Production and Partial Purification of Cellulase by Aspergillus niger and A. fumigatus Fermented in Coir waste and Sawdust

Immanuel, G.; Bhagavath, C. M. Akila; Raj, P. Iyappa; Esakkiraj, P.; Palavesam, A.
January 2007
Internet Journal of Microbiology;Jan2007, Vol. 3 Issue 1, p6
Academic Journal
It was the goal to investigate the cellulase enzyme production ability of fungal strains such as Aspergillus niger and A. fumigatus against the lignocellulosic bio waste like coir waste and saw dust at varying environmental parameters of pH (5-9) and temperature (20-50°C). The enzyme production was analysed individually by Dinitrosalicylic acid (DNS) and Filter paper assay (FPA) methods. In the DNS method, high level of enzyme production was achieved at pH 5 (0.198 IU ml[sup -1]) and pH 6 (0.052 IU ml[sup -1]) by A. fumigatus using coir waste and saw dust as substrates respectively. Regarding temperature, at 40°C maximum enzyme production (0.290 IU ml[sup -1]) was recorded by A. fumigatus in coir waste and at the same time 50°C was optimum for maximum (0.274 IU ml[sup -1]) enzyme production by A. niger in saw dust waste. In FPA method, high level of enzyme production was obtained at pH7 by A. fumigatus (0.292 IU ml[sup -1]) and pH6 by A. niger (0.262 IU ml[sup -1]) when coir waste and saw dust used as substrates respectively. Similarly, at varying temperature, high level of enzyme production was obtained at 40°C by both organisms using both substrates. The partial purification of the cellulase enzyme revealed that, A. niger grown in coir waste supplemented medium had two protein bands with the molecular weight of 36 and 23kDa respectively, similarly A. fumigatus grown in the same medium also had two protein bands with molecular weight of 32 and 21kDa respectively.


Related Articles

  • Purification and characterization of a nitrilase from Aspergillus niger K10.  // Applied Microbiology & Biotechnology;Dec2006, Vol. 73 Issue 3, p567 

    Aspergillus niger K10 cultivated on 2-cyanopyridine produced high levels of an intracellular nitrilase, which was partially purified {18.6-fold) with a 24% yield. The N-terminal amino acid sequence of the enzyme was highly homologous with that of a putative nitrilase from Aspergillus fumigatus...

  • Maximum Saccharification of Cellulose Complex by an Enzyme Cocktail Supplemented with Cellulase from Newly Isolated Aspergillus fumigatus ECU0811. Wang, Dan; Sun, Jie; Yu, Hui-Lei; Li, Chun-Xiu; Bao, Jie; Xu, Jian-He // Applied Biochemistry & Biotechnology;Jan2012, Vol. 166 Issue 1, p176 

    Either the natural biodegradation process or the industrial hydrolytic process requires synergistic interactions between various cellulases. However, it is sometimes impeded by low hydrolytic rate of existing cellulases and the lack of accessory enzymes. Herein, the ability of a commercial...

  • A KINETIC STUDY ON CELLULASE ENZYMES FROM ASPERGILLUS NIGER. ARTI, SAXENA; ANURAG, SAXENA; SARIKA, YADAV // International Journal of Pharma & Bio Sciences;Jul-Sep2011, Vol. 2 Issue 3, pB.36 

    A physico-chemical study was made on cellulase enzymes from a highly cellulolytic fungus Aspergillus niger. Various kinetic parameters were estimated by making use of potent fungicides, viz. m-dinitrobenzene and pentachlorophenol. The enzyme showed maximum activity at pH 5.0 and temp. 55o C. The...

  • Isolation and Characterization of Cellulase Enzyme from Aspergillus sps. Devi, D. Kanchana; Hemashenpagam, N. // IUP Journal of Biotechnology;Mar2010, Vol. 4 Issue 1, p58 

    The cellulase enzyme producing fungus Aspergillus niger was isolated and identified from hilly irrigated soil. The substrate concentration, pH, incubation temperature and carbon sources of the czapekdox medium were varied and maximum production of cellulase was studied. Cellulase activity was...

  • Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi. Gautam, S. P.; Bundela, P. S.; Pandey, A. K.; Khan, Jamaluddin; Awasthi, M. K.; Sarsaiya, S. // Biotechnology Research International;2011, p1 

    The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus...

  • Study of the Enzymatic Hydrolysis of Alkaline-Pretreated Rice Straw Using Cellulase of Various Sources and Compositions. Anwar, Nadiem; Widjaja, Arief; Winardi, Sugeng // International Review of Chemical Engineering - Rapid Communicati;Mar2011, Vol. 3 Issue 2, p272 

    The study of the enzymatic hydrolysis of alkaline-pretreated rice straw was conducted using mixture of cellulases produced from Trichoderma reesei and from Aspergillus niger. 2 % NaOH was found effective in reducing the lignin content in rice straw as evidenced from quantitative data and...

  • Development of complex enzymatic preparations of pactinases and celulases for sugar beet marc digestion. Bushina, E.; Rozhkova, A.; Zorov, I.; Satrutdinov, A.; Bekkarevich, A.; Koshelev, A.; Okunev, O.; Sinitsyn, A. // Applied Biochemistry & Microbiology;Sep2012, Vol. 48 Issue 5, p493 

    Complex enzymatic preparations demonstrating activities homologous to pectinlyase A and heterologous to endo-1,4-β-glucanase from Penicilliumverruculosum and β-glycosidase from Aspergillusniger have been obtained on the basis of recombinant strains of the fungus Penicilliumcanescens. Two...

  • Influence of exogenous fibrolytic enzymes on in vitro and in sacco degradation of forages for ruminants. Gallardo, Ismael; Bárcena, Ricardo; Pinos-Rodríguez, Juan Manuel; Cobos, Mario; Carreón, Lorenzo; Ortega, María Esther // Italian Journal of Animal Science;Feb2010, Vol. 9 Issue 1, p34 

    An in vitro assay was carried out to evaluate the effects of exogenous fibrolytic enzymes (1, 2, 3 and 4 g/kg DM) powder preparation containing xylanase and cellulase from Aspergillus niger and Trichoderma viride on DM, NDF and ADF degradation of alfalfa hay, corn silage, corn stover, elephant...

  • Microbial Spoilage of Irvingia Kernels in Benin City, Nigeria. Iyayi, Ikhatua Matilda; Adewumi, Egharevba Rachael Kehinde; Nassang, Asa'a Lemawah // Archives of Applied Science Research;2010, Vol. 2 Issue 5, p168 

    The spoilage of kernels (seeds) of two Irvingia species i.e. I. gabonensis and I. wombulu was investigated in Benin City, Nigeria. Samples obtained from nine markets in three local government areas (Oredo,lkpoba-Okha and Egor) within the Benin Metropolis, were brought to the laboratory for...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics