Inadequate Blood Volume Collected for Culture: A Survey of Health Care Professionals

Donnino, Michael W.; Goyal, Nikhil; Terlecki, Theresa M.; Donnino, Kathryn F.; Miller, Joseph B.; Otero, Ronny M.; Howell, Michael D.
September 2007
Mayo Clinic Proceedings;Sep2007, Vol. 82 Issue 9, p1069
Academic Journal
Adequate blood culture volume is the single most important determinant for yield of organisms. We sought to determine whether health care professionals are aware of the current evidence-based recommendations for blood culture collection. An anonymous survey of employees qualified to collect blood cultures was conducted (July-October 2004) in an urban tertiary care facility. The survey asked, ‘What volume of blood should be collected in one bottle for a blood culture?’ Of the 360 employees of the hospital surveyed, 355 returned evaluable answers for blood culture volume collected. Overall, 79% (95% confidence interval [CI], 74%-83%) answered less than 10 mL, and 44% (95% CI, 39%-49%) answered less than 5 mL of blood. When examined by occupation, 90% (95% CI, 86%-94%) of nurses, 97% (95% CI, 91%-100%) of technicians, and 55% (95% CI, 46%-64%) of physicians answered less than 10 mL; 52% (95% CI, 45%-59%) of nurses, 63% (95% CI, 46%-79%) of technicians, and 26% (95% CI, 18%-35%) of physicians answered less than 5 mL. Of all respondents, 21% (95% CI, 17%-25%) answered 1 mL or less. Our findings reveal that a high percentage of health care personnel do not know the optimal volume of blood recommended for collection. Because volume remains the most important determinant for the optimal yield of organisms, these findings raise an important quality assurance issue.


Related Articles

  • Host and microbe determinants that may influence the success of S. aureus colonization. Johannessen, Mona; Sollid, Johanna E.; Hanssen, Anne-Merethe // Frontiers in Cellular & Infection Microbiology;May2012, Vol. 2, p1 

    Staphylococcus aureus may cause serious skin and soft tissue infections, deep abscesses, endocarditis, osteomyelitis, pneumonia, and sepsis. S. aureus persistently colonizes 25-30% of the adult human population, and S. aureus carriers have an increased risk for infections caused by the...

  • SVM-based prediction of linear B-cell epitopes using Bayes Feature Extraction. Wee, Lawrence J. K.; Simarmata, Diane; Yiu-Wing Kam; Ng, Lisa F. P.; Joo Chuan Tong // BMC Genomics;Jan2010 Supplement 4, Vol. 11, p1 

    Backgound: The identification of B-cell epitopes on antigens has been a subject of intense research as the knowledge of these markers has great implications for the development of peptide-based diagnostics, therapeutics and vaccines. As experimental approaches are often laborious and time...

  • GPI-anchored single chain Fv - an effective way to capture transiently-exposed neutralization epitopes on HIV-1 envelope spike. Michael Wen; Arora, Reetakshi; Huiqiang Wang; Lihong Liu; Kimata, Jason T.; Paul Zhou // Retrovirology;2010, Vol. 7, p79 

    Background: Identification of broad neutralization epitopes in HIV-1 envelope spikes is paramount for HIV-1 vaccine development. A few broad neutralization epitopes identified so far are present on the surface of native HIV-1 envelope spikes whose recognition by antibodies does not depend on...

  • Insertion of a myc-tag within α-dystroglycan domains improves its biochemical and microscopic detection. Morlacchi, Simona; Sciandra, Francesca; Bigotti, Maria Giulia Bigotti; Bozzi, Manuela; H�bner, Wolfgang; Galtieri, Antonio; Giardina, Bruno; Brancaccio, Andrea // BMC Biochemistry;2012, Vol. 13 Issue 1, p14 

    Background: Epitope tags and fluorescent fusion proteins have become indispensable molecular tools for studies in the fields of biochemistry and cell biology. The knowledge collected on the subdomain organization of the two subunits of the adhesion complex dystroglycan (DG) enabled us to insert...

  • Immunoabsorbent. Vohr, Hans-Werner // Encyclopedic Reference of Immunotoxicology;2005, p325 

    A definition of the term "immunoabsorbent" is presented. The term refers to an antigen or antigenic determinant bound to inert cartier material such as agarose, sepharose, or latex. It may also be used to specifically absorb and isolate antibodies or cells of defined specificity from...

  • Xenogeneic. Vohr, Hans Werner // Encyclopedic Reference of Immunotoxicology;2005, p703 

    A definition of the term "xenogeneic" is presented. This refers to the genetic relationship between individuals of different species, with respect to antigenicity.

  • Characterization of the peptide-binding specificity of Mamu-A*11 results in the identification of SIV-derived epitopes and interspecies cross-reactivity. Sette, Alessandro; Sidney, John; Bui, Huynh-Hoa; Guercio, Marie-France; Alexander, Jeff; Loffredo, John; Watkins, David; Mothé, Bianca // Immunogenetics;Apr2005, Vol. 57 Issue 1/2, p53 

    The SIV-infected Indian rhesus macaque is the most established model of HIV infection, providing insight into pathogenesis and a system for testing novel vaccines. However, only a limited amount of information is available regarding the peptide-binding motifs and epitopes bound by their class I...

  • Central Role of the Holliday Junction Helicase RuvAB in visE Recombination and Infectivity of Borrella burgdorferi. Tao Lin; Lihui Gao; Edmondson, Diane G.; Jacobs, Mary B.; Philipp, Mario T.; Norris, Steven J. // PLoS Pathogens;Dec2009, Vol. 5 Issue 12, p1 

    Antigenic variation plays a vital role in the pathogenesis of many infectious bacteria and protozoa including Borrelia burgdorferi, the causative agent of Lyme disease. VlsE, a 35 kDa surface-exposed lipoprotein, undergoes antigenic variation during B. burgdorferi infection of mammalian hosts,...

  • Genetic and antigenic analysis of chlamydial isolates from cats in the Emilia­Romagna region of northern Italy. di Francesco, A.; Donati, M.; Cavrini, F.; Piva, S.; Cevenini, R.; Baldelli, R. // Veterinary Record: Journal of the British Veterinary Association;9/10/2005, Vol. 157 Issue 11, p325 

    Reports on the genetic and antigenic analysis of chlamydial isolates from cats in the Emilia-Romagna, Italy. Evaluation of the prevalence of feline chlamydiosis; Initiation of surveillance program of the health status of colonies of cats in the region; Extraction of DNA from the cultures using a...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics