TITLE

Early Wound Irrigation Improves the Ability to Remove Bacteria

AUTHOR(S)
Owens, Brett D.; Wenke, Joseph C.
PUB. DATE
August 2007
SOURCE
Journal of Bone & Joint Surgery, American Volume;Aug2007, Vol. 89-A Issue 8, p1723
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Although most surgeons prefer to treat contaminated wounds as soon as possible, the effect of timing on the ability of irrigation to reduce the amount of bacteria in a wound is not fully known. We evaluated the effect of different delays in irrigation on bacterial removal in an animal model. Methods: A complex musculoskeletal wound was created in the proximal part of the leg of goats. The wound was contaminated with Pseudomonas aeruginosa (lux) bacteria, genetically modified to emit photons, in order to allow for quantitative analysis of bacterial concentration with a photon-counting camera system. The contaminated wounds were closed, and wound irrigation was performed with 6 L of normal saline solution by means of pulsatile lavage after the assigned time-intervals of three, six, and twelve hours. Images were made before and after treatment. Relative luminescent units and clearance ratios were obtained and calculated for each wound. Results: Earlier wound irrigation resulted in superior bacterial removal in our model. Irrigation resulted in a 70% ± 2%, 52% ± 3%, and 37% ± 4% reduction in bacterial counts from the pre-irrigation level at three, six, and twelve hours, respectively. The clearance ratios were significantly different at all time-points (p < 0.004). Conclusions: Earlier irrigation in our contaminated wound model resulted in superior bacterial removal. Clinical Relevance: While the actual bacterial counts necessary to establish a wound infection in humans is unknown, early irrigation of the contaminated wound is recommended for the prevention of infection.
ACCESSION #
26180958

 

Related Articles

  • Gagged killer bugs stay sweet. Johnston, Nicole // New Scientist;11/06/99, Vol. 164 Issue 2211, p24 

    Reports on the efforts of scientists to prevent bacteria from communicating with one another and therefore stopping their spread. Studies done on the bacteria Pseudomonas aeruginosa which can infect wounds and kill patients with severe burns; Use of genes which produce specific enzymes;...

  • Membrane vesicles traffic signals and facilitate group activities in a prokaryote. Mashburn, Lauren M.; Whiteley, Marvin // Nature;9/15/2005, Vol. 437 Issue 7057, p422 

    Many bacteria use extracellular signals to communicate and coordinate social activities, a process referred to as quorum sensing. Many quorum signals have significant hydrophobic character, and how these signals are trafficked between bacteria within a population is not understood. Here we show...

  • Wider Access to Genotypic Space Facilitates Loss of Cooperation in a Bacterial Mutator. Harrison, Freya; Buckling, Angus // PLoS ONE;2011, Vol. 6 Issue 2, p1 

    Understanding the ecological, evolutionary and genetic factors that affect the expression of cooperative behaviours is a topic of wide biological significance. On a practical level, this field of research is useful because many pathogenic microbes rely on the cooperative production of public...

  • The Crystal Structure of OprG from Pseudomonas aeruginosa, a Potential Channel for Transport of Hydrophobic Molecules across the Outer Membrane. Touw, Debra S.; Patel, Dimki R.; van den Berg, Bert // PLoS ONE;2010, Vol. 5 Issue 11, p1 

    Background: The outer membrane (OM) of Gram-negative bacteria provides a barrier to the passage of hydrophobic and hydrophilic compounds into the cell. The OM has embedded proteins that serve important functions in signal transduction and in the transport of molecules into the periplasm. The...

  • Bacterial Quorum Interrupted.  // PharmaGenomics;Mar/Apr2003, Vol. 3 Issue 3, p10 

    Reports on the discovery of a new drug compound that can knock out the quorum-sensing genes in Pseudomonas aeruginosa in Buffalo, New York as of March 2003. Function of the quorum sensing system; Characteristics of the bacteria; Approach of the compound to the treatment of bacterial cells.

  • Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Park, Pyong Woo; Pier, Gerald B.; Hinkes, Michael T.; Bernfield, Merton // Nature;5/3/2001, Vol. 411 Issue 6833, p98 

    Presents a study which showed that the shedding of syndecan-1 by Pseudomonas aeruginosa enhanced bacterial virulence in newborn mice. Research methodology; Results and discussion; Conclusions.

  • Pangenome-wide and molecular evolution analyses of the Pseudomonas aeruginosa species. Mosquera-Rendón, Jeanneth; Rada-Bravo, Ana M.; Cárdenas-Brito, Sonia; Corredor, Mauricio; Restrepo-Pineda, Eliana; Benítez-Páez, Alfonso // BMC Genomics;1/12/2016, Vol. 17, p1 

    Background: Drug treatments and vaccine designs against the opportunistic human pathogen Pseudomonas aeruginosa have multiple issues, all associated with the diverse genetic traits present in this pathogen, ranging from multi-drug resistant genes to the molecular machinery for the biosynthesis...

  • Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026. Kabir, Ahmad Humayan; Roy, Anindya Ghosh; Alam, Mohammad Firoz; Islam, Rafiul // Notulae Scientia Biologicae;2010, Vol. 2 Issue 4, p72 

    Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabadus nematophila were cultured in...

  • PcrH of Pseudomonas aeruginosa Is Essential for Secretion and Assembly of the Type III Translocon. Broms, Jeanette E.; Forslund, Anna-Lena; Forsberg, Ake; Francis, Matthew S. // Journal of Infectious Diseases;12/15/2003, Vol. 188 Issue 12, p1909 

    Discusses the importance of the PcrH of Pseudomonas aeruginosa for the secretion and assembly of the Type III translocon. Background on the type III secretion system of P. aeruginosa that translocates antihost effectors into an infected eukaryotic cell; Impact of the absence of PcrH on the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics