Prolactin-releasing peptide (PrRP) increases prolactin responses to TRH in vitro and in vivo

Carlos Spuch; Yolanda Diz-Chaves; Diego Pérez-Tilve; Mayte Alvarez-Crespo; Federico Mallo
April 2007
Endocrine (1355008X);Apr2007, Vol. 31 Issue 2, p119
Academic Journal
The Prolactin-releasing Peptide (PrRP) is a 31-aminoacid peptide produced and secreted from the hypothalamus, and postulated to promote the prolactin release from the pituitary. However, the action of PrRP remain controversial, since it was described to have potency comparable enough to TRH, although there are many evidences that PrRP is less potent than TRH. Here we have studied the effects of PrRP alone or in combination with TRH in the prolactin levels of rat pituitary primary cell cultures in vitro and also in vivo prolactin responses in randomly cycling and estrogens-treated female rats. PrRP itself increased prolactin levels in vitro and in vivo, although in a magnitude several times lower than TRH. In vivo PrRP promotes an atypical non-peaking progressive and maintained prolactin increase. On the other hand, PrRP markedly increased the prolactin responses to TRH in vitro (10–30 fold increase) and in vivo (up to three-fold increase). In addition, FGF-2 and EGF, two important growth factors present in the pituitary, reduced the PrRP-induced prolactin increase in vitro. Taken together our results suggest that PrRP released from the hypothalamus may be relevant to modulate the circulating prolactin levels in the rat.


Related Articles

  • Satiation and stress-induced hypophagia: examining the role of hindbrain neurons expressing prolactin-releasing peptide or glucagon-like peptide 1. Maniscalco, James W.; Kreisler, Alison D.; Rinaman, Linda // Frontiers in Neuroscience;Jan2013, Vol. 7, p1 

    Neural circuits distributed within the brainstem, hypothalamus, and limbic forebrain interact to control food intake and energy balance under normal day-to-day conditions, and in response to stressful conditions under which homeostasis is threatened. Experimental studies using rats and mice have...

  • HORMONES. Tatum IV, William O.; Kaplan, Peter W.; Jallon, Pierre // Epilepsy A to Z: A Concise Encyclopedia;2009, p161 

    An encyclopedia entry for "hormones" in relation to epilepsy is presented. It explains how hormones influence epilepsy and the effect of seizures on the endocrine system. Based on animal experiments and clinical observations, it found that estrogens decrease seizure threshold and increase...

  • Effects of Prolactin-Releasing Peptide on Tuberoinfundibular Dopaminergic Neuronal Activity and Prolactin Secretion in Estrogen-Treated Female Rats. Yuan, Zung Fan; Yang, Shu-Chuan; Pan, Jenn-Tser // Journal of Biomedical Science;2002, Vol. 9 Issue 2, p112 

    Both systemic and central effects of a newly discovered prolactin (PRL)-releasing factor (PRF), prolactin-releasing peptide (PrRP), were determined in this study. Systemic injection of PrRP (1 and 10 μg/rat, i.v.) stimulated PRL secretion in ovariectomized, estrogen-treated rats similar to...

  • Hyperprolactinemia with normal serum prolactin: Its clinical significance.  // Journal of Human Reproductive Sciences;May2010, Vol. 3 Issue 2, p111 

    No abstract available.

  • Serotonin 1A Receptor Activation and Hypothermia in Humans: Lack of Evidence for a Presynaptic Mediation Blier, Pierre; Seletti, Bernard; Gilbert, Francois; Young, Simon N.; Benkelfat, Chawki // Neuropsychopharmacology;Aug2002, Vol. 27 Issue 2, p301 

    The hypothermia produced by 5-HT1A agonists had initially been claimed to be caused by the activation of cell body 5-HT1A autoreceptors resulting in decreased 5-HT transmission in laboratory animals. In order to address this issue in humans, 12 healthy volunteers underwent a dietary tryptophan...

  • Physiological roles of GPR10 and PrRP signaling. Dodd, Garron T.; Luckman, Simon M. // Frontiers in Endocrinology;Mar2013, Vol. 4, p1 

    Prolactin-releasing peptide (PrRP) was first isolated from bovine hypothalamus, and was found to act as an endogenous ligand at the G-protein-coupled receptor 10 (GPR10 or hGR3). Although originally named as it can affect the secretion of prolactin from anterior pituitary cells, the potential...

  • Evaluation of Serum Prolactin Level in Patients of Subclinical and Overt Hypothyroidism. GOEL, PARUL; KAHKASHA; NARANG, SHVETA; GUPTA, BHARAT K.; GOEL, KAPIL // Journal of Clinical & Diagnostic Research;Jan2015, Vol. 9 Issue 1, p15 

    Background: Prolactin secretion is controlled by prolactin inhibitor factor that is secreted from hypothalamus; factors like vasoactive inhibitory peptide (VIP) and thyroid releasing hormone (TRH) lead to increase in prolactin secretion. Hyperprolactinemia is a common condition that can result...

  • Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats. Ratnayake, Sunil; Lewandowski, Paul // Lipids in Health & Disease;2010, Vol. 9, p13 

    It has been consistently reported that vegetable oils including canola oil have a life shortening effect in Stroke- Prone Spontaneously Hypertensive Rats (SHRSP) and this toxic effect is not due to the fatty acid composition of the oil. Although it is possible that the phytosterol content or...

  • Kickstarting P53.  // BioWorld Today;9/3/2013, Vol. 24 Issue 168, Special section p1 

    The article offers brief information on a report by scientists from biopharmaceutical company Aileron Therapeutics Inc. that its stapled peptide, ATSP-704, can reactivate P53 signaling in cell culture to inhibit the proteins MDM2 and MDMX which regulate tumor suppressor.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics