TITLE

The Leukemoid Reaction in Clostridium sordellii Infection: Neuraminidase Induction of Promyelocytic Cell Proliferation

AUTHOR(S)
Aldape, Michael John; Bryant, Amy Evelyn; Yongsheng Ma; Stevens, Dennis Leroy
PUB. DATE
June 2007
SOURCE
Journal of Infectious Diseases;6/15/2007, Vol. 195 Issue 12, p1838
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Life-threatening Clostridium sordellii infections have recently been reported in women undergoing therapeutic abortion, during natural childbirth, and in injection drug users. Shock, diffuse capillary leak, and a leukemoid reaction (LR) are cardinal features of these infections. The magnitude of the LR is highly correlated with mortality. We have isolated a 42-kDa extractable protein from C. sordellii culture supernatant that stimulates proliferation of promyelocytic HL-60 cells in vitro. Using mass spectrometry, we have identified this protein as the C. sordellii neuraminidase, NanS. Recombinant NanS (rNanS) dose dependently stimulated HL-60 cell proliferation. Increased proliferation was observed when HL-60 cells were cocultured with both rNanS and granulocyte-macrophage colony stimulating factor. In addition, NanS also modified vascular cell adhesion molecule 1, which orchestrates the release of mature and immature granulocytes from bone marrow stromal cells. Thus, neuraminidase likely plays an important role in the characteristic LR in C. sordellii infection.
ACCESSION #
25095422

 

Related Articles

  • Ex vivo zidovudine (AZT) treatment of CD34+ bone marrow progenitors causes decreased steady state mitochondrial DNA (mtDNA) and increased lactate production. Lewis, L.D.; Amin, S.; Civin, C.I.; Lietman, P.S. // Human & Experimental Toxicology;Apr2004, Vol. 23 Issue 4, p173 

    Haematopoietic suppression is one of the dose-limiting side effects of chronic zidovudine (AZT) therapy. We tested the hypothesis that AZT would reduce mitochondrial DNA (mtDNA) content in haematopoietic progenitors causing impaired haematopoiesis and mitochondrial dysfunction. We studied the...

  • Lonafarnib reduces the resistance of primitive quiescent CML cells to imatinib mesylate in vitro. Jørgensen, H. G.; Allan, E. K.; Graham, S. M.; Godden, J. L.; Richmond, L.; Elliott, M. A.; Mountford, J. C.; Eaves, C. J.; Holyoake, T. L.; Jørgensen, H G // Leukemia (08876924);Jul2005, Vol. 19 Issue 7, p1184 

    Recent studies indicate that a rare population of primitive quiescent BCR-ABL(+) cells are innately insensitive to imatinib mesylate (IM) and persist after IM therapy of patients with chronic myeloid leukemia (CML). New approaches to the eradication of these cells are therefore likely to be...

  • THE EFFECT OF HYDROBIONT HALOCYNTHIA AURANTIUM EXTRACT ON HEMOPOIESIS UNDER CONDITIONS OF CYTOSTATIC MYELOSUPRESSION. Ponomareva, T. I.; Dobryakov, Yu. I.; Dobryakov, E. Yu. // Pacific Medical Journal / Tihookeanskij Medicinskij Zurnal;Mar2012, Issue 1, p50 

    The effect of haurantinum (extract of marine hydrobiont Halocynthia aurantium) on hemopoietic processes under cyclophosphanum (250 mg/kg) induced bone marrow hypoplasia in CBA mice were investigated. There was shown, that hemopoiesis recovery activated by haurantinum is manifested by the...

  • Proteomic analysis of the impact of static culturing on the expansion of rat bone marrow mesenchymal stem cells. Peter, Stefan; Evans, Caroline; Ow, Saw; Scutt, Andy; Wright, Phillip; Biggs, Catherine // Biotechnology Letters;Aug2012, Vol. 34 Issue 8, p1589 

    The clinical potential of mesenchymal stem cells (MSC) in tissue engineering and regenerative medicine is due to their self-renewal, proliferation and multi-lineage differentiation potential. Clinical use requires large cell numbers; which can, theoretically, be generated by ex vivo expansion of...

  • P13K-DEPENDENT ERK IS INVOLVED IN GM-CSF-MEDIATED ACTIVATION OF PROGENITOR CELLS IN A NEOVASCULARISATION MODEL. Wei Zhao; Xiang-Hong Yang // South African Journal of Science;May/Jun2010, Vol. 106 Issue 5/6, p1 

    The current study investigated the effects of the granulocyte-macrophage colony-stimulating factor (GM-CSF) on rat endothelial progenitor cell (EPC) biology. EPCs were isolated from rat bone marrow and experiments were carried out to determine the dose- and time-dependent effects of GM-CSF on...

  • The Effects of Hematopoietic Growth Factors on Neurite Outgrowth. Su, Ye; Cui, Lili; Piao, Chunshu; Li, Bin; Zhao, Li-Ru // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are initially discovered as the essential hematopoietic growth factors regulating bone marrow stem cell proliferation and differentiation, and SCF in combination with G-CSF (SCF+G-CSF) has synergistic effects on bone marrow...

  • Possible Use of Autologous Stem Cell Therapies for Alzheimer's Disease. Sugaya, Kiminobu // Current Alzheimer Research;Jul2005, Vol. 2 Issue 3, p367 

    The statement, �neurodegenerative diseases are incurable because neurons do not regenerate during adulthood,� has been challenged, and we have now found much evidence that the matured brain is capable of regenerating neurons. In our previous study, human neural stem cells (HNSCs)...

  • Selective Enzyme Inhibitors as Antileukemic Agents. Coleman, Mary Sue // BioScience;Dec1983, Vol. 33 Issue 11, p707 

    A clinical problem in antileukemic chemotherapy is that normal, rapidly proliferating cells of the marrow are destroyed along with malignant cells. Because children with hereditary lack of the enzyme adenosine deaminase suffer destruction of their lymphocytes, inhibitors of this enzyme have been...

  • Effect of alkaloidal fraction of Elaeocarpus ganitrus Roxb. seeds on murine in vitro immune parameters. Hule, Amolkumar Kisanrao; Juvekar, Archana Ramesh // Journal of Pharmacy Research;Aug2009, Vol. 2 Issue 8, p1261 

    The alkaloidal fraction of Elaeocarpus ganitrus seeds (EGAF) was prepared and evaluated for in vitro effects on murine cells for release of immune mediators and cell proliferation. The EGAF was tested at concentrations ranging between 6.5-832μg/ml. The release of mediators assayed from...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics