Chromatographic fingerprint analysis for evaluation of Ginkgo biloba products

Pei Chen; Ozcan, Mustafa; Harnly, James
September 2007
Analytical & Bioanalytical Chemistry;Sep2007, Vol. 389 Issue 1, p251
Academic Journal
The flavonoids and the terpene lactones are regarded as the two main active components of Ginkgo biloba that affect human health. In the work discussed in this paper, two analytical methods for the characterization of G. biloba authentic materials and commercial products, an LC–UV chromatographic fingerprinting method and a traditional flavonol quantification method, were compared. The traditional method was used to determine the total flavonol content (as glycosides) after acid hydrolysis. The fingerprinting method examined the chromatographic profiles of methanol–water extracts using chemometric methods. The traditional method showed that all the commercial products met the current voluntary standard of 24% flavonols by weight. The chromatographic fingerprinting method revealed significant variations in the commercial products with regard to the relative concentration of individual flavonols.


Related Articles

  • Rapid Microwave-Assisted Hydrolysis for Determination of Ginkgo Flavonol Glycosides in Extracts of Ginkgo biloba Leaves. J. Xiping Chen; Yangde Zhang // Journal of Chromatographic Science;Feb2008, Vol. 46 Issue 2, p117 

    A rapid microwave-assisted hydrolysis (MAH) method is presented for the sample pretreatment of the determination of ginkgo flavonol glycosides in extracts of Ginkgo biloba L. (EGb). By this method, flavonol glycosides can be completely hydrolyzed within 2 min. After investigating the effects of...

  • Study debunks ginkgo as memory enhancer.  // Asian Pacific Post;3/6/2008, p16 

    The article focuses on a study on the effects of ginkgo biloba extracts on memory. The study found that the gingko biloba does not really help enhance a person's memory. Found mostly in China, the extract of the plant's leaves contains flavonoid glycosides and terpenoids, and was at one time...

  • Ginkgo biloba Extract Increases Ocular Blood Flow Velocity. Chung, Hak Sung; Harris, Alon; Kristinsson, Johannes Kari; Ciulla, Thomas A.; Kagemann, Carol; Ritch, Robert // Journal of Ocular Pharmacology & Therapeutics;Jun99, Vol. 15 Issue 3, p233 

    Evaluates the effect of Ginkgo biloba extract (GBE) on ocular blood flow in normal volunteers. Flow diagram of a randomized controlled trial with crossover; Hemodynamic responses to placebo and GBE measured by Color Doppler imaging; Role of flavonoids and terpene lactones on GBE.

  • Sesquiterpene lactones and flavonoids from Centaurea foucauldiana. Bensouici, C.; Kabouche, A.; Kabouche, Z.; Touzani, R.; Bruneau, C. // Chemistry of Natural Compounds;Jul2012, Vol. 48 Issue 3, p510 

    The article presents a study which evaluates the sesquiterpene lactones and flavonoids of the endemic species Centaurea foucauldiana Maire. The study used nuclear magnetic resonance (NMR), high technique experiments, and column chromatography. Results show that sesquiterpene lactones are...

  • Analysis of Flavonol Aglycones and Terpenelactones in Ginkgo biloba Extract: A Comparison of High-Performance Thin-Layer Chromatography and Column High-Performance Liquid Chromatography. Gray, Dean E.; Messer, Dale; Porter, Andrew; Hefner, Brian; Logan, Dama; Harris, Roger K.; Clark, Alice P.; Algaler, Joseph A.; Overstreet, J. Diane; Smith, Cynthia S. // Journal of AOAC International;Sep/Oct2007, Vol. 90 Issue 5, p1203 

    The article reports findings of method comparisons for the terpenelactones and flavonol aglycones by column high-performance liquid chromatography (HPLC) with light scattering and ultraviolet detection, and high-performance thin-layer chromatography (HPLTC) with a scanning densitometer. The...

  • Preliminary Phytochemical and Physicochemical Characterization of Gynura segetum (Lour) Merr (Compositae) Leaf. Lay Jing Seow; Hooi Kheng Beh; Sadikun, Amirin; Asmawi, Mohd Zaini // Tropical Journal of Pharmaceutical Research;Oct2013, Vol. 12 Issue 5, p777 

    Purpose: To carry out phytochemical and physicochemical studies on the leaves of Gynura segetum. Methods: Preliminary phytochemical and physicochemical studies using chromatographic techniques, were carried out, Parameters evaluated include ash value, loss on drying and extractive value, amongst...

  • Structure of Samferine. Eshbakova, K. A.; Saidkhodzhaev, A. I. // Chemistry of Natural Compounds;Mar/Apr2004, Vol. 40 Issue 2, p194 

    Presents systematic research on terpenoids of plants in the Ferula L. genus. Isolation of Ferula samarcandica from roots by chromatographic separation over silica gel with elution by benzine:ethylacetate; Molecular structure of samferine; Alkaline hydrolysis; Chemical shift of the angular methyl.

  • Flavonoid glucosides from Centaurea sphaerocephala. Bentamene, A.; Boucheham, R.; Baz, M.; Benayache, S.; Creche, J.; Benayache, F. // Chemistry of Natural Compounds;Jul2010, Vol. 46 Issue 3, p452 

    The article presents a study which examines the n-butanol soluble composition of the aqueous-methanol extract of Centaurea sphaerocephala that were gathered during the flowering season in May 2003 at El Kala in East of Algeria. It states that the study involves filtration of the macerated and...

  • Flavonoid glycosides from flowers of Sisymbrium officinale and Diplotaxis muralis growing in Georgia. Alaniya, M.; Kavtaradze, N.; Skhirtladze, A.; Sutiashvili, M.; Kemertelidze, E. // Chemistry of Natural Compounds;May2012, Vol. 48 Issue 2, p315 

    The article offers information on a chemical study conducted on the flowers of Sisymbrium officinale Scop. and Diplotaxis muralis growing in Georgia. It states that the study used column chromatography, mass spectrometry and alkaline hydrolysis to analyze the chemical composition of flowers....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics