TITLE

Enzymatic detection of glucose using fluoride-selective electrodes with polymeric membranes

AUTHOR(S)
Górski, Łukasz; Klimaszewska, Dorota; Pietrzak, Mariusz; Malinowska, Elżbieta
PUB. DATE
September 2007
SOURCE
Analytical & Bioanalytical Chemistry;Sep2007, Vol. 389 Issue 2, p533
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The feasibility of using polymeric membrane fluoride-selective electrodes based on zirconium(IV) 5,10,15,20-tetraphenylporphyrin as a detector in a flow-injection analysis (FIA) system for glucose determination was examined. The optimization of enzymatic reactions, FIA system configuration and enzyme-immobilization process was performed. It was shown that the resulting flow-injection system exhibits good working parameters, such as reproducibility, linear range of glucose concentration (3 × 10−3–10−1 M), sampling rate (60 samples per minute) and lifetime (over 1 month). The performance of the polymeric membrane electrode was similar to that of a crystalline LaF3 electrode. The results of glucose determination in synthetic samples with the proposed system show good agreement with real glucose concentrations.
ACCESSION #
26340069

 

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