TITLE

Stable carbon isotope ratios of methyl-branched fatty acids are different to those of straight-chain fatty acids in dairy products

AUTHOR(S)
Vetter, Walter; Gaul, Simon; Thurnhofer, Saskia; Mayer, Kathrin
PUB. DATE
September 2007
SOURCE
Analytical & Bioanalytical Chemistry;Sep2007, Vol. 389 Issue 2, p597
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Methyl-branched fatty acids (MBFAs) are the dominant form of fatty acid found in many bacteria. They are also found at low levels in a range of foodstuffs, where their presence has been linked to bacterial sources. In this study we evaluated the potential of compound-specific isotope analysis to obtain insights into the stable carbon isotope ratios (δ13C values in ‰) of individual MBFAs and to compare them to the stable carbon isotope ratios of straight-chain fatty acids in food. Due to their low abundance in foodstuffs, the MBFAs were enriched prior to gas chromatography coupled to isotope ratio mass spectrometric (GC–IRMS) analysis. After transesterification, urea complexation was used to suppress the 16:0 and 18:0 methyl esters that were dominant in the samples. Following that, silver-ion high performance liquid chromatography was used to separate the saturated from the unsaturated fatty acids. The resulting solutions of saturated fatty acids obtained from suet, goat’s milk, butter, and human milk were studied by GC–IRMS. The δ13C values of fatty acids with 12–17 carbons ranged from −25.4‰ to −37.6‰. In all samples, MBFAs were most depleted in carbon-13, followed by the odd-chain fatty acids 15:0 and 17:0. 14:0 and 16:0 contained the highest proportions of carbon-13. The results from this study illustrate that MBFAs have distinctive δ13C values and must originate from other sources and/or from very different substrates. These measurements support the initial hypothesis that δ13C values can be used to attribute MBFAs to particular sources.
ACCESSION #
26340091

 

Related Articles

  • Type Composition of Triglyceride Seed Oils. III. Triglycerides from Certain Plants of the Ranunculaceae Family. Deineka, V. I.; Sirotin, A. A.; N. V. Ospishcheva // Chemistry of Natural Compounds;May/Jun2004, Vol. 40 Issue 3, p295 

    Focuses on the examination of triglycerides from plant oils in Belgorod, Russia. Use of reversed-phase HPLC in the study; Results of the seed oils collected from plants; Establishment of the fatty acid composition by traditional methods.

  • High Performance Liquid Chromatography Determination of Fatty Acids in Drying Oils Following Lipase Action. Tarola, A.M.; Girelli, A.M.; Lorusso, S. // Journal of Chromatographic Science;Apr2012, Vol. 50 Issue 4, p294 

    This paper describes a quantitative analytical procedure to determine the fatty acid composition in drying oils like linseed, walnut and poppy seed. The procedure required the enzymatic hydrolysis of the oil triacylglycerol families by the action of Candida rugosa lipase. The fatty acids (FFAs)...

  • Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California. Band-Schmidt, C.; Martínez-López, A.; Bustillos-Guzmán, J.; Carreón-Palau, L.; Morquecho, L.; Olguín-Monroy, N.; Zenteno-Savín, T.; Mendoza-Flores, A.; González-Acosta, B.; Hernández-Sandoval, F.; Tomas, C. // Hydrobiologia;Aug2012, Vol. 693 Issue 1, p81 

    Strains of raphidophytes from different regions of the Gulf of California were established for identification and biochemical description. Raphidophyte in our coasts have been traditionally identified based on microscopic observations, a biochemical analyses of strains present in our coast is...

  • Quantitation of Omega Fatty Acids by HPLC and Charged Aerosol Detection. Plante, Marc; Bailey, Bruce; Acworth, Ian // LC-GC North America;Feb2012 Supplement, Vol. 30, p34 

    An abstract of the study "Quantitation of Omega Fatty Acids by HPLC and Charged Aerosol Detection," by Marc Plante, Bruce Bailey, and Ian Acworth is presented.

  • Isoflavone Content of Soybean Cultivars from Maturity Group 0 to VI Grown in Northern and Southern China. Zhang, Jingying; Ge, Yinan; Han, Fenxia; Li, Bin; Yan, Shurong; Sun, Junming; Wang, Lianzheng // Journal of the American Oil Chemists' Society (JAOCS);Jun2014, Vol. 91 Issue 6, p1019 

    Soybean isoflavone content has long been considered to be a desirable trait to target in selection programs for their contribution to human health and plant defense systems. The objective of this study was to determine isoflavone concentrations of various soybean cultivars from maturity groups 0...

  • High-performance liquid chromatographic assay with fluorescence detection for the evaluation of inhibitors against fatty acid amide hydrolase. Forster, Laura; Elfringhoff, Alwine Schulze; Lehr, Matthias // Analytical & Bioanalytical Chemistry;Jul2009, Vol. 394 Issue 6, p1679 

    A fluorescent assay for the evaluation of inhibitors of fatty acid amide hydrolase (FAAH) is described. Microsomes from rat brain served as enzyme source. N-(2-Hydroxyethyl)-4-pyren-1-ylbutanamide was designed and synthesized as novel fluorogenic substrate. For substrate solubilization, Triton...

  • Application of Multivariate Analysis for Detection of Crude Palm Oil Adulteration through Fatty Acid Composition and Triacylglycerol Profile. Inthiram, Anand Kumar; Mirhosseini, Hamed; Chin Ping Tan; Mohamad, Rosfarizan; Oi Ming Lai // Pertanika Journal of Tropical Agricultural Science;Aug2015, Vol. 38 Issue 3, p389 

    This study focused on developing a reliable procedure for the identification of the adulteration of crude palm oil (CPO) by blending sludge oils (SO) and used vegetable oils (UVO) ranging from 1 to 20% (v/v). Fatty acids methyl esters (FAME) and Triacylglycerol composition consisting of all...

  • Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS. CAIDAN, REZENG; CAIRANG, LIMAO; LIUBIN; YOURUI, SUO // Asian Journal of Chemistry;Apr2013, Vol. 25 Issue 4, p1866 

    The aim of this study was to optimize the microwave extraction conditions of Rubus niveus Thunb. oil from Qinghai-Tibetan plateau via Box-Behnken design of RSM and then analyze the composition of oil by HPLC-MS/MS using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl- 4-toluene-sulfonate as labeling...

  • Occurrence of Aflatoxin M1 in Pasteurized Milk of the School Milk Project in Thailand. RUANGWISES, SUTHEP; RUANGWISES, NONGLUCK // Journal of Food Protection;Aug2009, Vol. 72 Issue 8, p1761 

    Concentrations of aflatoxin M1 (AFM1) were determined in 150 pasteurized milk samples from the School Milk Project in Thailand. Milk samples were collected from 50 schools in the Central region of Thailand in three seasons: summer (May to June 2006), the rainy season (August to September 2006),...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics