TITLE

Determination of molar absorptivity coefficients for major type-B trichothecenes and certification of calibrators for deoxynivalenol and nivalenol

AUTHOR(S)
Krska, Rudolf; Schubert-Ullrich, Patricia; Josephs, Ralf D.; Emteborg, Håkan; Buttinger, Gerhard; Pettersson, Hans; Van Egmond, Hans P.; Schothorst, Ronald C.; MacDonald, Susan; Chan, Danny
PUB. DATE
July 2007
SOURCE
Analytical & Bioanalytical Chemistry;Jul2007, Vol. 388 Issue 5/6, p1215
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This paper presents results from the European Commission-funded project Doncalibrant, the objective of which was to produce calibrators with certified mass fractions of the Fusarium toxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-Ac-DON), 15-acetyldeoxynivalenol (15-Ac-DON), and nivalenol (NIV), in acetonitrile. The calibrators, available in ampoules, were sufficiently homogeneous, with between-bottle variations ( s bb) of less than 2%. Long-term stability studies performed at four different temperatures between −18 and 40 °C revealed no significant negative trends (at a confidence level of 95%). Molar absorptivity coefficients (in L mol−1 cm−1) were determined for all four toxins (DON: 6805 ± 126, NIV: 6955 ± 205, 3-Ac-DON: 6983 ± 141, 15-Ac-DON: 6935 ± 142) on the basis of a mini-interlaboratory exercise. The overall uncertainty of the calibrators’ target values for DON and NIV were evaluated on the basis of gravimetric preparation data and include uncertainty contributions from possible heterogeneity, storage, and transport. The Doncalibrant project resulted in the production of calibrators for DON (IRMM-315) and NIV (IRMM-316) in acetonitrile with certified mass fractions of 25.1 ± 1.2 μg g−1 and 24.0 ± 1.1 μg g−1, respectively. Both CRMs became commercially available from the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) at the beginning of 2007.
ACCESSION #
25603358

 

Related Articles

  • European measurement comparison of 137Cs, 40K and 90Sr in milk powder. Spasova, Y.; Wätjen, U.; Altzitzoglou, T. // Journal of Radioanalytical & Nuclear Chemistry;Jul2008, Vol. 277 Issue 1, p211 

    Recently, the Institute for Reference Materials and Measurements (IRMM) has assumed responsibility for organizing regular measurement comparisons among those laboratories which provide radioactivity monitoring data from their country to authorities of the European Commission (EC) under various...

  • Update on Isotope Reference Materials and Measurements for Nuclear Safeguards at IRMM. Richter, S.; Aregbe, Y.; Jakopic, R.; Kühn, H.; Truyens, J.; Kraiem, M.; Verbruggen, A.; Bürger, S. // Proceedings of the Institute of Nuclear Materials Management Ann;2010, p1 

    The European Commission's Institute for Reference Materials and Measurements is tasked for providing isotope reference materials and reference measurements not only to the EU member states but even to the entire international safeguards community. This does not only include various types of...

  • Neutron transmission and capture of 241Am. Lampoudis, C.; Kopecky, S.; Plompen, A.; Schillebeeckx, P.; Wynants, R.; Gunsing, F.; Sage, C.; Bouland, O.; Noguere, G. // EPJ Web of Conferences;2013, Issue 42, p01004-p.1 

    A set of neutron transmission and capture experiments based on the Time Of Flight (TOF) technique, were performed in order to determine the 241Am capture cross section in the energy range from 0.01 eV to 1 keV. The GELINA facility of the Institute for Reference Materials and Measurements (IRMM)...

  • Round robin for uranium isotopic measurements by mass spectrometry. Granier, G.; Pointurier, F.; Chartier, F. // Journal of Radioanalytical & Nuclear Chemistry;Mar2009, Vol. 279 Issue 3, p875 

    A round-robin for uranium isotopic analysis in two solutions with different concentrations prepared and certified by the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) was organized by CETAMA, a division of the Commissariat à l’Énergie Atomique (CEA:...

  • Investigation of chemical changes in uranium oxyfluoride particles using secondary ion mass spectrometry. Kips, Ruth S.; Kristo, Michael J. // Journal of Radioanalytical & Nuclear Chemistry;Dec2009, Vol. 282 Issue 3, p1031 

    Understanding how environmental conditions may affect sample composition is critical to the interpretation of laboratory analyses from environmental sampling. We prepared a set of UO2F2 particle samples from the hydrolysis of UF6 and stored these samples in environmental chambers at different...

  • Effects of Bread Making and Wheat Germ Addition on the Natural Deoxynivalenol Content in Bread. Giménez, Isabel; Blesa, Jesús; Herrera, Marta; Ariño, Agustín // Toxins;Jan2014, Vol. 6 Issue 1, p394 

    Deoxynivalenol (DON, vomitoxin) is a type-B trichothecene mycotoxin produced by several field fungi such as Fusarium graminearum and Fusarium culmorum and known to have various toxic effects. This study investigated the effect of the bread making process on the stability of DON in common bread...

  • Fusarium Tri4 encodes a multifunctional oxygenase required for trichothecene biosynthesis. McCormick, Susan P.; Alexander, Nancy J.; Proctor, Robert H. // Canadian Journal of Microbiology;Jul2006, Vol. 52 Issue 7, p636 

    Fusarium graminearum and Fusarium sporotrichioides produce the trichothecene mycotoxins 15-acetyldeoxynivalenol and T-2 toxin, respectively. In both species, disruption of the P450 monooxygenase-encoding gene, Tri4, blocks production of the mycotoxins and leads to the accumulation of the...

  • Natural Occurrence of Mycotoxins and Mycotoxigenic Fungi on Libyan Corn with Special Reference to Mycotoxin Control. Youssef, M. S. // Research Journal of Toxins;May2009, Vol. 1 Issue 1, p8 

    Eleven corn samples (9.2%) out of 120 tested proved to be toxic. Aflatoxins B1 and B2 were detected in (2 samples, 9.1-13.9 μg kg-1 corn), aflatoxins B1, B2, G1 and G2 (4 samples, 7.6-16.4), T-2 toxin (3 samples, 8.4-10.2), diacetoxyscirpenol (5 samples, 6.2-9.5) and zearalenone, (5 samples,...

  • Characterisation of the Fusarium graminearum-Wheat Floral Interaction. Brown, Neil A.; Bass, Chris; Baldwin, Thomas K.; Huaigu Chen; Massot, Fabien; Carion, Pierre W. C.; Urban, Martin; Van De Meene, Allison M. L.; Hammond-Kosack, Kim E. // Journal of Pathogens;2011, p1 

    Fusarium Ear Blight is a destructive fungal disease of cereals including wheat and can contaminate the crop with various trichothecene mycotoxins. This investigation has produced a new β-glucuronidase (GUS) reporter strain that facilitates the quick and easy assessment of plant infection. The...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics