TITLE

Efficient Elimination of Multidrug-Resistant Staphylococcus aureus by Cloned Lysin Derived from Bacteriophage øMR11

AUTHOR(S)
Rashel, Mohammad; Uchiyama, Jumpei; Ujihara, Takako; Uehara, Yoshio; Kuramoto, Shu; Sugihara, Shigeyoshi; Yagyu, Ken-Ichi; Muraoka, Asako; Sugai, Motoyuki; Hiramatsu, Keiichi; Honke, Koichi; Matsuzaki, Shigenobu
PUB. DATE
October 2007
SOURCE
Journal of Infectious Diseases;10/15/2007, Vol. 196 Issue 8, p1237
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report the successful purification of a cloned lysin encoded by the novel Staphylococcus aureus bacteriophage φMR11. The lysin, designated MV-L, rapidly and completely lysed cells of a number of S. aureus strains tested, including methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus and a subset of vancomycin- intermediate S. aureus (VISA) in growing conditions. MV-L-mediated killing is specific to S. aureus and not to other species, except for S. simulans. MV-L exerted its staphylocidal effect synergistically with glycopeptide antibiotics against VISA. MV-L efficiently eliminated MRSA that had been artificially inoculated into the nares of mice. The intraperitoneal administration of MV-L also protected mice against MRSA septic death, without any harmful effects. Although MV-L evoked detectable levels of a humoral response in mice, the antibodies did not abolish the bacteriolytic activity. These results indicate that MV-L might be useful as a powerful therapeutic agent against multidrug-resistant S. aureus infections.
ACCESSION #
26923564

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics