TITLE

Detection of avian influenza virus using an interferometric biosensor

AUTHOR(S)
Jie Xu; Suarez, David; Gottfried, David S.
PUB. DATE
October 2007
SOURCE
Analytical & Bioanalytical Chemistry;Oct2007, Vol. 389 Issue 4, p1193
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An interferometric biosensor immunoassay for direct and label-less detection of avian influenza through whole virus capture on a planar optical waveguide is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to unique antigen-specific (hemagglutinin) antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen–antibody interaction was measured and was shown to be concentration-dependent, with detection limits as low as 0.0005 hemagglutination units per milliliter. A simple sandwich assay was shown to further increase the biosensor response. [Figure not available: see fulltext.]
ACCESSION #
26748311

 

Related Articles

  • Avian influenza surveillance reveals presence of low pathogenic avian influenza viruses in poultry during 2009-2011 in the West Bengal State, India. Pawar, Shailesh D.; Kale, Sandeep D.; Rawankar, Amol S.; Koratkar, Santosh S.; Raut, Chandrashekhar G.; Pande, Satish A.; Mullick, Jayati; Mishra, Akhilesh C. // Virology Journal;2012, Vol. 9 Issue 1, p151 

    Introduction: More than 70 outbreaks of the highly pathogenic avian influenza (HPAI) H5N1 have been reported in poultry in the western and north-eastern parts of India. Therefore, in view of the recent HPAI H5N1 outbreaks in poultry, active AI surveillance encompassing wild, resident, migratory...

  • Low Frequency of Poultry-to-Human H5N1 Virus Transmission, Southern Cambodia, 2005. Vong, Sirenda; Coghlan, Benjamin; Mardy, Sek; Holl, Davun; Heng Seng; Ly, Sovann; Miller, Megge J.; Buchy, Philippe; Froehlich, Yves; Dufourcq, Jean Baptiste; Uyeki, Timothy M.; Lim, Wilina; Sok, Touch // Emerging Infectious Diseases;Oct2006, Vol. 12 Issue 10, p1542 

    To understand transmission of avian influenza A (H5N1) virus, we conducted a retrospective survey of poultry deaths and a seroepidemiologic investigation in a Cambodian village where a 28-year-old man was infected with H5N1 virus in March 2005. Poultry surveys were conducted within a 1-km radius...

  • Receptor specificity and erythrocyte binding preferences of avian influenza viruses isolated from India. Pawar, Shailesh D.; Parkhi, Saurabh S.; Koratkar, Santosh S.; Mishra, Akhilesh C. // Virology Journal;2012, Vol. 9 Issue 1, p251 

    Introduction: Hemagglutination (HA) and hemagglutination inhibition (HI) assays are conventionally used for detection and identification of influenza viruses. HI assay is also used for detection of antibodies against influenza viruses. Primarily turkey or chicken erythrocytes [red blood cells...

  • SEROLOGICAL SURVEY OF AVIAN INFLUENZA VIRUS INFECTION OF BACKYARD CHICKENS IN BOSNIA AND HERZEGOVINA. GOLIC, B.; STEVANOVIC, O.; NEDIC, D. N.; MARIC, J. // Bulgarian Journal of Veterinary Medicine;2016, Vol. 19 Issue 4, p328 

    The aim of this research is to examine seroprevalence of avian influenza (AI) in backyard chickens from several localities in the entity of the Republic of Srpska, Bosnia and Herzegovina. Up to date there was no previous research in this topic. Our study, using ELISA test, confirmed the presence...

  • Protective measures and human antibody response during an avian influenza H7N3 outbreak in poultry in British Columbia, Canada. Skowronski, Danuta M.; Yan Li; Tweed, S. Aleina; Tam, Theresa W. S.; Petric, Martin; David, Samara T.; Marra, Fawziah; Bastien, Nathalie; Lee, Sandra W.; Krajden, Mel; Brunham, Robert C. // CMAJ: Canadian Medical Association Journal Supplement;1/2/2007, Vol. 176 Issue 1, p47 

    Background: In 2004 an outbreak of avian influenza of the H7N3 subtype occurred among poultry in British Columbia, Canada. We report compliance with recommended protective measures and associated human infections during this outbreak. Methods: We sought voluntary participation by anyone...

  • Research validates biosensor's AI detection capabilities.  // National Provisioner;Mar2010, Vol. 224 Issue 3, p32 

    The article discusses the study at Georgia Tech Research Institute (GTRI) on the potential use of an optical biosensor for the detection of avian influenza (AI) virus. It states that the concept of waveguide interferometry is used in the optical biosensor in order to determine the amount of...

  • New Portable Biosensor Detects Avian Influenza.  // USA Today Magazine;Apr2007, Vol. 135 Issue 2743, p14 

    The article features a portable biosensor for in-field screening of avian influenza virus. It was developed by a team of researchers led by professor Yanbin Li of the Division of Agriculture at the University of Arkansas in Fayetteville. It is claimed that the device detects the avian influenza...

  • A review on emerging diagnostic assay for viral detection: the case of avian influenza virus. Shojaei, Taha; Tabatabaei, Meisam; Shawky, Sherif; Salleh, Mohamad; Bald, Dirk // Molecular Biology Reports;Jan2015, Vol. 42 Issue 1, p187 

    Biotechnology-based detection systems and sensors are in use for a wide range of applications in biomedicine, including the diagnostics of viral pathogens. In this review, emerging detection systems and their applicability for diagnostics of viruses, exemplified by the case of avian influenza...

  • Serological report of influenza a (H7N9) infections among pigs in Southern China. Pei Zhou; Malin Hong; Merrill, Mary M.; Huamei He; Lingshuang Sun; Guihong Zhang // BMC Veterinary Research;2014, Vol. 10 Issue 1, p1 

    Background In 2013, a novel H7N9 avian influenza virus (AIV) was isolated from ill humans in Shanghai and Anhui Province, China. Since then, the virus has spread quickly throughout China. Previous isolation of H7N2 virus from swine suggests that additional H7 subtype AIVs may be transmitted...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics