TITLE

A study of Se-Hg antagonism in Glycine max (soybean) roots by size exclusion and reversed phase HPLC—ICPMS

AUTHOR(S)
Yathavakilla, Santha Ketavarapu V.; Caruso, Joseph A.
PUB. DATE
October 2007
SOURCE
Analytical & Bioanalytical Chemistry;Oct2007, Vol. 389 Issue 3, p715
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An attempt was made to study selenium (Se) and mercury (Hg) interactions in plants, specifically soybean ( Glycine max), by inductively coupled plasma mass spectrometric detection. Greenhouse-cultivated plants were subjected to treatment with different regimens of Se and Hg and analyzed for their metabolized species in roots, stems, leaves, pods and beans. Most of the water-soluble Hg was found to be localized in the roots in association with Se in a high molecular weight entity, as identified by size exclusion chromatography. This entity was also extracted in protein specific isolate, but it resisted enzymatic breakdown. Complete breakdown of this high molecular weight species was accomplished by acid hydrolysis. Optimization of the conditions for acid hydrolysis is discussed. Hg and Se species found in root extract were studied by ion-pairing chromatography. In a sub-study, the Se distribution pattern was found to be unaffected by the presence of Hg, but the amount of Se assimilated was found to be higher in plants coexposed to Hg.
ACCESSION #
26648540

 

Related Articles

  • Characterization of an α-amylase from sorghum ( Sorghum bicolor) obtained under optimized conditions. Adefila, O. A.; Bakare, M. K.; Adewale, I. O. // Journal of the Institute of Brewing;Jun2012, Vol. 118 Issue 1, p63 

    Sorghum malt α-amylase can compete with bacterial α-amylase in industrial applications, if sufficiently stable and produced in a large enough quantity. Conditions for maximal α-amylase production in sorghum malt and the physico-chemical properties of the α-amylase so produced are...

  • Mercury and selenium content in Bouri (Mugil cephalus) El-Sharnouby, S. A.; El-Soukkary, A.El-Soukkary; Moharram, Y. G.; Moustaffa, E. K. // Water, Air & Soil Pollution;Feb1987, Vol. 32 Issue 3/4, p455 

    No abstract available.

  • Removal of Heavy Metals Using Myriophyllum verticillatum (Whorl-Leaf Watermilfoil) in a Hydroponic System. UCER, Asiye; UYANIK, Ahmet; KUTBAY, Hamdi Guray // Ekoloji Dergisi;2013, Vol. 22 Issue 87, p1 

    The heavy metal accumulation capacity of M. verticillatum in a hydroponic system was investigated in this study. M. verticillatum was grown in a model aquatic setup consisting of aeration, lighting, and hydroponic systems for 21 days in different kinds and concentrations of heavy metal solutions...

  • A large decrease of cytosolic triosephosphate isomerase in transgenic potato roots affects the distribution of carbon in primary metabolism. Dorion, Sonia; Clendenning, Audrey; Jeukens, Julie; Salas, Joaquín; Parveen, Nanhi; Haner, Andrea; Law, R.; Force, Enrique; Rivoal, Jean // Planta;Oct2012, Vol. 236 Issue 4, p1177 

    Triosephosphate isomerase (TPI, EC 5.3.1.1) catalyzes the interconversion of dihydroxyacetone-P and glyceraldehyde 3-P in the glycolytic pathway. A constitutively expressed antisense construct for cytosolic TPI was introduced into potato ( Solanum tuberosum) using Agrobacterium rhizogenes to...

  • Biochemical Studies of Purified Extracellular Xylanases from Aspergillus versicolor. Salama, M. A.; Ismail, K. M. I.; Amany, H. A.; El-Lill, Abo; Geweely, N. S. I. // International Journal of Botany;2008, Vol. 4 Issue 1, p41 

    Fungal xylanases from alkalo-thermophilic Aspergillus versicolor that hydrolyse xylan, had been purified and characterized. The purification procedure included ammonium sulphate precipitation and chromatography on gel filteration (Sephadex G-200 and G-100) combined with ion exchange...

  • IMPROVING STABILITY OF ANAEROBIC BIOLOGICAL REACTORS USING COMPOSITE ION EXCHANGERS. Mitra, Indra N.; SenGupta, Arup K.; Kugelman, Irwin J. // Water Science & Technology;1998, Vol. 38 Issue 8/9, p369 

    Focuses on the improving stability of anaerobic biological reactors using composite ion exchangers (CIX). Evidence pertaining to long-term durability of CIX in a biological medium; Organic overloading; Heavy metal inhibition.

  • Biosorption of Hg(II) ions from synthetic wastewater using a novel biocarbon technology. Singanan, Malairajan // Environmental Engineering Research;2015, Vol. 20 Issue 1, p33 

    Mercury is a toxic pollutants present in different types of industrial effluents and is responsible for environmental pollution. Removal of Hg(II) ions from synthetic wastewater was studied using the activated biocarbon produced from the leaves of Tridax procumbens (Asteraceae). The particle...

  • Metal interactions in mice under environmental stress. García-Sevillano, M.; Jara-Biedma, R.; González-Fernández, M.; García-Barrera, T.; Gómez-Ariza, J. // BioMetals;Aug2013, Vol. 26 Issue 4, p651 

    A metallomic analytical approach based on the use of size exclusion chromatography coupled to ICP-MS has been used to obtain metal profiles related to overexpression or inhibition of metal-binding biomolecules, which is connected to exposure experiment of laboratory mice Mus musculus to toxic...

  • Studies on Biochemical Role of Accumulation of Heavy Metals in Safflower. Madaan, Nidhi; Mudgal, Varsha; Mishra, Sanjay; Srivastava, A. K.; Singh, R. B. // Open Nutraceuticals Journal;2011, Vol. 4, p199 

    Phytoremediation is the biotechnological application of plants to detoxify pollutants, and is a modern technique for environmental clean-up. Plants are ideal agents for soil and water remediation because of their unique genetic, biochemical and physiological features. Thus, the accumulation of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics