Derivatization of organophosphorus nerve agent degradation products for gas chromatography with ICPMS and TOF-MS detection

Richardson, Douglas D.; Caruso, Joseph A.
June 2007
Analytical & Bioanalytical Chemistry;Jun2007, Vol. 388 Issue 4, p809
Academic Journal
Separation and detection of seven V-type (venomous) and G-type (German) organophosphorus nerve agent degradation products by gas chromatography with inductively coupled plasma mass spectrometry (GC–ICPMS) is described. The nonvolatile alkyl phosphonic acid degradation products of interest included ethyl methylphosphonic acid (EMPA, VX acid), isopropyl methylphosphonic acid (IMPA, GB acid), ethyl hydrogen dimethylamidophosphate sodium salt (EDPA, GA acid), isobutyl hydrogen methylphosphonate (IBMPA, RVX acid), as well as pinacolyl methylphosphonic acid (PMPA), methylphosphonic acid (MPA), and cyclohexyl methylphosphonic acid (CMPA, GF acid). N-( tert-Butyldimethylsilyl)- N-methyltrifluroacetamide with 1% TBDMSCl was utilized to form the volatile TBDMS derivatives of the nerve agent degradation products for separation by GC. Exact mass confirmation of the formation of six of the TBDMS derivatives was obtained by GC–time of flight mass spectrometry (TOF-MS). The method developed here allowed for the separation and detection of all seven TBDMS derivatives as well as phosphate in less than ten minutes. Detection limits for the developed method were less than 5 pg with retention times and peak area precisions of less than 0.01 and 6%, respectively. This method was successfully applied to river water and soil matrices. To date this is the first work describing the analysis of chemical warfare agent (CWA) degradation products by GC–ICPMS. [Figure not available: see fulltext.]


Related Articles

  • Improvements of the Fluoride Reactivation Method for the Verification of Nerve Agent Exposure. Degenhardt, Carla E.A.M.; Pleijsier, Kees; van der Schans, Marcel J.; Langenberg, Jan P.; Preston, Kerry E.; Solano, Maria I.; Maggio, V.L.; Barr, John R. // Journal of Analytical Toxicology;Jul/Aug2004, Vol. 28 Issue 5, p364 

    Describes improvements of the fluoride-induced reactivation in human plasma or serum samples by enhancing the sample preparation with new solid-phase extraction cartridges and the mass spectrometry (MS) analysis with large volume injections. Biomarkers for the verification of organophosphorous...

  • Quantitation of Metabolites of the Nerve Agents Sarin, Soman, Cyclohexylsarin, VX, and Russian VX in Human Urine Using Isotope-Dilution Gas Chromatography—Tandem Mass Spectrometry. Barr, John R.; Driskell, W. Jack; Aston, Linda S.; Martinez, Rodolfo A. // Journal of Analytical Toxicology;Jul/Aug2004, Vol. 28 Issue 5, p372 

    Discusses the development of an isotope-dilution gas chromatography-tandem mass spectrometric method for quantitating the urinary metabolites of the organophosphorus nerve agents sarin (Gb), soman (GD), VX, Russian VX (RVX) and cyclohexylsarin (GF). Limits of detection; Threat of...

  • On-line GPC-GC Analysis of Organophosphorus Pesticides in Edible Oils. Jongenotter, Bert; Jansseen, Hans-Gerd // LC-GC Europe;Jun2002, Vol. 15 Issue 6, p338 

    Investigates the efficiency of an on-line gel permeation chromatography-gas chromatography method. Effectivity in determining the quantity of organophosphorus pesticides in crude edible oils; Quantification on the level of organophosphorus components by capillary gas chromatography; Use of a...

  • Determination of nerve agent metabolites in human urine by isotope-dilution gas chromatography-tandem mass spectrometry after solid phase supported derivatization. Lin, Ying; Chen, Jia; Yan, Long; Guo, Lei; Wu, Bidong; Li, Chunzheng; Feng, Jianlin; Liu, Qin; Xie, Jianwei // Analytical & Bioanalytical Chemistry;Aug2014, Vol. 406 Issue 21, p5213 

    A simple and sensitive method has been developed and validated for determining ethyl methylphosphonic acid (EMPA), isopropyl methylphosphonic acid (IMPA), isobutyl methylphosphonic acid (iBuMPA), and pinacolyl methylphosphonic acid (PMPA) in human urine using gas chromatography-tandem mass...

  • Photochemical synthesis of prebiotic phosphonic acids. Graaf, R.; Visscher, J. // Origins of Life & Evolution of the Biosphere;1996, Vol. 26 Issue 3-5, p351 

    An abstract of the paper "Photochemical Synthesis of Prebiotic Phosphonic Acids," by R. M. de Graaf and J. Visscher presented at the triennial meeting of the International Society for the Study of the Origin of Life (ISSOL) in July 1996 in Orléans, France is provided.

  • CHEMICAL WEAPONS and Their EFFECTS. Gay, Kathlyn // Silent Death;2001, p15 

    This chapter discusses the effects of chemical weapons. There are various types of chemical agents used for weapons. Nerve agents block the transmission of nerve impulses and paralyze the nervous system. When used as weapons, chemical agents are usually dispensed from aircraft or from cruise...

  • Protests grow over nerve gas disposal. Anderson, Ian // New Scientist;8/11/90, Vol. 127 Issue 1729, p18 

    Reports that environmental groups are trying to obtain an injunction in Hawaii to stop the shipment of chemical weapons to Johnston Atoll, where they are to be burned. Opposition by Pacific island nations; Lack of property authorization by the U.S. Army from the Environmental Protection Agency...

  • Structural Factors Influencing Potency of Currently Used Acetylcholinesterase Reactivators for Treatment of Cyclosarin Intoxications. Kuca, Kamil; Jun, Daniel; Bajgar, Jiri // Current Pharmaceutical Design;Nov2007, Vol. 13 Issue 33, p2445 

    Cyclosarin is one member of nerve agent family. Recent treatment of intoxications by organophosphorus compounds, such as nerve agents or pesticides, consists of rapid administration of anticholinergics and AChE reactivators. Owing to the threat of terroristic use of these compounds during last...

  • Efficacy of the rePON1 mutant IIG1 to prevent cyclosarin toxicity in vivo and to detoxify structurally different nerve agents in vitro. Worek, Franz; Seeger, Thomas; Goldsmith, Moshe; Ashani, Yacov; Leader, Haim; Sussman, Joel; Tawfik, Dan; Thiermann, Horst; Wille, Timo // Archives of Toxicology;Jun2014, Vol. 88 Issue 6, p1257 

    The potent human toxicity of organophosphorus (OP) nerve agents calls for the development of effective antidotes. Standard treatment for nerve agent poisoning with atropine and an oxime has a limited efficacy. An alternative approach is the development of catalytic bioscavengers using...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics