Precision genome surgery

Pingoud, Alfred; Silva, George H.
July 2007
Nature Biotechnology;Jul2007, Vol. 25 Issue 7, p743
Academic Journal
The article presents a study which examines the potential of zinc finger nucleases (ZFNs) for gene therapy. It is noted that ZFNs allows allow one to cleave DNA at a chosen target sequence. They also cleave prolifically at off-target sites, resulting in unacceptable toxicity. Moreover, the goal of gene therapy is to repair gene defects without otherwise modifying the genome.


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