TITLE

Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana: methanol versus water

AUTHOR(S)
Pól, Jaroslav; Ostrá, Elena Varadˇová; Karásek, Pavel; Roth, Michal; Benešová, Karolínka; Kotlaříková, Pavla; Čáslavský, Josef
PUB. DATE
August 2007
SOURCE
Analytical & Bioanalytical Chemistry;Aug2007, Vol. 388 Issue 8, p1847
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Pressurised fluid extraction using water or methanol was employed for the extraction of stevioside from Stevia rebaudiana Bertoni. The extraction method was optimised in terms of temperature and duration of the static or the dynamic step. Extracts were analysed by liquid chromatography followed by UV and mass-spectrometric (MS) detections. Thermal degradation of stevioside was the same in both solvents within the range 70–160 °C. Methanol showed better extraction ability for isolation of stevioside from Stevia rebaudiana leaves than water within the range 110–160 °C. However, water represents the green alternative to methanol. The limit of detection of stevioside in the extract analysed was 30 ng for UV detection and 2 ng for MS detection.
ACCESSION #
25905071

 

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