TITLE

A Role for TGF-β1-Induced Cellular Responses during Wound Healing of the Non-Scarring Early Human Fetus?

AUTHOR(S)
Rolfe, Kerstin J.; Richardson, Janette; Vigor, Charlotte; Irvine, Laurie M.; Grobbelaar, Addie O.; Linge, Claire
PUB. DATE
November 2007
SOURCE
Journal of Investigative Dermatology;Nov2007, Vol. 127 Issue 11, p2656
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Early human fetuses regenerate cutaneous wounds perfectly without scarring. However, transforming growth factor-β1 (TGF-β1), the cytokine linked with scarring in mature tissue, is also present during fetal wound repair, albeit transiently. We present a comparison of response to TGF-β1 by fibroblasts derived from early human fetal skin (non-scarring) and their mature (scarring) postnatal counterparts, which revealed that although fetal fibroblasts do indeed differentiate into myofibroblasts, this response is altogether more rapid and short-lived. Fetal fibroblasts also failed to exhibit the TGF-β1-induced increase in collagen (mRNA and protein) demonstrated by their postnatal counterparts. Fetal cells exhibited a comparatively short-lived or rapid phosphorylation of several components of the TGF-β1 signaling pathways: Smad2/3 and c-Jun N-terminal kinase. Unlike quiescent postnatal fibroblasts, quiescent fetal fibroblasts also phosphorylated extracellular signal-regulated kinases in response to TGF-β1. These altered responses to TGF-β1 may well contribute to the transition between perfect regeneration and scar formation seen during development.Journal of Investigative Dermatology (2007) 127, 2656–2667; doi:10.1038/sj.jid.5700951; published online 9 August 2007
ACCESSION #
27042439

 

Related Articles

  • Transforming Growth Factor Beta 3 Is Required for Excisional Wound Repair In Vivo. Le, Mark; Naridze, Rachelle; Morrison, Jasmine; Biggs, Leah C.; Rhea, Lindsey; Schutte, Brian C.; Kaartinen, Vesa; Dunnwald, Martine // PLoS ONE;Oct2012, Vol. 7 Issue 10, Special section p1 

    Wound healing is a complex process that relies on proper levels of cytokines and growth factors to successfully repair the tissue. Of particular interest are the members of the transforming growth factor family. There are three TGF-β isoforms--TGF-β 1, 2, and 3, each isoform showing a...

  • Human Granulation-tissue Fibroblasts Show Enhanced Proteoglycan Gene Expression and Altered Response to TGF-β1. Häkkinen, L.; Westermarck, J.; Kähäri, V.-M.; Larjava, H. // Journal of Dental Research;Oct1996, Vol. 75 Issue 10, p1767 

    Granulation-tissue fibroblasts are phenotypically unique cells that play an important role in wound repair and the development of chronic inflammatory lesions in connective tissue. In the present study, we compared proteoglycan, type I, and type III procollagen gene expression by...

  • Alpha-melanocyte stimulating hormone suppresses the proliferation of human Tenon's capsule fibroblast proliferation induced by transforming growth factor beta 1. Zhang, Zheng; Ma, Jin; Yao, Ke; Yin, Jinfu // Molecular Biology;Jul2012, Vol. 46 Issue 4, p563 

    Alpha-melanocyte stimulating hormone (α-MSH) is a proopiomelanocortin derivative and a multi-function neuropeptide, well know for its pigment-inducing capacity, inhibitory action on proinflammatory cytokines and chemoattractant cytokines, and suppressive action on collagen synthesis. Human...

  • TRANSFORMING GROWTH FACTOR-BETA3 (TGF-β3) EXPRESSES ANTI-FIBROTIC PROPERTIES IN PANCREATIC STELLATE CELLS. Shek, F. W.; Li, K. F.; Mann, J.; Williams, E. J.; Iredale, J. P.; Beynon, R. C.; Fine, D. R. // Gut;Apr2004 Supplement 3, Vol. 53, pA16 

    Pancreatic stellate cells (PSC) play an important role in the production of extracellular matrix in pancreatic fibrosis. In contrast to TGF-β1, TGF-β3 appears to have anti-scanning properties in cutaneous wound healing. The study examined the role of TGF-β3 in the deposition of collagen...

  • Effects of TGFβ1 on Dental Pulp Cells in Cultured Human Tooth Slices. Melin, M.; Joffre-Romeas, A.; Farges, J.-C.; Couble, M.-H.; Magloire, H.; Bleicher, F. // Journal of Dental Research;Sep2000, Vol. 79 Issue 9, p1689 

    Transforming Growth Factor-beta1 (TGFβ1) is a potent modulator of tissue repair in various tissues. To analyze its role during human dental repair, we used thick-sliced teeth cultured as described previously (Magloire et al., 1996). The supply of TGFβ1 to the pulp tissue was accomplished...

  • ALK5 Inhibition Blocks TGFβ-induced CCN2 Expression in Gingival Fibroblasts. Thompson, K.; Hamilton, D.W.; Leask, A. // Journal of Dental Research;12/01/2010, Vol. 89 Issue 12, p1450 

    Connective tissue growth factor (CCN2/CTGF) is not normally expressed in gingival fibroblasts, but is induced by the potent profibrotic cytokine TGFβ and is overexpressed in gingival fibrosis. Since CCN2 is a marker and mediator of fibrosis, targeting CCN2 expression in gingival fibroblasts...

  • Quantitative Proteomics of Inhibited TGF-Beta-Signaling Networks in Human Keloid Fibroblasts. Flynn, Charles R.; Zhengping Yi; Lopes, Luciana; Mandarino, Lawrence; Brophy, Colleen M.; Meyer, Christian // Diabetes;Jun2007 Supplement 1, Vol. 56, pA509 

    Tissue fibrosis, oxidative stress, inflammation and mitochondrial dysfunction are pathological features of diabetic nephropathy, neuropathy, retinopathy as well as abnormalities involving the skeletal muscle and liver. One trigger of these responses is the fibrogenic cytokine, transforming...

  • TNF-α Suppresses α-Smooth Muscle Actin Expression in Human Dermal Fibroblasts: An Implication for Abnormal Wound Healing. Goldberg, Mytien T.; Yuan-Ping Han; Chunli Yan; Shaw, Michael C.; Garner, Warren L. // Journal of Investigative Dermatology;Nov2007, Vol. 127 Issue 11, p2645 

    Abnormal wound healing encompasses a wide spectrum, from chronic wounds to hypertrophic scars. Both conditions are associated with an abnormal cytokine profile in the wound bed. In this study, we sought to understand the dynamic relationships between myofibroblast differentiation and mechanical...

  • Connective Tissue Growth Factor in Indomethacin-Induced Rat Gastric Ulcer. Lempinen, M.; lnkinen, K.; Wolff, H.; Ahonen, J. // European Surgical Research;2002, Vol. 34 Issue 3, p232 

    The healing of gastric ulcers requires not only the complete epithelial covering but also the restitution of connective tissue. Transforming growth factor-β (TGF-β) and its downstream mediator, connective tissue growth factor (CTGF), are potent stimulators for connective tissue formation...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics