TITLE

Relationship between sperm DNA damage, induced acrosome reaction and viability in ICSI patients

AUTHOR(S)
Ozmen, B.; Caglar, G. S.; Koster, F.; Schopper, B.; Diedrich, K.; Al-Hasani, S.
PUB. DATE
August 2007
SOURCE
Reproductive BioMedicine Online (Reproductive Healthcare Limited;Aug2007, Vol. 15 Issue 2, p208
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The DNA damage in human spermatozoa is a relevant predictor of prognosis in male infertility, whereby increased sperm DNA damage impairs the outcomes of artificial reproduction. Theoretically, DNA damage should alter the special cellular functions of human spermatozoa, and lead to diminished acrosome reaction with reduced fertilization rates. Nevertheless, intracytoplasmic sperm injection (ICSI) has been reported to alleviate such negative outcomes due to DNA damage. This study investigated the relationship between DNA fragmentation and acrosome reaction as well as viability in ICSI patients. The study enrolled 42 men undergoing ICSI due to poor sperm parameters. The DNA fragmentation indexes (DFI) were 4--10% in 38% of the cases, and ≥ 10% in 19% of the cases. The results of both acrosome reaction and viability assays showed negative correlations with DFI values in all cases and especially in cases with fertilization rates <60% (P < 0.05). However, such correlations were not found in cases with fertilization rates >60%. There were no live deliveries in patients with high DFI levels (>10%). In conclusion, negative correlations were identified between increased DNA damage, and acrosome reaction and/or viability of human spermatozoa, especially in cases with reduced fertilization rates.
ACCESSION #
26183962

 

Related Articles

  • In vitro Mercury Exposure on Spermatozoa from Normospermic Individuals. Arabi, M.; Heydarnejad, M. S. // Pakistan Journal of Biological Sciences;2007, Vol. 10 Issue 15, p2448 

    Epidemiological evidence suggests that exposure to industrial metal aerosols is detrimental to the male reproductive system. Oxidative stress has been identified as a crucial factor leading to male factor infertility largely due to peroxidative damage to the sperm cell membrane. The objectives...

  • DNA fragmentation in mammalian spermatozoa and its relationship with male infertility. Vaseghi-Dodaran H.; Daghighkia H.; Atshan M.; Sobhani A.; Sobhani A. G. H. // Iranian Journal of Reproductive Medicine;Apr2015 Supplement, p82 

    Introduction: It has been suggested that altered nuclear chromatin structure or damaged DNA in spermatozoa is implicated as a possible cause of increased infertility in males. Since the first reports on sperm DNA integrity, this subject has become the focus of numerous studies. Up to 8% of...

  • Sperm chromatin dispersion test in the assessment of DNA fragmentation and aneuploidy in human spermatozoa. Balasuriya, A.; Speyer, B.; Serhal, P.; Doshi, A.; Harper, J. C. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;May2011, Vol. 22 Issue 5, p428 

    Sperm DNA damage is thought to be increased in men with male factor infertility. Previous studies suggest a correlation between sperm DNA fragmentation and aneuploidy. The sperm chromatin dispersion (SCD) test was modified to produce the Halosperm Kit. The SCD-fluorescent in-situ hybridization...

  • A comparison of DNA damage in testicular and proximal epididymal spermatozoa in obstructive azoospermia. Steele, E. Kristine; McClure, Neil; Maxwell, Robert J.; Lewis, Sheena E.M. // MHR: Basic Science of Reproductive Medicine;Sep99, Vol. 5 Issue 9 

    Testicular and epididymal spermatozoa are used routinely for intracytoplasmic sperm injection (ICSI) to treat men with obstructive azoospermia. Little is known of the effects of obstruction and stasis on the DNA of these spermatozoa, particularly in the epididymis where spermatozoa have been...

  • Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Ermanno Greco; Filomena Scarselli; Marcello Iacobelli; Laura Rienzi; Filippo Ubaldi; Susanna Ferrero; Giorgio Franco; Nazareno Anniballo; Carmen Mendoza; Jan Tesarik // Human Reproduction;Jan2005, Vol. 20 Issue 1, p226 

    BACKGROUND: Sperm DNA damage (fragmentation) is a recently discovered cause of male infertility for which no efficient treatment has yet been found. Previous findings have suggested that clinically relevant sperm DNA damage may occur at the post-testicular level. This study was undertaken to...

  • The effects of male age on sperm DNA damage in an infertile population. Vagnini, L.; Baruffi, R. L. R.; Mauri, A. L.; Petersen, C. G.; Massaro, F. C.; Pontes, A.; Oliveira, J. B. A.; Franco Jr, J. G. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Nov2007, Vol. 15 Issue 5, p514 

    The objective was to investigate the influence of age on sperm DNA damage. Semen samples were collected from 508 men in an unselected group of couples attending infertility investigation and treatment. DNA fragmentation in spermatozoa was measured by TdT (terminal deoxynucleotidyl...

  • The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Robinson, Lynne; Gallos, Ioannis D.; Conner, Sarah J.; Rajkhowa, Madhurima; Miller, David; Lewis, Sheena; Kirkman-Brown, Jackson; Coomarasamy, Arri // Human Reproduction;Oct2012, Vol. 27 Issue 10, p2908 

    STUDY QUESTION Is there an association between high levels of sperm DNA damage and miscarriage? SUMMARY ANSWER Miscarriage rates are positively correlated with sperm DNA damage levels. WHAT IS KNOWN ALREADY Most ejaculates contain a subpopulation of sperm with DNA damage, also referred to as DNA...

  • MACS Improves IUI Outcome for Patients with Unexplained Infertility.  // Fertility Weekly;6/14/2010, p8 

    The article reports on the use of magnetic-activated cell sorting (MACS) which reduces the level of apoptopic markers in sperm and results in better fertilization rates for couples with unexplained infertility (UI) in a research in Taiwan. It states that MACS used for sperm preparation reduces...

  • Astaxanthin Improves Human Sperm Capacitation by Inducing Lyn Displacement and Activation. Andrisani, Alessandra; Donà, Gabriella; Tibaldi, Elena; Brunati, Anna Maria; Sabbadin, Chiara; Armanini, Decio; Alvisi, Gualtiero; Gizzo, Salvatore; Ambrosini, Guido; Ragazzi, Eugenio; Bordin, Luciana // Marine Drugs;2015, Vol. 13 Issue 9, p5533 

    Astaxanthin (Asta), a photo-protective red pigment of the carotenoid family, is known for its multiple beneficial properties. In this study, the effects of Asta on isolated human sperm were evaluated. Capacitation involves a series of transformations to let sperm acquire the correct features for...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics