Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar, Nina; Kopitar, Andreja N.; Ihan, Alojz; Klun, Irma Virant; Bokal, Eda Vrtačnik
February 2007
Journal of Assisted Reproduction & Genetics;Feb2007, Vol. 24 Issue 2/3, p91
Academic Journal
Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate. Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry. Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031). Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased percentage of ROS producing granulosa cells results in fewer oocytes retrieved and diminishes implantation rate.


Related Articles

  • Up-regulation of apoptosis by gonadotrophin-releasing hormone agonist in cultures of endometrial cells from women with symptomatic myomas. Jia-Li Kang; Dong-Yu Wang; Xiao-Xia Wang; Jia Yu // Human Reproduction;Sep2010, Vol. 25 Issue 9, p2270 

    BACKGROUND: The aim of the present study was to evaluate the effect of gonadotrophin-releasing hormone agonist (GnRH-a), which is widely used in the medical treatment of symptomatic myomas, on the rate of endometrial cell apoptosis in cultures from women with symptomatic myomas. METHODS: The...

  • Effects on ERK1/2, JNK and GADD45α.  // Fertility Weekly;8/27/2012, p9 

    The article focuses on a multi-center study that investigates the mechanism of gonadotrophin-releasing hormone (GnRH) antagonist-induced apoptosis on decidual stromal cells. It notes that its effects on the activation of extracellular signal regulated protein kinase (ERK1/2), c-Jun-N-terminal...

  • Use of hCG vs. GnRH Agonist for Triggering Maturation of Donor Oocytes.  // Fertility Weekly;11/23/2009, p5 

    The article presents a study which compares the performance of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) agonists in triggering the maturation of donor oocytes. The study was conducted on 44 egg donors whose their fertilization, implantation and pregnancy rates...

  • GnRH antagonists and endometrial receptivity in oocyte recipients: a prospective randomized trial. Prapas, Nikos; Tavaniotou, Asimina; Panagiotidis, Yannis; Prapa, Stella; Kasapi, Evangelia; Goudakou, Maria; Papatheodorou, Achilleas; Prapas, Yannis // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Feb2009, Vol. 18 Issue 2, p276 

    The effect that gonadotrophin-releasing hormone (GnRH) antagonists exert on endometrial receptivity has not yet been elucidated. GnRH antagonists might directly affect oocytes, the embryo and/or the endometrium. The aim of this study was to investigate the direct effect of GnRH antagonists on...

  • Premature progesterone elevation impairs implantation and live birth rates in GnRH-agonist IVF/ICSI cycles. Kiliçdag, Esra Bulgan; Haydardedeoglu, Bulent; Cok, Tayfun; Hacivelioglu, Servet Ozden; Bagis, Tayfun // Archives of Gynecology & Obstetrics;Jul2010, Vol. 281 Issue 4, p747 

    Our aim was to investigate the clinical significance of premature elevated progesterone levels in women with good ovarian response treated with long gonadotropin-releasing hormone agonist (GnRH-a) cycles and IVF. Premature elevated progesterone levels refer to a rise in serum progesterone levels...

  • Parthenogenic Blastocysts Derived from Cumulus-Free In Vitro Matured Human Oocytes. McElroy, Sohyun L.; Byrne, James A.; Chavez, Shawn L.; Behr, Barry; Hsueh, Aaron J.; Westphal, Lynn M.; Reijo Pera, Renee A. // PLoS ONE;2010, Vol. 5 Issue 6, p1 

    Background: Approximately 20% of oocytes are classified as immature and discarded following intracytoplasmic sperm injection (ICSI) procedures. These oocytes are obtained from gonadotropin-stimulated patients, and are routinely removed from the cumulus cells which normally would mature the...

  • Proestrous Surge of Gonadotropin-Releasing Hormone Secretion Inhibits Apoptosis of Anterior Pituitary Cells in Cycling Female Rats. Yin, Ping; Arita, Jun // Neuroendocrinology;2002, Vol. 76 Issue 5, p272 

    Estrogen affects apoptotic cell death in estrogen-responsive tissues. The purpose of the present study was to examine dynamic changes in apoptotic cell death in the anterior pituitary gland during the estrous cycle and to investigate neuroendocrine regulation of these changes in cycling female...

  • The Role of GnRH Analogues in Endometriosis-Associated Apoptosis and Angiogenesis. Tesone, Marta; Bilotas, Mariela; Barañao, Rosa Inés; Meresman, Gabriela // Gynecologic & Obstetric Investigation;Oct2008 Supplement, Vol. 66, p10 

    It has been postulated that gonadotropin-releasing hormone (GnRH) analogues may act directly on endometrial cells and inhibit their growth and proliferation by regulation of apoptotic and angiogenic mechanisms. Eutopic endometrial cells from patients with endometriosis show an increased...

  • Blastocyst-stage versus cleavage-stage embryo transfer in women with high oestradiol concentrations: randomized controlled trial. Elgindy, Eman A.; Abou-Setta, Ahmed M.; Mostafa, Magdy I. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Dec2011, Vol. 23 Issue 6, p789 

    This prospective, randomized, controlled trial tested the hypothesis that delaying embryo transfer to the blastocyst stage can increase the probability of clinical pregnancy and live birth in women with high oestradiol concentrations on the day of human chorionic gonadotrophin (HCG) undergoing...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics