TITLE

Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad

AUTHOR(S)
Angel B., Rafael-Oswaldo; Chinchilla C., Misael; Guerrero B., Olga Marta; Castro C., Alfredo
PUB. DATE
March 2007
SOURCE
Revista de Biología Tropical;mar2007, Vol. 55 Issue 1, p121
SOURCE TYPE
Periodical
DOC. TYPE
Article
ABSTRACT
Two Trypanosoma cruzi isolates, TCR-4 from Costa Rica and UES-1 from El Salvador, were studied in vitro to compare their infectivity or resistance and intracellular replication in myocardial cells in three strains of mice and rats: NGP white mice, C3 H mice and Sprague Dowley rats. Myocardial cells were cultured on coverslips at 37 ° C in a humid 10 % CO2 atmosphere and then infected at a ratio of one tripomastigote per cell. Samples were studied after 24, 72, 96 and 120 h of infection to determine parasite infection capacity and intracellular multiplication. Both parasites had the highest infection capacity in C3 H mice, followed by NGP mice cells with a very low infection rate. Lastly, almost no Trypanosoma cruzi multiplication was observed in Sprague Dowley rats, suggesting a strong natural resistance in this animal to both strains of the parasite. The UES-1 isolate presented higher multiplication and greater invasion than the TCR-4 strain, showing greater virulence of UES-1 in heart cells, at least in vitro.
ACCESSION #
25245157

 

Related Articles

  • In Vitro and In Vivo Investigation of the Efficacy of Arylimidamide DB1831 and Its Mesylated Salt Form - DB1965 - against Trypanosoma cruzi Infection. da Silva, Cristiane Fran�a; da Gama Jaen Batista, Denise; Oliveira, Gabriel Melo; de Souza, Elen Mello; Hammer, Erica Ripoll; da Silva, Patricia Bernardino; Daliry, Anissa; Araujo, Julianna Siciliano; Britto, Constan�a; Rodrigues, Ana Carolina Mondaine; Zongying Liu; Farahat, Abdelbasset A.; Kumar, Arvind; Boykin, David W.; de Nazar� Correia Soeiro, Maria // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Chagas disease is caused by infection with the intracellular protozoan parasite Trypanosoma cruzi. At present, nifurtimox and benznidazole, both compounds developed empirically over four decades ago, represent the chemotherapeutic arsenal for treating this highly neglected disease. However, both...

  • In Vitro and In Vivo Investigation of the Efficacy of Arylimidamide DB1831 and Its Mesylated Salt Form - DB1965 - against Trypanosoma cruzi Infection. da Silva, Cristiane França; da Gama Jaen Batista, Denise; Oliveira, Gabriel Melo; de Souza, Elen Mello; Hammer, Erica Ripoll; da Silva, Patricia Bernardino; Daliry, Anissa; Araujo, Julianna Siciliano; Britto, Constança; Rodrigues, Ana Carolina Mondaine; Zongying Liu; Farahat, Abdelbasset A.; Kumar, Arvind; Boykin, David W.; de Nazaré Correia Soeiro, Maria // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Chagas disease is caused by infection with the intracellular protozoan parasite Trypanosoma cruzi. At present, nifurtimox and benznidazole, both compounds developed empirically over four decades ago, represent the chemotherapeutic arsenal for treating this highly neglected disease. However, both...

  • Expression of calpain-like proteins and effects of calpain inhibitors on the growth rate of Angomonas deanei wild type and aposymbiotic strains. de Oliveira, Simone Santiago Carvalho; Garcia-Gomes, Aline dos Santos; d'Avila-Levy, Claudia Masini; dos Santos, André Luis Souza; Branquinha, Marta Helena // BMC Microbiology;9/29/2015, Vol. 15 Issue 1, p1 

    Background: Angomonas deanei is a trypanosomatid parasite of insects that has a bacterial endosymbiont, which supplies amino acids and other nutrients to its host. Bacterium loss induced by antibiotic treatment of the protozoan leads to an aposymbiotic strain with increased need for amino acids...

  • Toll-like receptor-2 and interleukin-6 mediate cardiomyocyte protection from apoptosis during Trypanosoma cruzi murine infection. Ponce, Nicolás; Cano, Roxana; Carrera-Silva, Eugenio; Lima, Ana; Gea, Susana; Aoki, Maria // Medical Microbiology & Immunology;May2012, Vol. 201 Issue 2, p145 

    Local innate immunity plays a key role in initiating and coordinating homeostatic and defense responses in the heart. We have previously reported that the cardiotropic parasite Trypanosoma cruzi, the etiological agent of Chagas disease, protects cardiomyocytes against growth factor...

  • Chagas disease control: deltamethrin-treated collars reduce Triatoma infestans feeding success on dogs Reithinger, Richard; Ceballos, Leonardo; Stariolo, Raúl; Davies, Clive R.; Gürtler, Ricardo E. // Transactions of the Royal Society of Tropical Medicine & Hygiene;Jul2005, Vol. 99 Issue 7, p502 

    Summary: Dogs are domestic reservoir hosts of Trypanosoma cruzi, the aetiological agent of Chagas disease. Using an experimental set-up mimicking rural mud-and-thatch houses, we evaluated the effect of deltamethrin-treated dog collars on the feeding success and survival of Triatoma infestans,...

  • Correction: Activity In Vivo of Anti-Trypanosoma cruzi Compounds Selected from a High Throughput Screening.  // PLoS Neglected Tropical Diseases;Oct2014, Vol. 8 Issue 10, p1 

    A correction to the article "Activity In Vivo of Anti-Trypanosoma cruzi Compounds Selected from a High Throughput Screening" by Grasiella Andriani, Anne-Danielle C. Chessler, Gilles Courtemanche and colleagues published in the previous issue is presented.

  • His-tagged tryparedoxin peroxidase of Trypanosoma cruzi as a tool for drug screening. Guerrero, S. A.; Lopez, J. A.; Steinert, P.; Montemartini, M.; Kalisz, H. M.; Colli, W.; Singh, M.; Alves, M. J. M.; Flohé, L. // Applied Microbiology & Biotechnology;Apr2000, Vol. 53 Issue 4, p410 

    Tryparedoxin peroxidase has recently been identified as a constituent of the complex peroxidase system in the trypanosomatid Crithidia fasciculata [Nogoceke E, Gommel DU, Kiess M, Kalisz HM, Flohé L (1997) Biol Chem 378: 827–836]. In trypanosomatids, hydroperoxides are reduced at the...

  • PREVALENCE AND MOLECULAR PHYLOGENETIC CHARACTERIZATION OF TRYPANOSOMA (MEGATRYPANUM) MINASENSE IN THE PERIPHERAL BLOOD OF SMALL NEOTROPICAL PRIMATES AFTER A QUARANTINE PERIOD. Sato, Hiroshi; Leo, Natalie; Katakai, Yuko; Takano, Jun-ichiro; Akari, Hirofumi; Nakamura, Shin-ichiro; Une, Yumi // Journal of Parasitology;Oct2008, Vol. 94 Issue 5, p1128 

    Neotropical primates of the Cebidae and Callitrichidae, in their natural habitats, are frequently infected with a variety of trypanosomes including Trypanosoma cruzi, which causes a serious zoonosis, Chagas' disease. The state of trypanosome infection after a 30-day quarantine period was...

  • Trypanocidal Activity of Long Chain Diamines and Aminoalcohols. Legarda-Ceballos, Ana L.; del Olmo, Esther; López-Abán, Julio; Escarcena, Ricardo; Bustos, Luis A.; Fonseca-Berzal, Cristina; Gómez-Barrio, Alicia; Dib, Juan C.; San Feliciano, Arturo; Muro, Antonio // Molecules;Jun2015, Vol. 20 Issue 6, p11554 

    Thirteen aminoalcohols and eight diamines were obtained and tested against Trypanosoma cruzi epimastigotes strains MG, JEM and CL-B5 clone. Some of them were equal or more potent (1.0-6.6 times) than the reference compound nifurtimox. From them, three aminoalcohols and two diamines were selected...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics