Combination of analytical and microbiological techniques to study the antimicrobial activity of a new active food packaging containing cinnamon or oregano against E. coli and S. aureus

Becerril, R.; Gómez-Lus, R.; Goñi, P.; López, P.; Nerín, C.
July 2007
Analytical & Bioanalytical Chemistry;Jul2007, Vol. 388 Issue 5/6, p1003
Academic Journal
The aim of this work is the optimization and application of a group of analytical and microbiological techniques in the study of the activity of essential oils (EOs) incorporated in a new antimicrobial packaging material and the research in depth of the interaction between the microbial cells and the individual compounds present in the active material. For this purpose the antimicrobial activity of the active packaging containing cinnamon or oregano was evaluated against E. coli and S. aureus. The vapour phase activity and the direct contact between the antimicrobial agents themselves, or once incorporated in the packaging material, and the microbial cells have been studied. The direct contact was studied using a broth dilution method. The vapour phase was evaluated by using a new method which involves the use of a filter disk containing the EOs. Furthermore, the kill time assay was used to determine the exposure time for the maximum efficiency in packaging, and transmission electron microscopy was used to investigate the antimicrobial activity and the possible mechanism of action against E. coli and S. aureus. Finally, the compounds absorbed by cells were identified. The results showed that the techniques used provide relevant information about the antibacterial activity of cinnamon and oregano in direct contact as well as in the vapour phase. The antimicrobial packaging showed a fast efficiency which supports its likely application as a food packaging material. Bacteria treated with EOs exhibit a wide range of significant abnormalities; these include formation of blebs, coagulation of cytoplasmatic constituents, collapse of the cell structure and lack of cytoplasmatic material. Some of these observations are correlated to the ability of some of these substances to disrupt envelop structure, especially the inner membrane. After an extraction from dead cells, cinnamaldehyde was detected by GC-MS in E. coli exposed to the active packaging containing cinnamon.


Related Articles

  • Assessment of antimicrobial activity of whey protein films incorporated with biocide plant-based essential oils. Badr, K. R.; Ahmed, Zahra S.; El-Gamal, M. S. // Journal of Applied Sciences Research;Apr2013, Vol. 9 Issue 4, p2811 

    Antimicrobial substances incorporated into edible films can control microbial contamination of foods by reducing the growth rate and/or inactivating target microorganisms. The aim of this work is to evaluate the antimicrobial activity of plant-derived essential oils EOs embedded into whey...

  • Synthesis and Biological Activity of Substituted Tetrachlorophthalic Acid Monoamides. Dolzhenko, A.; Kolotova, N.; Kotegov, V.; Syropyatov, B.; Vasilyuk, M.; Rudakova, G.; Novikova, V.; Feshin, V. // Pharmaceutical Chemistry Journal;Aug2005, Vol. 39 Issue 8, p409 

    A series of tetrachlorophthalic acid derivatives were obtained by acylating primary amines with tetrachlorophthalic anhydride under mild conditions. Antibacterial activity of the synthesized compounds with respect to Escherichia coli and Staphylococcus aureus was studied in vitro by the serial...

  • Antimicrobial Efficacy and Phytochemical Screening of Mushrooms, Lenzites Betulinus, and Coriolopsis Gallica Extracts. Fakoya, Soji; Folarin Oloketuyi, Sandra // TAF Preventive Medicine Bulletin;Nov/Dec2012, Vol. 11 Issue 6, p695 

    AIM: The spread of multidrug-resistant strains of bacteria makes it necessary to discover new classes of antibacterial and compounds that inhibit these resistant mechanisms. Hence, this study was conducted to evaluate the antimicrobial activities of Lenzites betulinus and Coriolopsis gallica...

  • ANTIMICROBIAL ACTIVITY OF SOME SPICES AGAINST SELECTED MICROBES. Mishra, Neeraj; Behal, K. K. // International Journal of Pharmacy & Pharmaceutical Sciences;2010 Supplement, Vol. 2 Issue S3, p187 

    The aim of the study is to assess the antimicrobial activity and to determine the Minimum Inhibitory Concentration (MIC) of various spice extract on some bacterial and fungal strains. The antimicrobial activity of alcoholic and aqueous extracts of asafoetida, ginger, cinnamon and cardamom...

  • COMPARATIVE EFFECTS OF ESSENTIAL OILS ON GROWTH OF Escherichia coli. Kalaba, Vesna; Kalaba, Dragana // Carpathian Journal of Food Science & Technology;2014, Vol. 6 Issue 2, p5 

    This paper examines the antimicrobial activity of essential oils: anise, fennel, mint, orange, cinnamon, eucalyptus and rosemary on the growth of Escherichia coli. Different concentrations of essential oils were prepared by dissolving with 96% ethanol (1:1 and 2:1) and their antimicrobial effect...

  • Carotenoids and flavonoids can impair the effectiveness of some antimicrobial drugs against clinical isolates of Escherichia coli and Staphylococcus aureus. Dos Santos, R.; Pimenta-Freire, G.; Dias-Souza, M. V. // International Food Research Journal;2015, Vol. 22 Issue 5, p1777 

    Patients are taking more control over their health, and attracted by contributions to human nutrition, the use of phytonutrients raised. Carotenoids and flavonoids are among the most studied and consumed chemical entities in this sense. However, patients are often unaware of the potential risks...

  • Antimicrobial Effects on Starch-Based Films Incorporated with Lysozymes. Khairuddin, Nozieana; Muhamad, Ida Idayu // Pertanika Journal of Science & Technology;Jan2009, Vol. 17 Issue 1, p1 

    An antimicrobial (AM) Active Packaging can be made by incorporating and immobilizing suitable AM agents into food package matrices and applying a bio switch concept. A starch-based film was prepared and incorporated with an antimicrobial agent, i.e. lysozyme with EDTA as a chelating agent. This...

  • Antimicrobial Activity and Chemical Composition of Albanian Oregano. NEZA, EDLIRA; CENTINI, MARISANNA; MAMOCI, ERJON // Albanian Journal of Agricultural Sciences;2015, Vol. 14 Issue 4, p326 

    Antimicrobial activity of methanolic and aqueous methanolic extracts of oregano was tested against: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Pseudomonas spp, Candida albicans ATCC 10231, Listeria monocytogenes ATCC 19111 and Salmonella typhimurium ATCC 14028. Antimicrobial...

  • Comparison of antimicrobial activity of herbs & spices and their phytochemical determination. Singh, Manoj Kumar; Singh, Namita // International Journal of Green Pharmacy (Medknow Publications & ;Jul-Sep2011, Vol. 5 Issue 3, p229 

    The antimicrobial activities of crude methanolic extracts of five spices and herbs viz. Cuminum Cyminum (Family: Apiaceae), Nigella sativa (Family: Ranunculaceae), Pimpinella anisum (Family: Apiaceae), Trachyspermum copticum (Family: Umbelliferae), and rhizome of Zingiber officinale (Family:...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics