TITLE

Easily fabricated magnetic traps for single-cell applications

AUTHOR(S)
Koschwanez, John H.; Carlson, Robert H.; Meldrum, Deirdre R.
PUB. DATE
April 2007
SOURCE
Review of Scientific Instruments;Apr2007, Vol. 78 Issue 4, p043105
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.
ACCESSION #
25684698

 

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