TITLE

Carrageenan Reduces Bone Morphogenetic Protein-4 (BMP4) and Activates the Wnt/β-Catenin Pathway in Normal Human Colonocytes

AUTHOR(S)
Sumit Bhattacharyya; Alip Borthakur; Pradeep K. Dudeja
PUB. DATE
October 2007
SOURCE
Digestive Diseases & Sciences;Oct2007, Vol. 52 Issue 10, p2766
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  Carrageenans are highly sulfated polysaccharides that are widely used as food additives in the Western diet, in order to improve the texture of processed foods. Although native and degraded carrageenans induce colonic ulcerations, polyps, and colorectal tumors in animal models, very little is known about the effects of carrageenan on human colonocytes. We evaluated effects of λ-carrageenan (λCGN) on the normal human colonocyte cell line NCM460, using a concentration of 1 μg/ml, about less than one tenth the average daily exposure to carrageenan in the Western diet. We measured secreted bone morphogenetic protein-4 (BMP4) in spent media and quantified its expression by quantitative RT-PCR. Wnt-related genes were measured by an oligonucleotide array. Cellular β-catenin was quantified by ELISA. We found a marked decline in secreted BMP4 (P
ACCESSION #
26588661

 

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