TITLE

Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform

AUTHOR(S)
Owino, Joseph H.; Ignaszak, Anna; Al-Ahmed, Amir; Baker, Priscilla G. L.; Alemu, Hailemichael; Ngila, Jane Catherine; Iwuoha, Emmanuel I.
PUB. DATE
July 2007
SOURCE
Analytical & Bioanalytical Chemistry;Jul2007, Vol. 388 Issue 5/6, p1069
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was developed with AFB1antibody (AFB1-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of the Pt/PANi–PSSA electrode, the Pt/PANi–PSSA/AFB1-Ab immunosensor and Pt/PANi–PSSA/AFB1-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066 kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for the Pt/PANi–PSSA electrode to 1,066 kΩ for the Pt/PANi–PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg.
ACCESSION #
25603384

 

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