Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

Madhu S. Budamagunta; John Hess; Paul Fitzgerald; John Voss
May 2007
Cell Biochemistry & Biophysics;May2007, Vol. 48 Issue 1, p45
Academic Journal
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.


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