Estudio in vitro de algunos factores relacionados con la formación de película adquirida

Francia, Catalina Melchora; Lissera, Rosa Guadalupe; Battellino, Luis José
January 2004
Medicina Oral (16656024);2004, Vol. 6 Issue 1, p5
Academic Journal
An experimental study was carried out incubating to 37 °C for 90 min. a mixture of hydroxyapatite and supernatant (12.000 g 15 min.) of mixed saliva (20 mg/mL) to establish the possible influence of the availability of proteins, the pH, certain ions, the temperature and the ionic force on the formation of acquired pellicle under conditions in vitro. The retention of saliva proteins by the hydroxyapatite occurs proportionally to the concentration of macromolecules, until the linearity tends to disappear when the offering of proteins is very high and the adsorbente remains saturated. The graphics that represent the quantity of proteins retained by unit of mass of hydroxyapatite in function of the contained one protein show that the formation of acquired pellicle obeys basically to a phenomenon of adsorption relatively specific. The quantity of proteins adsorbed by the hydroxyapatite reached its maximum to the 30 min. of incubation, to pH 6.0 and to the temperature of 24 °C. The incorporation of calcium ionic to the system of trial increased the formation of acquired pellicle in a proportional way to the concentration of the ion. The phosphate ion produced an inverse effect. Both phenomena correspond adequate with the mechanism proposed for the formation of the acquired pellicle. It is concluded that the production of this integument represents a complex phenomenon where participate diverse physical, physicochemical and chemical factors so much of the quality of the fluids oral as of the characteristics of the adsorbent and of the conditions in which develops the phenomenon.


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