Expression and regulation of type II integral membrane protein family members in mouse male reproductive tissues

Deivendran Rengaraj; Fei Gao; Xiao-Huan Liang; Zeng-Ming Yang
April 2007
Endocrine (1355008X);Apr2007, Vol. 31 Issue 2, p193
Academic Journal
Type II Integral membrane protein (Itm2) family consists of three members, Itm2a, Itm2b and Itm2c. ITM2B has been shown to be closely related to human male reproduction. The expression and regulation of Itm2 family members in male reproductive tissues are still unknown. The aim of the present study was to examine the expression pattern and regulation of Itm2 family members in male mouse reproductive tissues during sexual maturation, castration, and busulfan treatment by in situ hybridization. During sexual maturation, a low level of Itm2a was detected in testicular interstitium on days 30–70. Itm2b expression was basally detected in the epithelium of seminiferous tubules on days 1, 5, and 10, and then the signal was transited into Leydig cells and gradually increased up to day 70. Itm2c was detected at a basal to low level in the testis during sexual maturation. Both Itm2a and Itm2c were not detected in the epididymis and vas deferens during sexual maturation. In contrast, Itm2b expression was detected in the epithelium of caput, corpus, cauda epididymis, and vas deferens from neonate to adult mice. In the caput, Itm2b expression reached the highest level on day 15 and maintained this level up to day 70. However, in corpus and cauda epididymis, the signals gradually reached a high level from days 15 to 70. In vas deferens, Itm2b gradually increased to a high level from days 25 to 70. In the castrated mice, Itm2b expression was upregulated in epididymis and vas deferens by testosterone treatments. When busulfan was used to specifically destroy the germ cells in the testis, there were no observable effects on Itm2b expression in the male reproductive organs. Our results suggested that Itm2b mRNA was differentially expressed in mouse male reproductive tissues, during sexual maturation and up-regulated by testosterone.


Related Articles

  • Detection of Cancer with Serum miRNAs on an Oligonucleotide Microarray. Lodes, Michael J.; Caraballo, Marcelo; Suciu., Dominic; Munro, Sandra; Kumar, Amit; Anderson, Brooke // PLoS ONE;2009, Vol. 4 Issue 7, p1 

    Micro RNAs (miRNAs) are a class of small, non-coding RNA species that play critical roles throughout cellular development and regulation. miRNA expression patterns taken from various tissue types often point to the cellular lineage of an individual tissue type, thereby being a more invariant...

  • Role of TSG23/Tsg23 in Spermatogenesis.  // Fertility Weekly;6/1/2009 & 6/8/2009, p5 

    The article highlights a study which identified and found the involvement of a novel testis-specific gene, TSG23/Tsg23 in spermatogenesis. The authors of the study observed that the expression of Tsg23 mRNA was decreased in a time-dependent manner in the testis of an azoospermic mouse model...

  • Prediction of a gene regulatory network linked to prostate cancer from gene expression, microRNA and clinical data. Bonnet, Eric; Michoel, Tom; Van de Peer, Yves // Bioinformatics;Sep2010, Vol. 26 Issue 18, pi638 

    Motivation: Cancer is a complex disease, triggered by mutations in multiple genes and pathways. There is a growing interest in the application of systems biology approaches to analyze various types of cancer-related data to understand the overwhelming complexity of changes induced by the...

  • Knockdown of transmembrane protein 132A by RNA interference facilitates serum starvation-induced cell death in Neuro2a cells. Oh-hashi, Kentaro; Imai, Kazuhide; Koga, Hisashi; Hirata, Yoko; Kiuchi, Kazutoshi // Molecular & Cellular Biochemistry;Sep2010, Vol. 342 Issue 1/2, p117 

    Transmembrane protein 132A (TMEM132A) is a novel GRP78 binding protein that we recently discovered. However, the biological functions of TMEM132A are merely characterized because it does not encode any known structural domains. In this study, we down regulated intrinsic TMEM132A by RNA...

  • Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer. Schostak, M.; Krause, H.; Miller, K.; Schrader, M.; Weikert, S.; Christoph, F.; Kempkensteffen, C.; Kollermann, J. // BMC Urology;2006, Vol. 6, p7 

    Background: Gene expression profiling has recently shown that the mRNA for CD24 is overexpressed in prostate carcinomas (Pca) compared to benign or normal prostate epithelial tissues. Immunohistochemical studies have reported the usefulness of anti-CD24 for detecting prostate cancer over the...

  • The Voltage-Gated Sodium Channel Nav1.8 Is Expressed in Human Sperm. Cejudo-Roman, Antonio; Pinto, Francisco M.; Subirán, Nerea; Ravina, Cristina G.; Fernández-Sánchez, Manuel; Pérez-Hernández, Natalia; Pérez, Ricardo; Pacheco, Alberto; Irazusta, Jon; Candenas, Luz // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    The role of Na+ fluxes through voltage-gated sodium channels in the regulation of sperm cell function remains poorly understood. Previously, we reported that several genes encoding voltage-gated Na+ channels were expressed in human testis and mature spermatozoa. In this study, we analyzed the...

  • Expression of VDR and VD Activating Enzymes in Male Reproductive Tract.  // Fertility Weekly;8/2/2010, p8 

    The article presents a study on the expression pattern of vitamin D receptor (VDR) and vitamin D (VD) metabolizing enzymes in human male reproductive tract specifically in the testis, epididymis, seminal vesicle, prostate, and spermatozoa. It mentions that the mechanistic research utilizes...

  • Na/H Exchanger 1 Gene Expression in Tissues of Yellow Chicken. Ning, Zhang-yong; An, Yu-fu; Qi, Wen-bao; Wang, Heng; Pan, Jia-qiang; Wu, Xin-tao; Liao, Ming // Biochemical Genetics;Apr2012, Vol. 50 Issue 3/4, p227 

    The Na/H exchanger 1 (NHE1) transmembrane protein regulates intracellular pH, cell survival, cell growth, cell differentiation and plays a critical role in the progression of some diseases, including the pathogenesis of J avian leukosis. The chicken is an ideal model to study the function of...

  • Gene Selection for Tumor Classification Using Microarray Gene Expression Data. Yendrapalli, K.; Basnet, R.; Mukkamala, S.; Sung, A. H. // World Congress on Engineering 2007 (Volume 1);2007, p290 

    In this paper we perform a t-test for significant gene expression analysis in different dimensions based on molecular profiles from microarray data, and compare several computational intelligent techniques for classification accuracy on Leukemia, Lymphoma and Prostate cancer datasets of broad...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics