello, José C.; Galembeck, Eduardo // Journal of Computer Science & Systems Biology;Jan-Feb2009, Vol. 2 Issue 1, p93 

    The genomic projects have provided a far wide amount of information that still requires be analyzing and interpreting. That would be impossible to be done without the development of well adapted computational tools that might help the analysis of these data we have collected so far. Due to the...

  • Novel 'omics' approach for study of low-abundance, low-molecular-weight components of a complex biological tissue: regional differences between chorionic and basal plates of the human placenta. Kedia, Komal; Nichols, Caitlin; Thulin, Craig; Graves, Steven // Analytical & Bioanalytical Chemistry;Nov2015, Vol. 407 Issue 28, p8543 

    Tissue proteomics has relied heavily on two-dimensional gel electrophoresis, for protein separation and quantification, then single protein isolation, trypsin digestion, and mass spectrometric protein identification. Such methods are predominantly used for study of high-abundance, full-length...

  • Out with the old? Schaffer, Catherine // Drug Discovery & Development;Dec2007, Vol. 10 Issue 12, p44 

    The article discusses ways to improve the two-dimensional polyacrylamide gel electrophoresis (2D PAGE) technology. The tool is considered as a reliable technique with proven track record for identification and qualitative analysis of proteins. It covers a complex protein sample resolution and...

  • Protein-based stable isotope probing (Protein-SIP) reveals active species within anoxic mixed cultures. Jehmlich, Nico; Schmidt, Frank; von Bergen, Martin; Richnow, Hans-Hermann; Vogt, Carsten // ISME Journal: Multidisciplinary Journal of Microbial Ecology;Nov2008, Vol. 2 Issue 11, p1122 

    It is still a challenge to link specific metabolic activities to certain species in a microbial community because of methodological limitations. We developed a method to analyze the specific metabolic activity of a single bacterial species within a consortium making use of [13C7]-toluene for...

  • Identifying trophic variation in a marine suspension feeder: DNA- and stable isotope-based dietary analysis in Mytilus spp. Maloy, Aaron; Nelle, Peter; Culloty, Sarah; Slater, John; Harrod, Chris // Marine Biology;Feb2013, Vol. 160 Issue 2, p479 

    Accurate field data on trophic interactions for suspension feeders are lacking, and new approaches to dietary analysis are necessary. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was integrated with stable isotope analysis to examine dietary patterns in...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics