Genome Identity and diversity Study in Gir and Kankrej (Bos indicus) Cattle Breeds using RAPD Fingerprints

Joshi, Jarina; Patel, R. K.; Singh, K. M.; Soni, K. J.; Chauhan, J. B.; Rank, D. N.; Joshi, C. G.; Rao, K. R. S. Sambasiva
September 2007
Biotechnology;2007, Vol. 6 Issue 3, p322
Academic Journal
RAPD-PCR techniques were applied to study the genetic identity and diversity in two Indian cattle breeds Kankrej and Gir with a battery of 16 decamer primers. The analysis was carried out by using Band Frequency (BF), Band Sharing Frequency (BSF), Genetic Identity (GI), Genetic Distance (GD) and Mean Average Percentage Deference (MAPD). RAPD pattern revealed polymorphism for 163 loci out of 196 loci (83.16%) in Gir and Kankrej breeds. The present study showed that within breed similarity in both the breeds was observed to be greater as compared to similarity between breeds. Within breed genetic similarity was higher in Gir cattle than Kankrej cattle. Primer KMS6 was able to resolve a product of 388 bp that was seen in 11 out of 12 Gir animals. Similarly primer KMS2 revealed Kankrej specific amplicon of 2274 bp that was seen in 11 out of 12 Kankrej animals. The estimate of band sharing frequency between breeds was highest (0.900) with the primer KMS6 and the lowest (0.557) with the primer KMS11. The band sharing frequency pooled over the primer was 0.738±0.2008 between these two breeds. The highest genetic identity estimate (0.943) between the two breeds was obtained with primer KMS3 and the lowest (0.696) with primer KMS10. Similarly the highest genetic distance estimate (0.20) between the two breeds was obtained with KMS2 and the lowest (0.000) with primer KMS12. The highest (44.2) Mean Average Percentage Difference (MAPD) was observed with primer KMS11 and lowest (9.98) with primer KMS6. The phylogenetic tree based on Nei's formula revealed that the primers used for the study were able to show the genetic similarity and diversity within and between breeds.


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