Acid phosphatase production by recombinant Arxula adeninivorans

Minocha, V.; Kaur, Parvinder; Satyanarayana, T.; Kunze, G.
August 2007
Applied Microbiology & Biotechnology;Aug2007, Vol. 76 Issue 2, p387
Academic Journal
Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett–Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these were optimized using response surface methodology of central composite design. The highest enzyme yields were attained in the medium with 3.9% sucrose and 1.6% peptone at pH 3.8. Because of optimization, 3.86- and 4.19-fold enhancement in enzyme production was achieved in shake flasks (17,054 U g−1 DYB) and laboratory fermenter (18,465 U g−1 DYB), respectively.


Related Articles

  • APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity. Parvinder Kaur; Anja Lingner; Bijinder Singh; Erik Böer; Jelena Polajeva; Gerhard Steinborn; Rüdiger Bode; Gerd Gellissen; Tulasi Satyanarayana; Gotthard Kunze // Antonie van Leeuwenhoek;Jan2007, Vol. 91 Issue 1, p45 

    Abstract??The extracellular acid phosphatase-encodingArxula adeninivorans APHO1gene was isolated using degenerated specific oligonucleotide primers in a PCR screening approach. The gene harbours an ORF of 1449?bp encoding a protein of 483?amino acids with a calculated molecular mass of 52.4?kDa....

  • MicroRNA-574-5p Was Pivotal for TLR9 Signaling Enhanced Tumor Progression via Down-Regulating Checkpoint Suppressor 1 in Human Lung Cancer. Qinchuan Li; Xiaoman Li; Zhongliang Guo; Feng Xu; Jingyan Xia; Zhongmin Liu; Tao Ren // PLoS ONE;Nov2012, Vol. 7 Issue 11, Special section p1 

    Accumulating data suggested that functional expression of Toll-like receptors (TLRs) in tumor cells was involved in tumor progression. Our previous study demonstrated that TLR9 signaling could enhance the tumor progression of human lung cancer cells in vitro and in vivo. We further showed that...

  • Acid phosphatase and invertase activities of Aspergillus niger. Pawar, Vaishali; Thaker, Vrinda // Mycoscience (Springer Science & Business Media B.V.);Sep2009, Vol. 50 Issue 5, p323 

    Aspergillus niger is widely used as an enzyme source in industries. Considering its enzymic potential, A. niger was studied for its acid phosphatase (EC, orthophosphoric monoester phosphohydrolase), and invertase (EC, β-fructofuranoside fructohydrolase) activity in defined...

  • Low-molecular-weight acid phosphatase (ACPI), obesity, and blood lipid levels in subjects with... Lucarini, N.; Antonacci, E. // Human Biology;Aug97, Vol. 69 Issue 4, p509 

    Presents information of low-molecular-weight acid phoshatase (ACPI) and its relation to the human body genetically and otherwise. Location of ACPI within the body; Consistence of samples of persons; Performed statistical analysis on ACPI; Results of analysis.

  • Do Cecidomyiidae galls of Aspidosperma spruceanum (Apocynaceae) fit the pre-established cytological and histochemical patterns? Oliveira, Denis Coelho; Magalhães, Thiago; Carneiro, Renê; Alvim, Marina; Isaias, Rosy // Protoplasma;Jun2010, Vol. 242 Issue 1-4, p81 

    Cecidomyiidae galls commonly present a zonation of tissues with lignified cell layers externally limiting a reserve tissue and internally limiting a specialized nutritive tissue next to the larval chamber. The cytological aspects of this specialized tissue indicate high metabolic activity as...

  • Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese. Karasu-Yalcin, S.; Senses-Ergul, S.; Ozbas, Z. Yesim // Mljekarstvo / Dairy;Jan-Mar2012, Vol. 62 Issue 1, p53 

    In this study, 146 yeast isolates were obtained from 45 Erzincan tulum cheese samples. By using API ID 32C test system and some complementary morphological, physiological and biochemical tests, 121 of the isolates could be identified at species level, while 12 of them were identified at genus...

  • Influence of Aluminum on Mineral Nutrition of the Ectomycorrhizal Fungi Pisolithus sp. and Cantharellus cibarius. Reddy, M. Sudhakara; Babita, K.; Gay, G.; Ramamurthy, V. // Water, Air & Soil Pollution;Mar2002, Vol. 135 Issue 1-4, p55 

    The influence of aluminum on the growth and mineral nutrition of two ectomycorrhizal fungi, Cantharellus cibarius and Pisolithus sp. was studied in vitro. The mycelial biomass of both fungi decreased as the concentration of Al increased in the culture medium but C. cibarius was more resistant...

  • Identification and characterization of regulatory elements of the human prostatic acid phosphate promoter. Zelivianski, Stanislav; Igawa, Tsukasa; Lim, Stephen; Taylor, Rodney; Ming-Fong Lin // Oncogene;5/23/2002, Vol. 21 Issue 23, p3696 

    Presents a study that cloned and characterized the promoter region of prostatic acid phosphatase (PAcP) to gain insight into the regulation of PAcP. Description of PAcP; Genes whose expression is restricted to prostate cells; Comparison of the functional activity of PAcP promoter in human...

  • Ask Dr. Mendelsohn. Mendelsohn, Robert S. // RN;Mar84, Vol. 47 Issue 3, p73 

    Answers health-related questions. Information on acid phosphatase testing; Recommendations for any patient who has been through an unsuccessful medical procedure.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics