TITLE

Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

AUTHOR(S)
Madhu S. Budamagunta; John Hess; Paul Fitzgerald; John Voss
PUB. DATE
May 2007
SOURCE
Cell Biochemistry & Biophysics;May2007, Vol. 48 Issue 1, p45
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
ACCESSION #
26420181

 

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